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Literature DB >> 334728

Isolation and genetic analysis of deletion mutants of colicin E1 plasmids carrying a TnA insertion.

Abstract

Deletions of colicin E1 (colE1) plasmid deoxyribonucleic acid (DNA) carrying the TnA transposon have been isolated. All except two were generated by nuclease digestion of plasmid DNA from its EcoRI-sensitive site. A plasmid containing about 16% of the ColE1 DNA (6.5 X 10(5) daltons) was generated that also contained the part of the TnA transposon conferring ampicillin resistance. The extents of different deletions were determined by analysis of restriction endonuclease fragments generated by the restriction endonucleases HaeII, BamHI, and HincII.

Entities: Chemical

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Year: 1977 PMID： 334728 PMCID： PMC221859 DOI： 10.1128/jb.132.1.321-331.1977

Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490

18 in total

Isolation and genetic analysis of deletion mutants of colicin E1 plasmids carrying a TnA insertion.

1. Isolation, mapping, and examination of effects of TnA insertions in ColE1 plasmids.

2. Studies of colicin E1 plasmid functions by analysis of deletions and TnA insertions of the plasmid.

3. Origin of replication of colicin E1 plasmid DNA.

4. Replication of colicin E1 plasmid DNA in cell extracts. Origin and direction of replication.

5. Replication of colicin E1 plasmid DNA in cell extracts. II. Selective synthesis of early replicative intermediates.

6. Unidirectional replication of plasmid ColE1 DNA.

7. Nature of Col E 1 plasmid replication in Escherichia coli in the presence of the chloramphenicol.

8. Cleavage map of colicin E1 plasmid.

9. Deletions affecting the transposition of an antibiotic resistance gene.

10. Plasmid ColEl as a molecular vehicle for cloning and amplification of DNA.

1. The control region of the F sex factor DNA transfer cistrons: physical mapping by deletion analysis.

2. Studies of colicin E1 plasmid functions by analysis of deletions and TnA insertions of the plasmid.

3. Nucleotide sequence of the region required for maintenance of colicin E1 plasmid.

4. flu, a metastable gene controlling surface properties of Escherichia coli.

5. Interactions between the F conjugal transfer system and CloDF13::Tna plasmids.

6. ColE1 copy number mutants.

7. ColE1 plasmid incompatibility: localization and analysis of mutations affecting incompatibility.

8. Location of an ampicillin resistance transposon, Tn1701, in a group of small, nontransferring plasmids.

9. Isolation and characterization of replication-deficient mutants of ColE1 plasmids.

10. Proteins synthesized in minicells containing plasmid ColE1 and its mutants.