Literature DB >> 35895266

In Situ Imaging of mRNA Splicing Variants by SpliceRCA.

Xiaojun Ren1,2, Jinghong Li3.   

Abstract

We present a method for multiplexed in situ imaging of individual RNA splicing variants. This method enables quantifying RNA splicing variants with single-molecule resolution, discriminating splicing isoforms with single-base precision as well as analyzing the subcellular localization of transcripts. With this technology, it is possible to study cell heterogeneity of gene expression, potentially helping to decipher rich diversity in posttranscriptional function and assist clinical monitoring.
© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Entities:  

Keywords:  Fluorescence imaging; In situ imaging; RNA splicing variants; Rolling circle amplification

Mesh：

Substances：

Year:  2022        PMID： 35895266     DOI： 10.1007/978-1-0716-2521-7_12

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  19 in total

Review 1.  Alternative pre-mRNA splicing and proteome expansion in metazoans.

Authors:  Tom Maniatis; Bosiljka Tasic
Journal:  Nature       Date:  2002-07-11       Impact factor: 49.962

Review 2.  Alternative splicing as a regulator of development and tissue identity.

Authors:  Francisco E Baralle; Jimena Giudice
Journal:  Nat Rev Mol Cell Biol       Date:  2017-05-10       Impact factor: 94.444

3.  Single-molecule imaging of transcriptionally coupled and uncoupled splicing.

Authors:  Diana Y Vargas; Khyati Shah; Mona Batish; Michael Levandoski; Sourav Sinha; Salvatore A E Marras; Paul Schedl; Sanjay Tyagi
Journal:  Cell       Date:  2011-11-23       Impact factor: 41.582

Review 4.  Expansion of the eukaryotic proteome by alternative splicing.

Authors:  Timothy W Nilsen; Brenton R Graveley
Journal:  Nature       Date:  2010-01-28       Impact factor: 49.962

5.  Conditional control of alternative splicing through light-triggered splice-switching oligonucleotides.

Authors:  James Hemphill; Qingyang Liu; Rajendra Uprety; Subhas Samanta; Michael Tsang; Rudolph L Juliano; Alexander Deiters
Journal:  J Am Chem Soc       Date:  2015-03-03       Impact factor: 15.419

Review 6.  Mechanisms of alternative splicing regulation: insights from molecular and genomics approaches.

Authors:  Mo Chen; James L Manley
Journal:  Nat Rev Mol Cell Biol       Date:  2009-09-23       Impact factor: 94.444

7.  Variability and robustness in T cell activation from regulated heterogeneity in protein levels.

Authors:  Ofer Feinerman; Joël Veiga; Jeffrey R Dorfman; Ronald N Germain; Grégoire Altan-Bonnet
Journal:  Science       Date:  2008-08-22       Impact factor: 47.728

8.  Analyzing alternative splicing data of splice junction arrays from Parkinson patients' leukocytes before and after deep brain stimulation as compared with control donors.

Authors:  Lilach Soreq; Nathan Salomonis; Zvi Israel; Hagai Bergman; Hermona Soreq
Journal:  Genom Data       Date:  2015-07-16

9.  Single-chromosome transcriptional profiling reveals chromosomal gene expression regulation.

Authors:  Marshall J Levesque; Arjun Raj
Journal:  Nat Methods       Date:  2013-02-17       Impact factor: 28.547

10.  Non-genetic origins of cell-to-cell variability in TRAIL-induced apoptosis.

Authors:  Sabrina L Spencer; Suzanne Gaudet; John G Albeck; John M Burke; Peter K Sorger
Journal:  Nature       Date:  2009-04-12       Impact factor: 49.962
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