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Literature DB >> 351561

Detection of 5-methylcytosine in DNA sequences.

Abstract

Col E1 DNA has methylated cytosine in the sequence 5'-CC*(A/T)GG-3' and methylated adenine in the sequence 5'-GA*TC-3' at the positions indicated by asterisks(*). When the Maxam-Gilbert DNA sequencing method is applied to this DNA, the methylated cytosine (5-methylcytosine) is found to be less reactive to hydrazine than are cytosine and thymine, so that a band corresponding to that base does not appear in the pyrimidine cleavage patterns. The existence of the methylated cytosine can be confirmed by analyzing the complementary strand or unmethylated DNA. In contrast, the methylated adenine (probably N6-methyladenine) cannot be distinguished from adenine with standard conditions for cleavage at adenine.

Entities: Chemical

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Year: 1978 PMID： 351561 PMCID： PMC342097 DOI： 10.1093/nar/5.5.1479

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

16 in total

1. STUDIES OF THE NUCLEOTIDE ARRANGEMENT IN DEOXYRIBONUCLEIC ACIDS. IX. SELECTIVE DEGRADATION OF PYRIMIDINE DEOXYRIBONUCLEOTIDES.

Authors: A TEMPERLI; H TUERLER; P RUEST; A DANON; E CHARGAFF
Journal: Biochim Biophys Acta Date: 1964-11-15

2. Studies on the biological role of DNA methylation. II. Role of phiX174 DNA methylation in the process of viral progeny DNA synthesis.

Authors: J Friedman; A Razin
Journal: Nucleic Acids Res Date: 1976-10 Impact factor: 16.971

3. Complementary specificity of restriction endonucleases of Diplococcus pneumoniae with respect to DNA methylation.

Authors: S Lacks; B Greenberg
Journal: J Mol Biol Date: 1977-07 Impact factor: 5.469

4. Two sequence-specific endonucleases from Moraxella bovis.

Authors: R E Gelinas; P A Myers; R J Roberts
Journal: J Mol Biol Date: 1977-07 Impact factor: 5.469

5. A new method for sequencing DNA.

Authors: A M Maxam; W Gilbert
Journal: Proc Natl Acad Sci U S A Date: 1977-02 Impact factor: 11.205

6. Origin of replication of colicin E1 plasmid DNA.

Authors: J I Tomizawa; H Ohmori; R E Bird
Journal: Proc Natl Acad Sci U S A Date: 1977-05 Impact factor: 11.205

7. Recognition sequence of a restriction enzyme.

Authors: C H Bigger; K Murray; N E Murray
Journal: Nat New Biol Date: 1973-07-04

8. Location of DNA methylation genes on the Escherichia coli K-12 genetic map.

Authors: M G Marinus
Journal: Mol Gen Genet Date: 1973-12-14

9. DNA substrate site for the EcoRII restriction endonuclease and modification methylase.

Authors: H W Boyer; L T Chow; A Dugaiczyk; J Hedgpeth; H M Goodman
Journal: Nat New Biol Date: 1973-07-11

10. In vivo methylation by Escherichia coli K-12 mec+ deoxyribonucleic acid-cytosine methylase protects against in vitro cleavage by the RII restriction endonuclease (R. Eco RII).

Authors: S Schlagman; S Hattman; M S May; L Berger
Journal: J Bacteriol Date: 1976-05 Impact factor: 3.490

55 in total

1. Cloning and nucleotide sequence of the genes coding for the Sau96I restriction and modification enzymes.

Authors: L Szilák; P Venetianer; A Kiss
Journal: Nucleic Acids Res Date: 1990-08-25 Impact factor: 16.971

2. Methylation of B-hordein genes in barley endosperm is inversely correlated with gene activity and affected by the regulatory gene Lys3.

Authors: M B Sørensen
Journal: Proc Natl Acad Sci U S A Date: 1992-05-01 Impact factor: 11.205

3. Identification of a subdomain within DNA-(cytosine-C5)-methyltransferases responsible for the recognition of the 5' part of their DNA target.

Authors: C Lange; C Wild; T A Trautner
Journal: EMBO J Date: 1996-03-15 Impact factor: 11.598

Detection of 5-methylcytosine in DNA sequences.

1. STUDIES OF THE NUCLEOTIDE ARRANGEMENT IN DEOXYRIBONUCLEIC ACIDS. IX. SELECTIVE DEGRADATION OF PYRIMIDINE DEOXYRIBONUCLEOTIDES.

2. Studies on the biological role of DNA methylation. II. Role of phiX174 DNA methylation in the process of viral progeny DNA synthesis.

3. Complementary specificity of restriction endonucleases of Diplococcus pneumoniae with respect to DNA methylation.

4. Two sequence-specific endonucleases from Moraxella bovis.

5. A new method for sequencing DNA.

6. Origin of replication of colicin E1 plasmid DNA.

7. Recognition sequence of a restriction enzyme.

8. Location of DNA methylation genes on the Escherichia coli K-12 genetic map.

9. DNA substrate site for the EcoRII restriction endonuclease and modification methylase.

10. In vivo methylation by Escherichia coli K-12 mec+ deoxyribonucleic acid-cytosine methylase protects against in vitro cleavage by the RII restriction endonuclease (R. Eco RII).

1. Cloning and nucleotide sequence of the genes coding for the Sau96I restriction and modification enzymes.

2. Methylation of B-hordein genes in barley endosperm is inversely correlated with gene activity and affected by the regulatory gene Lys3.

3. Identification of a subdomain within DNA-(cytosine-C5)-methyltransferases responsible for the recognition of the 5' part of their DNA target.

Review 4. Identifying 5-methylcytosine and related modifications in DNA genomes.

5. Determination of methylation specificity of DsaV methyltransferase by a simple biochemical method.

6. Complete nucleotide sequence of the polymerase 3 gene of human influenza virus A/WSN/33.

7. Tissue specificity and clustering of methylated cystosines in bovine satellite I DNA.

8. Reduced cleavage by sodium hydroxide of methyladenine in DNA sequencing.

9. Nucleotide sequence of the region of an origin of replication of the antibiotic resistance plasmid R6K.

10. Sequence of the gene for isoleucine tRNA1 and the surrounding region in a ribosomal RNA operon of Escherichia coli.