Literature DB >> 35599828

Datasets for grain protein content, yield-related traits, and candidate genes in a durum wheat RIL population derived from a "hexaploid × tetraploid" interspecific cross.

Angelica Giancaspro1, Stefania Lucia Giove1, Ilaria Marcotuli1, Agata Gadaleta1.   

Abstract

Data described in this article refer to the evaluation of genetic variability for quantity (grain protein content, GPC) and composition (HMW-glutenin subunits and gliadins) of seed storage proteins, and two yield components (grain yield per spike, GYS, and thousand-kernel weight, TKW) in a durum wheat recombinant inbred line (RIL) population derived by an interspecific cross between the common wheat accession 02-5B-318 and the durum cv. Saragolla. This article provides datasets relative to GPC, GYS and TKW collected in the two parents and in 135 durum RIL progenies from plants grown in field trials conducted in Valenzano (Metropolitan City of Bari, BA, Italy) by a randomized complete block design with three replicates. Data on GPC were acquired from Near-Infrared Reflectance on whole-meal flour and are expressed as percentage of proteins on a dry weight basis. Data relative to composition of seed storage proteins refer to high molecular weight glutenin subunits (encoded by Glu-A1 and Glu-B1 loci) and gliadins (encoded by Gli-B1 locus) extracted from whole-grain samples and identified based on their electrophoretic relative mobility on SDS-PAGE. This paper also provides datasets for the detection of quantitative trait loci (QTLs) for GPC, GYS, TKW on a durum wheat genetic linkage map previously developed in the same durum population genotyped with the Illumina 90 K iSelect SNP array. The present article finally supports information for the identification of candidate genes related to wheat grain quantity, composition, and yield by providing data relative to all the SNP markers mapped in the QTL confidence intervals for each trait of interest (GPC, GYS, TKW). Data described in this paper support the published original research article titled "Genetic variation for protein content and yield-related traits in a durum population derived from an inter-specific cross between hexaploid and tetraploid wheat cultivars" (Giancaspro et al., 2019).
© 2022 The Author(s). Published by Elsevier Inc.

Entities:  

Keywords:  Candidate gene; Durum wheat; GPC; Grain yield per spike; QTL; Thousand kernel weight

Year:  2022        PMID： 35599828      PMCID： PMC9118095          DOI： 10.1016/j.dib.2022.108234

Source DB:  PubMed          Journal:  Data Brief        ISSN： 2352-3409


Specifications Table

Azienda Agricola “Martucci” Valenzano (Metropolitan City of Bari, BA) Italy Lat. 41.0438° N, long. 16.8842° E, elevation 85 m above sea level. University of Bari “Aldo Moro”, Department of Agricultural and Environmental Science (DiSAAT) Metropolitan City of Bari (BA) Italy Lat. 41.12688° N, long. 16.86596° E, elevation 5 m above sea level.

Value of the Data

Datasets reported in the present article are valuable as they include original data on new genetic variability for seed storage protein quantity and quality originated by crossing the common wheat line 02-5B-318 and the durum cv Saragolla. Moreover, this paper supplies detailed protocols and references to allow experimental research reproducibility. Data described in this article are addressed to the scientific community working on durum wheat breeding. Scientists who can benefit from the reported datasets are especially those breeders interested in exploiting the genetic variability within the RIL population derived from the cross “02-5B-318 × Saragolla”. Data on GPC and seed storage protein composition reported in the present article can be used for further identification of high-quality wheat lines to be employed - directly or as donor genotypes - in breeding programs for the development of novel durum varieties with increased commercial and nutritional relevance. Data relative to QTL mapping and candidate genes can be helpful for further dissecting the molecular basis of wheat quality and yield-related traits. Particularly, these data may support the development of new QTL-associated genetic markers exploitable in MAS breeding programs.

Data Description

Table 1 reports data relative to grain protein content (GPC) of two parents and 135 durum RILs derived by the interspecific cross “02-5B-318 × Saragolla”. Data refer to wheat lines grown in replicated field trials at Valenzano (Metropolitan City of Bari, BA, Italy) in years 2015 (GPC_Val15), 2016 (GPC_Val16), and 2017 (GPC_Val17). Average of environments (GPC_Mean) is also reported. For each line, reported GPC is the mean of three field replicates, each averaged between two measurements. GPC is reported as protein percentage on dry matter calculated by the following formula: GPC% d.m. = [Tot. GPC% / (100 − H%)] × 100.
Table 1

Grain protein content (GPC) in two parents and 135 durum wheat RILs derived by the “02-5B-318 × Saragolla” interspecific cross. Data refer to three environments (GPC_Val15; GPC_Val16; GPC_Val17) and the average of environments (GPC_Mean). Reported values represent the mean of three field replicates, each averaged between two measurements. % d.m. = percentage proteins on dry matter calculated as: [Tot. GPC% / (100 - H%)] × 100.

