Literature DB >> 25979149

Adjustment of a rapid method for quantification of Fusarium spp. spore suspensions in plant pathology.

Pablo F Caligiore-Gei1, Jorge G Valdez2.   

Abstract

The use of a Neubauer chamber is a broadly employed method when cell suspensions need to be quantified. However, this technique may take a long time and needs trained personnel. Spectrophotometry has proved to be a rapid, simple and accurate method to estimate the concentration of spore suspensions of isolates of the genus Fusarium. In this work we present a linear formula to relate absorbance measurements at 530nm with the number of microconidia/ml in a suspension.
Copyright © 2014 Asociación Argentina de Microbiología. Publicado por Elsevier España, S.L.U. All rights reserved.

Keywords:  Cuantificación de esporas; Espectrofotometría; Laboratory methods; Métodos de laboratorio; Spectrophotometry; Spore counting

Mesh:

Substances:

Year:  2015        PMID: 25979149     DOI: 10.1016/j.ram.2015.03.002

Source DB:  PubMed          Journal:  Rev Argent Microbiol        ISSN: 0325-7541            Impact factor:   1.852


  3 in total

1.  Adjustment and quantification of UV-visible spectrophotometry analysis: an accurate and rapid method for estimating Cladosporium spp. spore concentration in a water suspension.

Authors:  N Akbari Oghaz; S Hatamzadeh; K Rahnama; M Khorrami Moghaddam; S Vaziee; Z Tazik
Journal:  World J Microbiol Biotechnol       Date:  2022-08-12       Impact factor: 4.253

2.  Evaluation of the Copan ESwab Transport System for Viability of Pathogenic Fungi by Use of a Modification of Clinical and Laboratory Standards Institute Document M40-A2.

Authors:  Bharat Gandhi; Richard Summerbell; Tony Mazzulli
Journal:  J Clin Microbiol       Date:  2018-01-24       Impact factor: 5.948

3.  Linking physiological parameters with visible/near-infrared leaf reflectance in the incubation period of vascular wilt disease.

Authors:  Juan Carlos Marín-Ortiz; Nathalia Gutierrez-Toro; Verónica Botero-Fernández; Lilliana María Hoyos-Carvajal
Journal:  Saudi J Biol Sci       Date:  2019-05-31       Impact factor: 4.219

  3 in total

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