Literature DB >> 3658693

Solid phase synthesis of oligoribonucleotides using the o-nitrobenzyl group for 2'-hydroxyl protection and H-phosphonate chemistry.

T Tanaka1, S Tamatsukuri, M Ikehara.   

Abstract

Oligoribonucleotides with chain length of 7, 11, 15, 17, 24 and 34 were synthesized on long chain alkylamine controlled pore glass beads (LCA-CPG) using o-nitrobenzyl protection of 2'-hydroxyls via a H-phosphonate approach either manually or by using an automatic synthesizer. The oligoribonucleotides were obtained in yields of 0.6 0.6-20%, based on initial nucleoside bound to the LCA-CPG support.

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Year:  1987        PMID： 3658693      PMCID： PMC306245          DOI： 10.1093/nar/15.18.7235

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  8 in total

1.  Improved rapid phosphotriester synthesis of oligodeoxyribonucleotides using oxygen-nucleophilic catalysts.

Authors:  V A Efimov; O G Chakhmakhcheva
Journal:  Nucleic Acids Res       Date:  1985-05-24       Impact factor: 16.971

2.  DNA sequence analysis: a general, simple and rapid method for sequencing large oligodeoxyribonucleotide fragments by mapping.

Authors:  E Jay; R Bambara; R Padmanabhan; R Wu
Journal:  Nucleic Acids Res       Date:  1974-03       Impact factor: 16.971

3.  Solid phase synthesis of oligoribonucleotides using o-nitrobenzyl protection of 2'-hydroxyl via a phosphite triester approach.

Authors:  T Tanaka; S Tamatsukuri; M Ikehara
Journal:  Nucleic Acids Res       Date:  1986-08-11       Impact factor: 16.971

4.  Synthesis of DNA via deoxynucleoside H-phosphonate intermediates.

Authors:  B C Froehler; P G Ng; M D Matteucci
Journal:  Nucleic Acids Res       Date:  1986-07-11       Impact factor: 16.971

5.  Polymer support oligonucleotide synthesis XVIII: use of beta-cyanoethyl-N,N-dialkylamino-/N-morpholino phosphoramidite of deoxynucleosides for the synthesis of DNA fragments simplifying deprotection and isolation of the final product.

Authors:  N D Sinha; J Biernat; J McManus; H Köster
Journal:  Nucleic Acids Res       Date:  1984-06-11       Impact factor: 16.971

6.  Solid phase synthesis of polynucleotides. VI. Further studies on polystyrene copolymers for the solid support.

Authors:  H Ito; Y Ike; S Ikuta; K Itakura
Journal:  Nucleic Acids Res       Date:  1982-03-11       Impact factor: 16.971

7.  Highly efficient oligodeoxyribonucleotide synthesis using fully base protected phosphodiester building blocks carrying 2-(1-methylimidazol-2-yl) phenyl protection of the phosphate.

Authors:  B S Sproat; P Rider; B Beijer
Journal:  Nucleic Acids Res       Date:  1986-02-25       Impact factor: 16.971

8.  Rapid synthesis of oligodeoxyribonucleotides VI. Efficient, mechanised synthesis of heptadecadeoxyribonucleotides by an improved solid phase phosphotriester route.

Authors:  M L Duckworth; M J Gait; P Goelet; G F Hong; M Singh; R C Titmas
Journal:  Nucleic Acids Res       Date:  1981-04-10       Impact factor: 16.971
  8 in total
  10 in total

1.  Molecular design of a eukaryotic messenger RNA and its chemical synthesis.

Authors:  R Iwase; M Maeda; T Fujiwara; M Sekine; T Hata; K Miura
Journal:  Nucleic Acids Res       Date:  1992-04-11       Impact factor: 16.971

2.  Studies on the structure and stabilizing factor of the CUUCGG hairpin RNA using chemically synthesized oligonucleotides.

Authors:  T Sakata; H Hiroaki; Y Oda; T Tanaka; M Ikehara; S Uesugi
Journal:  Nucleic Acids Res       Date:  1990-07-11       Impact factor: 16.971

3.  Solid phase synthesis of the 5'-half of the initiator t-RNA from B. subtilis.

Authors:  C Chaix; A M Duplaa; D Molko; R Téoule
Journal:  Nucleic Acids Res       Date:  1989-09-25       Impact factor: 16.971

4.  Solid-phase synthesis of oligoribonucleotides using 9-fluorenylmethoxycarbonyl (Fmoc) for 5'-hydroxyl protection.

Authors:  C Lehmann; Y Z Xu; C Christodoulou; Z K Tan; M J Gait
Journal:  Nucleic Acids Res       Date:  1989-04-11       Impact factor: 16.971

5.  Synthesis and applications of oligoribonucleotides with selected 2'-O-methylation using the 2'-O-[1-(2-fluorophenyl)-4-methoxypiperidin-4-yl] protecting group.

Authors:  B Beijer; I Sulston; B S Sproat; P Rider; A I Lamond; P Neuner
Journal:  Nucleic Acids Res       Date:  1990-09-11       Impact factor: 16.971

6.  Evaluation of 2'-hydroxyl protection in RNA-synthesis using the H-phosphonate approach.

Authors:  E Rozners; E Westman; R Strömberg
Journal:  Nucleic Acids Res       Date:  1994-01-11       Impact factor: 16.971

7.  Solid phase synthesis of oligoribonucleotides using the 1-[(2-chloro-4-methyl)phenyl]-4-methoxypiperidin-4-yl (Ctmp) group for the protection of the 2'-hydroxy functions and the H-phosphonate approach.

Authors:  O Sakatsume; M Ohtsuki; H Takaku; C B Reese
Journal:  Nucleic Acids Res       Date:  1989-05-25       Impact factor: 16.971

8.  5'-Levulinyl and 2'-tetrahydrofuranyl protection for the synthesis of oligoribonucleotides by the phosphoramidite approach.

Authors:  S Iwai; E Ohtsuka
Journal:  Nucleic Acids Res       Date:  1988-10-25       Impact factor: 16.971

9.  Studies on the t-butyldimethylsilyl group as 2'-O-protection in oligoribonucleotide synthesis via the H-phosphonate approach.

Authors:  J Stawinski; R Strömberg; M Thelin; E Westman
Journal:  Nucleic Acids Res       Date:  1988-10-11       Impact factor: 16.971

10.  Synthesis and incorporation of the phosphoramidite derivative of 2'-O-photocaged 3'-s-thioguanosine into oligoribonucleotides: substrate for probing the mechanism of RNA catalysis.

Authors:  Nan-Sheng Li; Nicole Tuttle; Jonathan P Staley; Joseph A Piccirilli
Journal:  J Org Chem       Date:  2014-03-31       Impact factor: 4.354
  10 in total

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