GenotypeLineLine nameGPC_Val15 (% d.m.)GPC_Val16 (% d.m.)GPC_Val17 (% d.m.)GPC_mean (% d.m.)
CommonParent02-5B-31813.8814.4512.3713.57
DurumParentSaragolla12.3317.1213.2514.23
DurumRIL (F7:F8)116.4515.7413.5615.25
DurumRIL (F7:F8)217.0917.5913.8216.17
DurumRIL (F7:F8)315.2513.8212.9914.02
DurumRIL (F7:F8)418.4116.2714.5716.42
DurumRIL (F7:F8)5–16.7912.4114.60
DurumRIL (F7:F8)616.2818.7913.3416.14
DurumRIL (F7:F8)716.3418.2113.5516.04
DurumRIL (F7:F8)817.5717.1413.4216.05
DurumRIL (F7:F8)9––––
DurumRIL (F7:F8)1015.3016.5012.8214.87
DurumRIL (F7:F8)1114.3913.4413.8913.91
DurumRIL (F7:F8)1215.0714.6514.4114.71
DurumRIL (F7:F8)1315.5717.8714.2215.89
DurumRIL (F7:F8)1417.9915.5915.2616.28
DurumRIL (F7:F8)1514.3714.9214.3314.54
DurumRIL (F7:F8)1618.6418.3716.0417.69
DurumRIL (F7:F8)1715.4515.7214.8015.32
DurumRIL (F7:F8)1818.6316.7115.2316.86
DurumRIL (F7:F8)1915.5317.1413.7215.46
DurumRIL (F7:F8)2018.2717.5315.5617.12
DurumRIL (F7:F8)2114.3816.4113.7314.84
DurumRIL (F7:F8)2213.3414.9713.2113.84
DurumRIL (F7:F8)2313.9916.3514.3214.89
DurumRIL (F7:F8)2415.3915.3413.2714.67
DurumRIL (F7:F8)2516.7417.5914.3216.22
DurumRIL (F7:F8)2614.7115.6014.5514.96
DurumRIL (F7:F8)2713.2416.2213.4314.30
DurumRIL (F7:F8)2813.8015.7113.7814.43
DurumRIL (F7:F8)29––––
DurumRIL (F7:F8)3016.4114.2214.9515.19
DurumRIL (F7:F8)3117.5015.5616.6716.58
DurumRIL (F7:F8)3214.4516.2814.8815.20
DurumRIL (F7:F8)3315.1515.7713.9414.95
DurumRIL (F7:F8)3417.7317.3415.0816.72
DurumRIL (F7:F8)3517.0716.4915.5816.38
DurumRIL (F7:F8)3617.2716.5116.3016.69
DurumRIL (F7:F8)3717.9917.6417.6517.76
DurumRIL (F7:F8)3816.5517.3415.9616.62
DurumRIL (F7:F8)3918.9816.2217.8817.69
DurumRIL (F7:F8)4019.5719.1215.7718.15
DurumRIL (F7:F8)4115.7815.5714.8815.41
DurumRIL (F7:F8)42––––
DurumRIL (F7:F8)4317.9314.4316.1816.18
DurumRIL (F7:F8)4417.0114.8215.2715.70
DurumRIL (F7:F8)4515.0015.3514.6515.00
DurumRIL (F7:F8)4616.4218.3314.7916.51
DurumRIL (F7:F8)4718.4119.7716.2118.13
DurumRIL (F7:F8)4817.4015.4213.7315.52
DurumRIL (F7:F8)4915.4715.1513.8814.83
DurumRIL (F7:F8)50––––
DurumRIL (F7:F8)5114.9715.4914.9515.13
DurumRIL (F7:F8)52––––
DurumRIL (F7:F8)5314.4716.5413.8014.94
DurumRIL (F7:F8)54––––
DurumRIL (F7:F8)5517.7416.8015.2316.59
DurumRIL (F7:F8)5619.3117.2115.9017.47
DurumRIL (F7:F8)57–18.2018.1218.16
DurumRIL (F7:F8)5815.4717.2515.2415.98
DurumRIL (F7:F8)5916.3214.7214.8715.30
DurumRIL (F7:F8)6014.1613.8414.6814.22
DurumRIL (F7:F8)6115.6515.7514.8515.42
DurumRIL (F7:F8)6218.4018.8015.7117.64
DurumRIL (F7:F8)63––––
DurumRIL (F7:F8)64––––
DurumRIL (F7:F8)6517.2215.4115.5316.05
DurumRIL (F7:F8)6613.9414.4814.6314.35
DurumRIL (F7:F8)6715.9218.6714.8816.49
DurumRIL (F7:F8)6818.6018.9215.3317.62
DurumRIL (F7:F8)6914.7416.7114.8215.42
DurumRIL (F7:F8)7016.7314.7615.1815.56
DurumRIL (F7:F8)7116.0416.4614.2915.60
DurumRIL (F7:F8)7217.1916.5116.3316.68
DurumRIL (F7:F8)7316.3619.0014.7316.70
DurumRIL (F7:F8)7414.7616.3814.2315.12
DurumRIL (F7:F8)7517.2919.0514.7317.03
DurumRIL (F7:F8)7615.0816.6314.5415.42
DurumRIL (F7:F8)7716.7216.0615.6216.13
DurumRIL (F7:F8)7814.4815.0414.7214.75
