Adjustment and quantification of UV-visible spectrophotometry analysis: an accurate and rapid method for estimating Cladosporium spp. spore concentration in a water suspension.

Cladosporium spp. are among the most important plant pathogens, plant endophytes, insect parasites and human pathogens in nature. The aim of this study was to increase the speed and accuracy of Cladosporium spp. spore counting using UV-visible spectrophotometry based on the regression model in a water suspension. Spores of C. ramotenellum AM55, C. limoniforme Br15, C. tenuissimum K15 and C. cladosporioides Ld13 fungi were diluted in sterile distilled water several times. Spore concentration/ml (SC) was counted with a hemocytometer. The spectrophotometer visible light absorption (ABS) was measured under 14 wavelengths from 300 to 950 nm for each dilution. The results showed that the morphological variation of the spores greatly affect the determination of the suitable wavelength. 650, 750, 500 and 400 nm wavelengths had the highest coefficient of determination (R2) values respectively for C. ramotenellum AM55, C. limoniforme Br15, C. tenuissimum K15 and C. cladosporioides Ld13 on the linear regression model. R2 values were 0.9874, 0.9647, 0.8856 and 0.9711 respectively, for the 650, 750, 500 and 400 nm wavelengths. The linear equation of SC = 107 × ABS-133,040 with the highest R2 value of 0.9532 had the best fit under a combinatorial regression model where SC and ABS of all Cladosporium spp. were presented. The proposed linear regression models can be used under in vivo and in vitro conditions for medicine or plant pathology studies which certainly increase the accuracy and speed of the future experiments compared to the hemocytometer method.