Literature DB >> 35635711

Measuring Nuclear Organization of Proteins with STORM Imaging and Cluster Analysis.

Ália Dos Santos1, Rosemarie E Gough1, Lin Wang2, Christopher P Toseland3.   

Abstract

Super-resolution microscopy enables the high-precision localization of proteins. Therefore, it is possible to investigate the spatial organization of proteins within the nucleus to understand how their organization relates to regulation and function. Here, we present methodology for single-molecule localization microscopy and cluster analysis where we cover sample preparation, image acquisition, and data analysis.
© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Entities:  

Keywords:  Cluster analysis; Nuclear organization; Nucleus; STORM; Single molecule

Mesh：

Year:  2022        PMID： 35635711     DOI： 10.1007/978-1-0716-2221-6_20

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  10 in total

1.  Nuclear organization of DNA replication in primary mammalian cells.

Authors:  B K Kennedy; D A Barbie; M Classon; N Dyson; E Harlow
Journal:  Genes Dev       Date:  2000-11-15       Impact factor: 11.361

2.  Coordinate-based colocalization analysis of single-molecule localization microscopy data.

Authors:  Sebastian Malkusch; Ulrike Endesfelder; Justine Mondry; Márton Gelléri; Peter J Verveer; Mike Heilemann
Journal:  Histochem Cell Biol       Date:  2011-11-16       Impact factor: 4.304

3.  Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM).

Authors:  Michael J Rust; Mark Bates; Xiaowei Zhuang
Journal:  Nat Methods       Date:  2006-08-09       Impact factor: 28.547

Review 4.  Transcription factories: genome organization and gene regulation.

Authors:  Argyris Papantonis; Peter R Cook
Journal:  Chem Rev       Date:  2013-04-18       Impact factor: 60.622

Review 5.  Fluorescent labeling and modification of proteins.

Authors:  Christopher P Toseland
Journal:  J Chem Biol       Date:  2013-04-13

Review 6.  Biogenesis and function of nuclear bodies.

Authors:  Yuntao S Mao; Bin Zhang; David L Spector
Journal:  Trends Genet       Date:  2011-06-15       Impact factor: 11.639

Review 7.  The emerging role of nuclear architecture in DNA repair and genome maintenance.

Authors:  Tom Misteli; Evi Soutoglou
Journal:  Nat Rev Mol Cell Biol       Date:  2009-03-11       Impact factor: 94.444

8.  Clus-DoC: a combined cluster detection and colocalization analysis for single-molecule localization microscopy data.

Authors:  Sophie V Pageon; Philip R Nicovich; Mahdie Mollazade; Thibault Tabarin; Katharina Gaus
Journal:  Mol Biol Cell       Date:  2016-08-31       Impact factor: 4.138

9.  NDP52 activates nuclear myosin VI to enhance RNA polymerase II transcription.

Authors:  Natalia Fili; Yukti Hari-Gupta; Ália Dos Santos; Alexander Cook; Simon Poland; Simon M Ameer-Beg; Maddy Parsons; Christopher P Toseland
Journal:  Nat Commun       Date:  2017-11-30       Impact factor: 14.919

10.  DNA damage alters nuclear mechanics through chromatin reorganization.

Authors:  Ália Dos Santos; Alexander W Cook; Rosemarie E Gough; Martin Schilling; Nora A Olszok; Ian Brown; Lin Wang; Jesse Aaron; Marisa L Martin-Fernandez; Florian Rehfeldt; Christopher P Toseland
Journal:  Nucleic Acids Res       Date:  2021-01-11       Impact factor: 16.971
  10 in total
  1 in total

1.  Binding partners regulate unfolding of myosin VI to activate the molecular motor.

Authors:  Ália Dos Santos; Natalia Fili; Yukti Hari-Gupta; Rosemarie E Gough; Lin Wang; Marisa Martin-Fernandez; Jesse Aaron; Eric Wait; Teng-Leong Chew; Christopher P Toseland
Journal:  Biochem J       Date:  2022-07-15       Impact factor: 3.766
  1 in total

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