DurumRIL (F7:F8)7917.4517.0415.6116.70
DurumRIL (F7:F8)8018.3618.6816.2517.76
DurumRIL (F7:F8)8116.5616.5016.1416.40
DurumRIL (F7:F8)8217.1018.3415.3716.94
DurumRIL (F7:F8)8317.0415.9116.0216.32
DurumRIL (F7:F8)8418.3116.4415.6416.80
DurumRIL (F7:F8)85––14.0514.05
DurumRIL (F7:F8)8615.4015.8416.0315.76
DurumRIL (F7:F8)8716.3816.1215.0815.86
DurumRIL (F7:F8)8817.1816.9815.5516.57
DurumRIL (F7:F8)8918.8115.4513.7015.99
DurumRIL (F7:F8)90––––
DurumRIL (F7:F8)91–16.9813.3515.17
DurumRIL (F7:F8)9219.5316.7316.9317.73
DurumRIL (F7:F8)9317.4416.1017.7017.08
DurumRIL (F7:F8)9417.1015.6117.0716.59
DurumRIL (F7:F8)9516.9918.7114.9416.88
DurumRIL (F7:F8)9613.6013.6212.9613.39
DurumRIL (F7:F8)9722.1020.1815.9819.42
DurumRIL (F7:F8)9815.9517.0315.2616.08
DurumRIL (F7:F8)9914.6315.9615.4315.34
DurumRIL (F7:F8)10016.9016.6414.9516.16
DurumRIL (F7:F8)10115.2916.2814.6315.40
DurumRIL (F7:F8)10216.9416.1213.5715.54
DurumRIL (F7:F8)103––––
DurumRIL (F7:F8)10416.6015.7915.1615.85
DurumRIL (F7:F8)105––––
DurumRIL (F7:F8)10617.9417.7514.8716.85
DurumRIL (F7:F8)10715.7917.9414.2115.98
DurumRIL (F7:F8)108––––
DurumRIL (F7:F8)109–17.0116.6016.81
DurumRIL (F7:F8)11014.7514.0715.3714.73
DurumRIL (F7:F8)11117.9219.1215.9417.66
DurumRIL (F7:F8)11218.4414.9616.6016.67
DurumRIL (F7:F8)11318.8318.6716.5718.02
DurumRIL (F7:F8)11418.6317.4616.8317.64
DurumRIL (F7:F8)115––16.3416.34
DurumRIL (F7:F8)11616.8916.7615.2716.31
DurumRIL (F7:F8)11718.6521.1417.2219.00
DurumRIL (F7:F8)11814.9416.8014.5815.44
DurumRIL (F7:F8)11916.0516.9014.8815.95
DurumRIL (F7:F8)12014.3415.7215.4815.18
DurumRIL (F7:F8)12117.5516.2015.4916.41
DurumRIL (F7:F8)12217.2817.4715.6416.80
DurumRIL (F7:F8)12320.0520.2516.2418.84
DurumRIL (F7:F8)124–17.6916.8917.29
DurumRIL (F7:F8)12516.6516.6114.0715.78
DurumRIL (F7:F8)126–19.4815.2517.36
DurumRIL (F7:F8)12714.5615.2814.4114.75
DurumRIL (F7:F8)12818.4916.7716.9217.39
DurumRIL (F7:F8)12912.1814.6913.9213.60
DurumRIL (F7:F8)13014.7414.5014.5314.59
DurumRIL (F7:F8)13119.6619.2816.2118.38
DurumRIL (F7:F8)13216.2816.7715.3516.13
DurumRIL (F7:F8)13314.1014.5815.4214.70
DurumRIL (F7:F8)13414.7416.2214.9615.30
DurumRIL (F7:F8)135––––
Grain protein content (GPC) in two parents and 135 durum wheat RILs derived by the “02-5B-318 × Saragolla” interspecific cross. Data refer to three environments (GPC_Val15; GPC_Val16; GPC_Val17) and the average of environments (GPC_Mean). Reported values represent the mean of three field replicates, each averaged between two measurements. % d.m. = percentage proteins on dry matter calculated as: [Tot. GPC% / (100 - H%)] × 100. Table 2 lists data relative to grain yield per spike (GYS) of two parents and 135 durum RILs derived by the interspecific cross “02-5B-318 × Saragolla”. Data refer to wheat lines grown in replicated field trials at Valenzano (Metropolitan City of Bari, BA, Italy) in years 2015 (GYS_Val15), 2016 (GYS_Val16), and 2017 (GYS_Val17). Average of environments (GYS_Mean) is also reported. For each line, reported GYS is the mean of three field replicates, each averaged between two measurements.
Table 2

