Literature DB >> 361698

Cloned DNA fragment specifying major outer membrane protein a in Escherichia coli K-12.

R C Gayda, A Markovitz.   

Abstract

Plasmid pMC44 is a recombinant plasmid that contains a 2-megadalton EcoRI fragment of Escherichia coli K-12 DNA joined to the cloning vehicle, pSC101. The polypeptides specified by plasmid pMC44 were identified and compared with those specified by pSC101 to determine those that are unique to pMC44. Three polypeptides specified by plasmid pMC44 were localized in the cell envelope fraction of minicells: a Sarkosyl-insoluble outer membrane polypeptide (designated M2), specified by the cloned 2-megadalton DNA fragment, and two Sarkosyl-soluble membrane polypeptides specified by the cloning plasmid pSC101. Bacteria containing plasmid pMC44 synthesized quantities of M2 approximately equal to the most abundant E. coli K-12 outer membrane protein. Evidence is presented that outer membrane polypeptide M2, specified by the recombinant plasmid pMC44, is the normal E. coli outer membrane protein designated protein a by Lugtenberg and 3b by Schnaitman.

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Year:  1978        PMID： 361698      PMCID： PMC218669          DOI： 10.1128/jb.136.1.369-380.1978

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  31 in total

1.  Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis.

Authors:  D W Cleveland; S G Fischer; M W Kirschner; U K Laemmli
Journal:  J Biol Chem       Date:  1977-02-10       Impact factor: 5.157

2.  Outer membrane proteins of Escherichia coli. VI. Protein alteration in bacteriophage-resistant mutants.

Authors:  P J Bassford; D L Diedrich; C L Schnaitman; P Reeves
Journal:  J Bacteriol       Date:  1977-08       Impact factor: 3.490

3.  Proteins specified by herpes simplex virus. VI. Viral proteins in the plasma membrane.

Authors:  J W Heine; P G Spear; B Roizman
Journal:  J Virol       Date:  1972-03       Impact factor: 5.103

4.  Altered bacteriophage lambda expression in cell division mutants capR(lon) of Escherichia coli K-12.

Authors:  R C Gayda; A Markovitz
Journal:  Mol Gen Genet       Date:  1978-02-07

5.  New mini-ColE1 as a molecular cloning vehicle.

Authors:  H Avni; P E Berg; A Markovitz
Journal:  J Bacteriol       Date:  1977-01       Impact factor: 3.490

6.  Role of a sugar-lipid intermediate in colanic acid synthesis by Escherichia coli.

Authors:  J G Johnson; D B Wilson
Journal:  J Bacteriol       Date:  1977-01       Impact factor: 3.490

7.  On the nature of tetracycline resistance controlled by the plasmid pSC101.

Authors:  R C Tait; H W Boyer
Journal:  Cell       Date:  1978-01       Impact factor: 41.582

8.  Influence of cultural conditions and mutations on the composition of the outer membrane proteins of Escherichia coli.

Authors:  B Lugtenberg; R Peters; H Bernheimer; W Berendsen
Journal:  Mol Gen Genet       Date:  1976-09-23

9.  Protein expression in E. coli minicells by recombinant plasmids.

Authors:  R B Meagher; R C Tait; M Betlach; H W Boyer
Journal:  Cell       Date:  1977-03       Impact factor: 41.582

10.  Escherichia coli K-12 tolF mutants: alterations in protein composition of the outer membrane.

Authors:  T J Chai; J Foulds
Journal:  J Bacteriol       Date:  1977-05       Impact factor: 3.490
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  20 in total

1.  Cloning of the structural gene (ompA) for an integral outer membrane protein of Escherichia coli K-12.

Authors:  U Henning; H D Royer; R M Teather; I Hindennach; C P Hollenberg
Journal:  Proc Natl Acad Sci U S A       Date:  1979-09       Impact factor: 11.205

2.  C-shaped cells caused by expression of an ftsA mutation in Escherichia coli.

Authors:  R C Gayda; M C Henk; D Leong
Journal:  J Bacteriol       Date:  1992-08       Impact factor: 3.490

3.  Nucleotide sequence and intracellular location of the product of the fosfomycin resistance gene from transposon Tn2921.

Authors:  J Navas; J León; M Arroyo; J M García Lobo
Journal:  Antimicrob Agents Chemother       Date:  1990-10       Impact factor: 5.191

4.  omp T: Escherichia coli K-12 structural gene for protein a (3b).

Authors:  K R Rupprecht; G Gordon; M Lundrigan; R C Gayda; A Markovitz; C Earhart
Journal:  J Bacteriol       Date:  1983-02       Impact factor: 3.490

5.  Plasmid-determined immunity of Escherichia coli K-12 to colicin Ia Is mediated by a plasmid-encoded membrane protein.

Authors:  C A Weaver; A H Redborg; J Konisky
Journal:  J Bacteriol       Date:  1981-12       Impact factor: 3.490

6.  Cloning of gene lon (capR) of Escherichia coli K-12 and identification of polypeptides specified by the cloned deoxyribonucleic acid fragment.

Authors:  B A Zehnbauer; A Markovitz
Journal:  J Bacteriol       Date:  1980-08       Impact factor: 3.490

7.  Molecular cloning of the major outer membrane protein of Chlamydia trachomatis.

Authors:  I Allan; T M Cunningham; M A Lovett
Journal:  Infect Immun       Date:  1984-09       Impact factor: 3.441

8.  Neuroactive drugs inhibit trypsin and outer membrane protein processing in Escherichia coli K-12.

Authors:  R C Gayda; G W Henderson; A Markovitz
Journal:  Proc Natl Acad Sci U S A       Date:  1979-05       Impact factor: 11.205

9.  Identification of OmpT as the protease that hydrolyzes the antimicrobial peptide protamine before it enters growing cells of Escherichia coli.

Authors:  S Stumpe; R Schmid; D L Stephens; G Georgiou; E P Bakker
Journal:  J Bacteriol       Date:  1998-08       Impact factor: 3.490

10.  Mutations in genes cpxA and cpxB alter the protein composition of Escherichia coli inner and outer membranes.

Authors:  J McEwen; P M Silverman
Journal:  J Bacteriol       Date:  1982-09       Impact factor: 3.490
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