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Literature DB >> 375200

Construction and analysis of recombinant DNAs containing a structural gene for rat prolactin.

E J Gubbins, R A Maurer, J L Hartley, J E Donelson.

Abstract

Poly A-containing RNA enriched in prolactin-coding sequences was isolated from female rate pituitaries after induction with diethylstilbesterol. Double stranded cDNA was synthesized from this RNA and inserted into plasmid pBR322 at the Pst I site via the poly(dG):polyy(dC) tailing method. E. coli was transformed with this DNA and the recombinant plasmid in one of the transformants characterized in detail. About half of its 900 base pair cDNA insert was sequenced. The DNA sequence is consistent with most of the reported amino acid sequence of rat preprolactin. In addition, the recombinant plasmids in two of the other transformants appear to contain growth hormone coding sequences.

Entities: Chemical Disease Gene Species

Mesh：

Substances：

Year: 1979 PMID： 375200 PMCID： PMC327742 DOI： 10.1093/nar/6.3.915

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

24 in total

1. A new method for sequencing DNA.

Authors: A M Maxam; W Gilbert
Journal: Proc Natl Acad Sci U S A Date: 1977-02 Impact factor: 11.205

2. A bacterial clone synthesizing proinsulin.

Authors: L Villa-Komaroff; A Efstratiadis; S Broome; P Lomedico; R Tizard; S P Naber; W L Chick; W Gilbert
Journal: Proc Natl Acad Sci U S A Date: 1978-08 Impact factor: 11.205

3. pBR322 restriction map derived from the DNA sequence: accurate DNA size markers up to 4361 nucleotide pairs long.

Authors: J G Sutcliffe
Journal: Nucleic Acids Res Date: 1978-08 Impact factor: 16.971

4. Nucleotide sequence of the ampicillin resistance gene of Escherichia coli plasmid pBR322.

Authors: J G Sutcliffe
Journal: Proc Natl Acad Sci U S A Date: 1978-08 Impact factor: 11.205

5. Nucleotide sequence and amplification in bacteria of structural gene for rat growth hormone.

Authors: P H Seeburg; J Shine; J A Martial; J D Baxter; H M Goodman
Journal: Nature Date: 1977-12-08 Impact factor: 49.962

6. Nucleotide sequence of part of the gene for human chorionic somatomammotropin: purification of DNA complementary to predominant mRNA species.

Authors: P H Seeburg; J Shine; J A Martial; A Ullrich; J D Baxter; H M Goodman
Journal: Cell Date: 1977-09 Impact factor: 41.582

7. Partial amino acid sequence of penicillinase coded by Escherichia coli plasmid R6K.

Authors: R P Ambler; G K Scott
Journal: Proc Natl Acad Sci U S A Date: 1978-08 Impact factor: 11.205

8. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.

Authors: F Bolivar; R L Rodriguez; P J Greene; M C Betlach; H L Heyneker; H W Boyer; J H Crosa; S Falkow
Journal: Gene Date: 1977 Impact factor: 3.688

9. Regulation of prolactin and somatotropin mRNAs by thyroliberin.

Authors: G A Evans; D N David; M G Rosenfeld
Journal: Proc Natl Acad Sci U S A Date: 1978-03 Impact factor: 11.205

10. Sequence organization and expression of a yeast plasmid DNA.

Authors: E J Gubbins; C S Newlon; M D Kann; J E Donelson
Journal: Gene Date: 1977-05 Impact factor: 3.688

14 in total

1. The rat prolactin gene family locus: species-specific gene family expansion.

Authors: S M Khorshed Alam; Rupasri Ain; Toshihiro Konno; Jennifer K Ho-Chen; Michael J Soares
Journal: Mamm Genome Date: 2006-08-04 Impact factor: 2.957

2. Differential regulation by estrogens of growth and prolactin synthesis in pituitary cells suggests that only a small pool of estrogen receptors is required for growth.

Authors: T Y Chun; D Gregg; D K Sarkar; J Gorski
Journal: Proc Natl Acad Sci U S A Date: 1998-03-03 Impact factor: 11.205

Review 10. Historical perspectives of prolactin and growth hormone as mammogens, lactogens and galactagogues--agog for the future!

Authors: Josephine F Trott; Barbara K Vonderhaar; Russell C Hovey
Journal: J Mammary Gland Biol Neoplasia Date: 2008-01-19 Impact factor: 2.673

Construction and analysis of recombinant DNAs containing a structural gene for rat prolactin.

1. A new method for sequencing DNA.

2. A bacterial clone synthesizing proinsulin.

3. pBR322 restriction map derived from the DNA sequence: accurate DNA size markers up to 4361 nucleotide pairs long.

4. Nucleotide sequence of the ampicillin resistance gene of Escherichia coli plasmid pBR322.

5. Nucleotide sequence and amplification in bacteria of structural gene for rat growth hormone.

6. Nucleotide sequence of part of the gene for human chorionic somatomammotropin: purification of DNA complementary to predominant mRNA species.

7. Partial amino acid sequence of penicillinase coded by Escherichia coli plasmid R6K.

8. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.

9. Regulation of prolactin and somatotropin mRNAs by thyroliberin.

10. Sequence organization and expression of a yeast plasmid DNA.

1. The rat prolactin gene family locus: species-specific gene family expansion.

2. Differential regulation by estrogens of growth and prolactin synthesis in pituitary cells suggests that only a small pool of estrogen receptors is required for growth.

3. Glucocorticoids antagonize induction of prolactin-gene expression by calcitriol in rat pituitary tumour cells.

4. Codon frequencies in 119 individual genes confirm consistent choices of degenerate bases according to genome type.

5. A bacterial cell that synthesizes a protein containing the antigenic determinants of rat prolactin.

6. Nucleotide sequence of a growth-related mRNA encoding a member of the prolactin-growth hormone family.

7. analysis of genomic rearrangements associated with two variable antigen genes of Trypanosoma brucei.

8. Genomic organization of rat prolactin and growth hormone genes.

9. Construction and characterization of a cDNA clone containing a portion of the bovine prolactin sequence.

Review 10. Historical perspectives of prolactin and growth hormone as mammogens, lactogens and galactagogues--agog for the future!