Grain yield per spike (GYS) in two parents and 135 durum wheat RILs derived by the “02-5B-318 × Saragolla” interspecific cross. Data refer to three environments (GYS_Val15; GYS_Val16; GYS_Val17) and the average of environments (GYS_Mean). Reported values represent the mean of three field replicates, each averaged between two measurements.

GenotypeLineLine nameGYS_Val15 (g)GYS_Val16 (g)GYS_Val17 (g)GYS_mean (g)
CommonParent02-5B-3181.301.470.991.25
DurumParentSaragolla2.262.781.682.24
DurumRIL (F7:F8)11.171.191.071.14
DurumRIL (F7:F8)21.070.741.211.01
DurumRIL (F7:F8)31.261.581.031.29
DurumRIL (F7:F8)40.991.240.811.01
DurumRIL (F7:F8)50.911.851.881.55
DurumRIL (F7:F8)61.181.631.001.27
DurumRIL (F7:F8)71.611.461.591.55
DurumRIL (F7:F8)81.110.931.251.10
DurumRIL (F7:F8)9––––
DurumRIL (F7:F8)101.151.141.221.17
DurumRIL (F7:F8)112.40–1.411.91
DurumRIL (F7:F8)121.441.571.341.45
DurumRIL (F7:F8)131.311.101.331.25
DurumRIL (F7:F8)140.731.060.630.81
DurumRIL (F7:F8)151.191.401.241.28
DurumRIL (F7:F8)160.460.710.640.60
DurumRIL (F7:F8)171.631.681.301.54
DurumRIL (F7:F8)181.050.930.990.99
DurumRIL (F7:F8)191.131.471.221.27
DurumRIL (F7:F8)200.791.211.191.06
DurumRIL (F7:F8)211.501.601.491.53
DurumRIL (F7:F8)221.512.181.591.76
DurumRIL (F7:F8)231.912.451.501.95
DurumRIL (F7:F8)241.671.861.531.69
DurumRIL (F7:F8)251.441.401.211.35
DurumRIL (F7:F8)261.711.920.991.54
DurumRIL (F7:F8)271.301.721.081.37
DurumRIL (F7:F8)281.481.821.451.58
DurumRIL (F7:F8)29––––
DurumRIL (F7:F8)301.301.220.711.08
DurumRIL (F7:F8)310.720.850.530.70
DurumRIL (F7:F8)321.641.451.361.48
DurumRIL (F7:F8)331.481.311.071.29
DurumRIL (F7:F8)341.401.561.111.36
DurumRIL (F7:F8)351.352.091.101.51
DurumRIL (F7:F8)360.931.150.911.00
DurumRIL (F7:F8)371.291.400.681.12
DurumRIL (F7:F8)381.60–1.101.35
DurumRIL (F7:F8)390.72–0.470.60
DurumRIL (F7:F8)400.43–0.790.61
DurumRIL (F7:F8)411.001.320.711.01
DurumRIL (F7:F8)42––––
DurumRIL (F7:F8)430.880.970.520.79
DurumRIL (F7:F8)440.640.930.650.74
DurumRIL (F7:F8)451.151.150.891.06
DurumRIL (F7:F8)461.300.981.221.17
DurumRIL (F7:F8)471.291.290.981.19
DurumRIL (F7:F8)481.462.221.541.74
DurumRIL (F7:F8)492.061.811.341.74
DurumRIL (F7:F8)50––––
DurumRIL (F7:F8)511.381.490.991.29
DurumRIL (F7:F8)52––––
DurumRIL (F7:F8)531.631.131.291.35
DurumRIL (F7:F8)54––––
DurumRIL (F7:F8)551.051.181.381.20
DurumRIL (F7:F8)560.700.990.870.85
DurumRIL (F7:F8)570.261.110.590.65
DurumRIL (F7:F8)581.231.201.151.19
DurumRIL (F7:F8)591.691.801.131.54
DurumRIL (F7:F8)600.851.220.890.99
DurumRIL (F7:F8)611.391.851.221.49
DurumRIL (F7:F8)620.300.490.500.43
DurumRIL (F7:F8)63––––
DurumRIL (F7:F8)640.871.160.890.97
DurumRIL (F7:F8)65-–––
DurumRIL (F7:F8)661.351.701.011.35
DurumRIL (F7:F8)671.310.751.101.05
DurumRIL (F7:F8)680.810.991.040.95
DurumRIL (F7:F8)691.290.991.201.16
DurumRIL (F7:F8)701.351.481.081.30
DurumRIL (F7:F8)711.441.461.181.36
DurumRIL (F7:F8)721.341.440.951.24
DurumRIL (F7:F8)731.311.171.371.28
DurumRIL (F7:F8)741.311.521.111.31
