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Literature DB >> 318644

New mini-ColE1 as a molecular cloning vehicle.

Abstract

A new mini-ColE1 plasmid, designated pAC105, was isolated. It has a molecular weight of 1.6 X 10(6) and carries information for its self-replication as well as information for conferring colicin E1 immunity upon its host. Furthermore, pAC105 undergoes replication in the presence of chloramphenicol even when a foreign deoxyribonucleic acid (pSC101) is inserted into its single EcoRI restriction site. Studies in minicell-producing strains demonstrate that pAC105 codes for only two or three polypeptides of low molecular weight. The advantages of using it as a molecular cloning vehicle are discussed.

Entities: Chemical

Mesh：

Substances：

Year: 1977 PMID： 318644 PMCID： PMC234934 DOI： 10.1128/jb.129.1.358-366.1977

Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490

29 in total

Review 1. Colicinogeny and related phenomena.

Authors: K G Hardy
Journal: Bacteriol Rev Date: 1975-12

2. Mapping of colicin E2 and colicin E3 plasmid deoxyribonucleic acid EcoR-1-sensitive sites.

Authors: J Inselburg; V Johns
Journal: J Bacteriol Date: 1975-01 Impact factor: 3.490

Review 3. Production, properties and utility of bacterial minicells.

Authors: A C Frazer; R Curtiss
Journal: Curr Top Microbiol Immunol Date: 1975 Impact factor: 4.291

4. Discontinuous replication of colicin E1 plasmid deoxyribonucleic acid.

Authors: J Inselburg; A Oka
Journal: J Bacteriol Date: 1975-08 Impact factor: 3.490

5. The Genetic Basis for Mucoidy and Radiation Sensitivity in capR (lon) Mutants of E. coli K-12.

Authors: J W Bush; A Markovitz
Journal: Genetics Date: 1973-06 Impact factor: 4.562

6. Proteins specified by herpes simplex virus. V. Purification and structural proteins of the herpesvirion.

Authors: P G Spear; B Roizman
Journal: J Virol Date: 1972-01 Impact factor: 5.103

7. Construction of a colicin E1-R factor composite plasmid in vitro: means for amplification of deoxyribonucleic acid.

Authors: T Tanaka; B Weisblum
Journal: J Bacteriol Date: 1975-01 Impact factor: 3.490

8. Studies of mouse mitochondrial DNA in Escherichia coli: structure and function of the eucaryotic-procaryotic chimeric plasmids.

Authors: A C Chang; R A Lansman; D A Clayton; S N Cohen
Journal: Cell Date: 1975-10 Impact factor: 41.582

2. Isolation of a small DNA fragment carrying the gene for a dihydrofolate reductase from a trimethoprim resistance factor.

Authors: J W Zolg; U J Hänggi; H G Zachau
Journal: Mol Gen Genet Date: 1978-08-04

3. Replication of colicin E1 plasmid DNA in vivo requires no plasmid-encoded proteins.

Authors: D J Donoghue; P A Sharp
Journal: J Bacteriol Date: 1978-03 Impact factor: 3.490

4. Cloning of gene lon (capR) of Escherichia coli K-12 and identification of polypeptides specified by the cloned deoxyribonucleic acid fragment.

Authors: B A Zehnbauer; A Markovitz
Journal: J Bacteriol Date: 1980-08 Impact factor: 3.490

5. Sequence of the regulatory region of omp T, the gene specifying major outer membrane protein a (3b) of Escherichia coli K-12: implications for regulation and processing.

Authors: G Gordon; R C Gayda; A Markovitz
Journal: Mol Gen Genet Date: 1984

New mini-ColE1 as a molecular cloning vehicle.

Review 1. Colicinogeny and related phenomena.

2. Mapping of colicin E2 and colicin E3 plasmid deoxyribonucleic acid EcoR-1-sensitive sites.

Review 3. Production, properties and utility of bacterial minicells.

4. Discontinuous replication of colicin E1 plasmid deoxyribonucleic acid.

5. The Genetic Basis for Mucoidy and Radiation Sensitivity in capR (lon) Mutants of E. coli K-12.

6. Proteins specified by herpes simplex virus. V. Purification and structural proteins of the herpesvirion.

7. Construction of a colicin E1-R factor composite plasmid in vitro: means for amplification of deoxyribonucleic acid.

8. Studies of mouse mitochondrial DNA in Escherichia coli: structure and function of the eucaryotic-procaryotic chimeric plasmids.

9. Cloning of Escherichia coli DNA that controls cell division and capsular polysaccharide synthesis.

10. The generation of a ColE1-Apr cloning vehicle which allows detection of inserted DNA.

1. Nicking-closing enzyme assembles nucleosome-like structures in vitro.

2. Isolation of a small DNA fragment carrying the gene for a dihydrofolate reductase from a trimethoprim resistance factor.

3. Replication of colicin E1 plasmid DNA in vivo requires no plasmid-encoded proteins.

4. Cloning of gene lon (capR) of Escherichia coli K-12 and identification of polypeptides specified by the cloned deoxyribonucleic acid fragment.

5. Sequence of the regulatory region of omp T, the gene specifying major outer membrane protein a (3b) of Escherichia coli K-12: implications for regulation and processing.

6. DNA-stimulated ATPase activity on the lon (CapR) protein.

7. Identification of the gene lon (capR) product as a 94-kilodalton polypeptide by cloning and deletion analysis.

8. Cloned DNA fragment specifying major outer membrane protein a in Escherichia coli K-12.

9. Outer membrane protein a and other polypeptides regulate capsular polysaccharide synthesis in E. coli K-12.

10. Localization of genes responsible for replication and immunity to colicin A on plasmid ColA-CA31.