Literature DB >> 2007647

Spectrophotometric method of inoculum preparation for the in vitro susceptibility testing of filamentous fungi.

A Espinel-Ingroff1, T M Kerkering.   

Abstract

Homogeneous inoculum suspensions of 29 isolates of clinically important filamentous fungi were adjusted with a spectrophotometer (530 nm) to obtain standardized preparations containing 1 x 10(6) to 5 x 10(6) CFU/ml. Colony counts (CFU per milliliter) of 1 x 10(6) to 5 x 10(6) were achieved on three different days for isolates of Aspergillus spp., Pseudallescheria boydii, and Sporothrix schenckii (80% +/- 2% transmission), and colony counts of 7 x 10(5) to 2.9 x 10(6) (70% +/- 2% transmission) were achieved for Mucor spp. and Rhizopus spp.

Entities:  

Mesh：

Year:  1991        PMID： 2007647      PMCID： PMC269774          DOI： 10.1128/jcm.29.2.393-394.1991

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  7 in total

1.  New fluorescence assay for the quantitation of fungi.

Authors:  T Coleman; J V Madassery; G S Kobayashi; M H Nahm; J R Little
Journal:  J Clin Microbiol       Date:  1989-09       Impact factor: 5.948

2.  Comparison of relative susceptibilities of Candida species to three antifungal agents as determined by unstandardized methods.

Authors:  J N Galgiani; J Reiser; C Brass; A Espinel-Ingroff; M A Gordon; T M Kerkering
Journal:  Antimicrob Agents Chemother       Date:  1987-09       Impact factor: 5.191

3.  Multicenter evaluation of four methods of yeast inoculum preparation.

Authors:  M A Pfaller; L Burmeister; M S Bartlett; M G Rinaldi
Journal:  J Clin Microbiol       Date:  1988-08       Impact factor: 5.948

4.  Standardization of yeast inocula with an electronic impedance counter.

Authors:  C Eng; P Valenstein
Journal:  J Clin Microbiol       Date:  1989-10       Impact factor: 5.948

5.  Turbidimetric studies of growth inhibition of yeasts with three drugs: inquiry into inoculum-dependent susceptibility testing, time of onset of drug effect, and implications for current and newer methods.

Authors:  J N Galgiant; D A Stevens
Journal:  Antimicrob Agents Chemother       Date:  1978-02       Impact factor: 5.191

6.  Collaborative investigation of variables in susceptibility testing of yeasts.

Authors:  M A Pfaller; M G Rinaldi; J N Galgiani; M S Bartlett; B A Body; A Espinel-Ingroff; R A Fromtling; G S Hall; C E Hughes; F C Odds
Journal:  Antimicrob Agents Chemother       Date:  1990-09       Impact factor: 5.191

7.  Variables influencing susceptibility testing of Cryptococcus neoformans to 5-fluorocytosine.

Authors:  E R Block; A E Jennings; J E Bennett
Journal:  Antimicrob Agents Chemother       Date:  1973-10       Impact factor: 5.191
  7 in total
  33 in total

1.  Inoculum standardization for antifungal susceptibility testing of filamentous fungi pathogenic for humans.

Authors:  E Petrikkou; J L Rodríguez-Tudela; M Cuenca-Estrella; A Gómez; A Molleja; E Mellado
Journal:  J Clin Microbiol       Date:  2001-04       Impact factor: 5.948

2.  Extracts of Flourensia cernua (L): volatile constituents and antifungal, antialgal, and antitermite bioactivities.

Authors:  M Tellez; R Estell; E Fredrickson; J Powell; D Wedge; K Schrader; M Kobaisy
Journal:  J Chem Ecol       Date:  2001-11       Impact factor: 2.626

3.  Interlaboratory evaluation of hematocytometer method of inoculum preparation for testing antifungal susceptibilities of filamentous fungi.

Authors:  J L Rodriguez-Tudela; Erja Chryssanthou; Evangelia Petrikkou; Juan Mosquera; David W Denning; Manuel Cuenca-Estrella
Journal:  J Clin Microbiol       Date:  2003-11       Impact factor: 5.948

4.  Nationwide survey of in vitro activities of itraconazole and voriconazole against clinical Aspergillus fumigatus isolates cultured between 1945 and 1998.

Authors:  Paul E Verweij; Debbie T A Te Dorsthorst; Anthonius J M M Rijs; Hilly G De Vries-Hospers; Jacques F G M Meis
Journal:  J Clin Microbiol       Date:  2002-07       Impact factor: 5.948

5.  Evaluation of possible correlations between antifungal susceptibilities of filamentous fungi in vitro and antifungal treatment outcomes in animal infection models.

Authors:  F C Odds; F Van Gerven; A Espinel-Ingroff; M S Bartlett; M A Ghannoum; M V Lancaster; M A Pfaller; J H Rex; M G Rinaldi; T J Walsh
Journal:  Antimicrob Agents Chemother       Date:  1998-02       Impact factor: 5.191

6.  Comparison of in vitro antifungal susceptibilities of conidia and hyphae of filamentous fungi.

Authors:  J Guarro; C Llop; C Aguilar; I Pujol
Journal:  Antimicrob Agents Chemother       Date:  1997-12       Impact factor: 5.191

7.  Multicenter evaluation of proposed standardized procedure for antifungal susceptibility testing of filamentous fungi.

Authors:  A Espinel-Ingroff; M Bartlett; R Bowden; N X Chin; C Cooper; A Fothergill; M R McGinnis; P Menezes; S A Messer; P W Nelson; F C Odds; L Pasarell; J Peter; M A Pfaller; J H Rex; M G Rinaldi; G S Shankland; T J Walsh; I Weitzman
Journal:  J Clin Microbiol       Date:  1997-01       Impact factor: 5.948

8.  Analysis of the influence of Tween concentration, inoculum size, assay medium, and reading time on susceptibility testing of Aspergillus spp.

Authors:  Alicia Gomez-Lopez; Amel Aberkane; Eva Petrikkou; Emilia Mellado; Juan Luis Rodriguez-Tudela; Manuel Cuenca-Estrella
Journal:  J Clin Microbiol       Date:  2005-03       Impact factor: 5.948

9.  Effect of increasing inoculum sizes of pathogenic filamentous fungi on MICs of antifungal agents by broth microdilution method.

Authors:  A Gehrt; J Peter; P A Pizzo; T J Walsh
Journal:  J Clin Microbiol       Date:  1995-05       Impact factor: 5.948

10.  Susceptibility testing of Candida albicans and Aspergillus species by a simple microtiter menadione-augmented 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide assay.

Authors:  B Jahn; E Martin; A Stueben; S Bhakdi
Journal:  J Clin Microbiol       Date:  1995-03       Impact factor: 5.948
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