DurumRIL (F7:F8)750.910.651.330.96
DurumRIL (F7:F8)761.091.191.001.09
DurumRIL (F7:F8)771.322.050.901.42
DurumRIL (F7:F8)781.151.020.941.04
DurumRIL (F7:F8)791.191.550.951.23
DurumRIL (F7:F8)800.800.890.600.76
DurumRIL (F7:F8)811.421.520.941.29
DurumRIL (F7:F8)820.850.760.920.84
DurumRIL (F7:F8)830.841.010.710.85
DurumRIL (F7:F8)840.650.770.750.72
DurumRIL (F7:F8)85––1.831.83
DurumRIL (F7:F8)861.110.830.760.90
DurumRIL (F7:F8)871.201.190.841.08
DurumRIL (F7:F8)881.120.981.011.04
DurumRIL (F7:F8)890.750.901.030.89
DurumRIL (F7:F8)90-–––
DurumRIL (F7:F8)910.421.411.481.10
DurumRIL (F7:F8)920.521.250.790.85
DurumRIL (F7:F8)931.14–0.640.89
DurumRIL (F7:F8)940.791.520.781.03
DurumRIL (F7:F8)950.960.571.420.98
DurumRIL (F7:F8)961.391.491.211.36
DurumRIL (F7:F8)970.220.380.610.40
DurumRIL (F7:F8)981.071.451.481.33
DurumRIL (F7:F8)991.051.551.251.28
DurumRIL (F7:F8)1001.17–0.931.05
DurumRIL (F7:F8)1011.391.561.301.42
DurumRIL (F7:F8)1021.141.701.511.45
DurumRIL (F7:F8)1030.43––0.43
DurumRIL (F7:F8)1040.670.710.620.67
DurumRIL (F7:F8)105––––
DurumRIL (F7:F8)1060.620.941.030.86
DurumRIL (F7:F8)1071.390.781.261.14
DurumRIL (F7:F8)108––––
DurumRIL (F7:F8)1090.261.340.740.78
DurumRIL (F7:F8)1101.511.521.391.47
DurumRIL (F7:F8)1110.680.560.640.63
DurumRIL (F7:F8)1121.261.240.741.08
DurumRIL (F7:F8)1130.570.930.820.77
DurumRIL (F7:F8)1140.700.580.610.63
DurumRIL (F7:F8)1150.71–0.640.68
DurumRIL (F7:F8)1160.681.210.940.94
DurumRIL (F7:F8)1170.430.800.640.62
DurumRIL (F7:F8)1181.240.761.121.04
DurumRIL (F7:F8)1191.201.001.301.17
DurumRIL (F7:F8)1201.240.911.041.06
DurumRIL (F7:F8)1210.961.030.960.98
DurumRIL (F7:F8)1220.831.460.841.04
DurumRIL (F7:F8)1230.390.500.710.53
DurumRIL (F7:F8)1240.342.010.671.01
DurumRIL (F7:F8)1250.650.561.060.76
DurumRIL (F7:F8)126–0.871.000.94
DurumRIL (F7:F8)1271.341.581.151.36
DurumRIL (F7:F8)1280.931.420.641.00
DurumRIL (F7:F8)1291.471.181.131.26
DurumRIL (F7:F8)1301.341.440.921.23
DurumRIL (F7:F8)1310.970.920.900.93
DurumRIL (F7:F8)1320.75–0.890.82
DurumRIL (F7:F8)1331.321.340.871.18
DurumRIL (F7:F8)1341.281.171.101.18
DurumRIL (F7:F8)135––––
Grain yield per spike (GYS) in two parents and 135 durum wheat RILs derived by the “02-5B-318 × Saragolla” interspecific cross. Data refer to three environments (GYS_Val15; GYS_Val16; GYS_Val17) and the average of environments (GYS_Mean). Reported values represent the mean of three field replicates, each averaged between two measurements. Table 3 supplies data relative to thousand-kernel weight (TKW) of two parents and 135 durum RILs derived by the inter-specific cross “02-5B-318 × Saragolla”. Data refer to wheat lines grown in replicated field trials at Valenzano (Metropolitan City of Bari, BA, Italy) in years 2015 (TKW_Val15), 2016 (TKW_Val16), and 2017 (TKW_Val17). Average of environments (TKW_ Mean) is also reported. For each line, reported TKW is the mean of three field replicates, each averaged between two measurements.
Table 3

Thousand-kernel weight (TKW) in two parents and 135 durum wheat RILs derived by the “02-5B-318 × Saragolla” interspecific cross. Data refer to three environments (TKW_Val15; TKW_Val16; TKW_Val17) and the average of environments (TKW_Mean). Reported values represent the mean of three field replicates, each averaged between two measurements.

GenotypeLineLine nameTKW_Val15 (g)TKW_Val16 (g)TKW_Val17 (g)TKW_mean (g)
CommonParent02-5B-31832.6843.5630.5835.61
DurumParentSaragolla41.7651.5238.1643.81
DurumRIL (F7:F8)136.9234.7538.3036.66
DurumRIL (F7:F8)234.1821.5642.2732.67
DurumRIL (F7:F8)333.6642.9634.9737.20
DurumRIL (F7:F8)431.2327.1734.430.93
DurumRIL (F7:F8)531.6840.9646.8039.81
DurumRIL (F7:F8)637.0340.4736.4037.97
DurumRIL (F7:F8)740.4743.8440.3741.56
DurumRIL (F7:F8)839.2340.2346.3741.94
DurumRIL (F7:F8)9––––
DurumRIL (F7:F8)1042.2537.2038.2739.24
DurumRIL (F7:F8)1136.01–31.8733.94
DurumRIL (F7:F8)1239.6240.4641.3340.47
DurumRIL (F7:F8)1336.1440.2640.3738.92
DurumRIL (F7:F8)1428.2430.2432.2330.24
DurumRIL (F7:F8)1532.6930.6633.4032.25
DurumRIL (F7:F8)1637.2040.8939.8739.32
DurumRIL (F7:F8)1748.1340.8145.0344.66
DurumRIL (F7:F8)1840.4840.3445.4042.07
DurumRIL (F7:F8)1937.2142.9441.7740.64
DurumRIL (F7:F8)2042.9247.3553.0047.76
DurumRIL (F7:F8)2133.0330.7533.0332.27
DurumRIL (F7:F8)2228.0337.0528.3331.14
DurumRIL (F7:F8)2335.4640.5232.9336.30
DurumRIL (F7:F8)2445.2750.2245.2746.92
DurumRIL (F7:F8)2545.8843.8940.9743.58
DurumRIL (F7:F8)2643.2344.0036.1041.11
DurumRIL (F7:F8)2733.6440.5333.3035.82
DurumRIL (F7:F8)2836.1740.8436.3737.79
DurumRIL (F7:F8)29––––
DurumRIL (F7:F8)3037.7830.2733.2733.77
DurumRIL (F7:F8)3135.7533.8235.2034.92
DurumRIL (F7:F8)3233.2340.20–36.72
DurumRIL (F7:F8)3338.2440.6535.9738.29
DurumRIL (F7:F8)3443.1140.5139.7041.11
DurumRIL (F7:F8)3550.2247.0547.6748.31
DurumRIL (F7:F8)3636.5440.5126.4334.49
DurumRIL (F7:F8)3737.4937.1434.6736.43
DurumRIL (F7:F8)3847.58–48.2047.89
DurumRIL (F7:F8)3930.2330.5936.1732.33
DurumRIL (F7:F8)4030.8025.8336.4331.02
DurumRIL (F7:F8)4130.1730.4832.3731.01
DurumRIL (F7:F8)42––––
DurumRIL (F7:F8)4333.8430.5033.1032.48
DurumRIL (F7:F8)4426.1523.8730.3326.78
DurumRIL (F7:F8)4531.8630.6332.0731.52
DurumRIL (F7:F8)4635.5336.9937.6736.73
DurumRIL (F7:F8)4735.6933.9040.8736.82
DurumRIL (F7:F8)4840.0860.4145.4748.65
DurumRIL (F7:F8)4935.8540.8735.5737.43
DurumRIL (F7:F8)50––––
DurumRIL (F7:F8)5131.4333.2233.2332.63
DurumRIL (F7:F8)52––––
DurumRIL (F7:F8)5340.4040.3239.5740.10
DurumRIL (F7:F8)54––––
DurumRIL (F7:F8)5531.7235.7744.7337.41
DurumRIL (F7:F8)5622.5527.0534.9028.17
DurumRIL (F7:F8)5744.2240.8136.4340.49
DurumRIL (F7:F8)5834.5737.2134.7735.52
DurumRIL (F7:F8)5950.3550.3741.9047.54
DurumRIL (F7:F8)6023.7830.4733.2729.17
DurumRIL (F7:F8)6129.6330.6831.5730.63
DurumRIL (F7:F8)6222.5115.6622.7020.29
DurumRIL (F7:F8)63––––
DurumRIL (F7:F8)6433.9730.4933.9332.80
DurumRIL (F7:F8)65––––
DurumRIL (F7:F8)6631.2030.2026.1029.17
DurumRIL (F7:F8)6738.6737.1643.1039.64
DurumRIL (F7:F8)6844.7750.2040.3345.10
DurumRIL (F7:F8)6967.8450.4148.6055.62
DurumRIL (F7:F8)7039.4540.6136.5738.88
DurumRIL (F7:F8)7139.5140.5843.2741.12
DurumRIL (F7:F8)7240.5030.8236.5335.95
DurumRIL (F7:F8)7338.6540.6246.2741.85
DurumRIL (F7:F8)7437.7440.5434.6037.63
DurumRIL (F7:F8)7530.3040.3741.5337.40
DurumRIL (F7:F8)7629.5930.4931.3030.46
DurumRIL (F7:F8)7733.3730.8934.6332.96
DurumRIL (F7:F8)7836.9633.7032.0734.24
DurumRIL (F7:F8)7931.7736.9133.1033.93
DurumRIL (F7:F8)8036.9240.5639.8339.10
DurumRIL (F7:F8)8133.5730.7629.4331.25
DurumRIL (F7:F8)8228.6130.5537.7032.29
DurumRIL (F7:F8)8333.0333.8835.4334.11
DurumRIL (F7:F8)8431.6415.5028.7325.29
DurumRIL (F7:F8)85––12.1312.13
DurumRIL (F7:F8)8632.4330.599.9324.32
DurumRIL (F7:F8)8738.1537.2237.8337.73
DurumRIL (F7:F8)8830.9330.1334.4731.84
DurumRIL (F7:F8)8941.4540.2040.1040.58
DurumRIL (F7:F8)90––––
DurumRIL (F7:F8)91–40.8739.9040.39
DurumRIL (F7:F8)9223.6830.7532.8029.08
DurumRIL (F7:F8)9327.11–28.8327.97
DurumRIL (F7:F8)9434.0937.1735.7735.68
DurumRIL (F7:F8)9532.4637.1242.6037.39
DurumRIL (F7:F8)9638.9125.1132.0732.03
DurumRIL (F7:F8)9739.7450.4341.7743.98
DurumRIL (F7:F8)9835.0533.9438.3735.79
DurumRIL (F7:F8)9934.7140.4237.2737.47
DurumRIL (F7:F8)10032.71–32.1732.44
DurumRIL (F7:F8)10138.6250.2936.0341.65
DurumRIL (F7:F8)10232.2534.0033.7333.33
DurumRIL (F7:F8)10362.22––62.22
DurumRIL (F7:F8)10434.1527.0527.8029.67
DurumRIL (F7:F8)105––––
DurumRIL (F7:F8)10630.3033.8444.9336.36
DurumRIL (F7:F8)10740.4737.0944.3040.62
DurumRIL (F7:F8)108––––
DurumRIL (F7:F8)10932.8450.4446.9343.40
DurumRIL (F7:F8)11035.5037.2142.2038.30
DurumRIL (F7:F8)11123.8820.7428.7324.45
DurumRIL (F7:F8)11236.7633.9239.0336.57
DurumRIL (F7:F8)11324.9430.3635.4730.26
DurumRIL (F7:F8)11428.4830.4332.6330.51
DurumRIL (F7:F8)11528.8130.3031.5730.23
DurumRIL (F7:F8)11628.8430.4933.1330.82
DurumRIL (F7:F8)11724.3830.5829.7328.23
DurumRIL (F7:F8)11833.0233.6337.4034.68
DurumRIL (F7:F8)11939.3740.5742.2740.74
DurumRIL (F7:F8)12042.3240.5438.1340.33
DurumRIL (F7:F8)12138.4330.7539.2736.15
DurumRIL (F7:F8)12234.8340.2037.8337.62
DurumRIL (F7:F8)12335.2240.8843.8339.98
DurumRIL (F7:F8)124–20.3322.9321.63
DurumRIL (F7:F8)12535.7634.1238.9736.28
DurumRIL (F7:F8)126–40.2544.1042.18
DurumRIL (F7:F8)12735.3240.5435.7737.21
DurumRIL (F7:F8)12838.2540.3236.5738.38
DurumRIL (F7:F8)12935.7240.3634.2036.76
DurumRIL (F7:F8)13028.7433.8027.7330.09
DurumRIL (F7:F8)13131.3637.0641.6336.68
DurumRIL (F7:F8)13244.17–36.9740.57
DurumRIL (F7:F8)13325.4430.2022.8026.15
DurumRIL (F7:F8)13432.2930.8133.7332.28
DurumRIL (F7:F8)135––––
Thousand-kernel weight (TKW) in two parents and 135 durum wheat RILs derived by the “02-5B-318 × Saragolla” interspecific cross. Data refer to three environments (TKW_Val15; TKW_Val16; TKW_Val17) and the average of environments (TKW_Mean). Reported values represent the mean of three field replicates, each averaged between two measurements. Table 4 reports the composition of grain storage proteins in two parents and 135 durum RI lines obtained by the interspecific cross “02-5B-318 × Saragolla”. Parents and RILs were characterized for protein subunits encoded by the HMW-GS loci Glu-A1 and Glu-B1, and the gliadin locus Gli-B1. The common wheat parent 02-5B-318 carried the 1Ax2* allele of Glu-A1 gene encoding the 2* HMW-GS, the Bx7 and By9 alleles of Glu-B1 gene encoding 7 + 9 HMW-GS, and the allele for γ-42 gliadin at Gli-B1 locus. The durum wheat parent Saragolla had the following allele combination: null allele at the Glu-A1 locus; Bx6 and By8 alleles of Glu-B1 gene encoding 6 + 8 HMW-GS, and the allele for γ-45 gliadin at Gli-B1 locus.
Table 4

HMW-GS (Glu-A1 and Glu-B1 loci) and gliadin (Gli-B1 locus) composition of two parents and 135 durum wheat RI lines derived from the interspecific cross between the common wheat accession 02-5B-318 and the durum cv. Saragolla.

GenotypeLineLine nameGlu-A1Glu-B1Gli-B1
CommonParent02-5B-3182*7 + 9γ-42
DurumParentSaragollanull6 + 8γ-45
DurumRIL (F7:F8)1null7 + 9γ-42
DurumRIL (F7:F8)2null7 + 9γ-45
DurumRIL (F7:F8)3null7 + 9γ-45
DurumRIL (F7:F8)4null6 + 8γ-42
DurumRIL (F7:F8)5null6 + 8γ-42
DurumRIL (F7:F8)6null6 + 8γ-42
DurumRIL (F7:F8)7null6 + 8γ-42
DurumRIL (F7:F8)8null7 + 9γ-45
DurumRIL (F7:F8)9null7 + 9γ-45
DurumRIL (F7:F8)10null6 + 8γ-45
DurumRIL (F7:F8)11null7 + 9γ-45
DurumRIL (F7:F8)12null6 + 8γ-42
DurumRIL (F7:F8)13null6 + 8γ-45
DurumRIL (F7:F8)14null7 + 9γ-45
DurumRIL (F7:F8)15null7 + 9γ-42
DurumRIL (F7:F8)16null7 + 9γ-42
DurumRIL (F7:F8)17null6 + 8γ-42
DurumRIL (F7:F8)18null6 + 8γ-42
DurumRIL (F7:F8)19null7 + 9γ-45
DurumRIL (F7:F8)20null7 + 9γ-42
DurumRIL (F7:F8)21null7 + 9γ-45
DurumRIL (F7:F8)22null7 + 9γ-42
DurumRIL (F7:F8)23null7 + 9γ-45
DurumRIL (F7:F8)24null7 + 9γ-42
DurumRIL (F7:F8)25null7 + 9γ-42
DurumRIL (F7:F8)26null7 + 9γ-45
DurumRIL (F7:F8)27null6 + 8γ-42
DurumRIL (F7:F8)28null6 + 8γ-45
DurumRIL (F7:F8)29null7 + 9γ-42
DurumRIL (F7:F8)30null6 + 8γ-42
DurumRIL (F7:F8)31null6 + 8γ-45
DurumRIL (F7:F8)32null7 + 9γ-42
DurumRIL (F7:F8)33null7 + 9γ-45
DurumRIL (F7:F8)34null6 + 8γ-45
DurumRIL (F7:F8)35null6 + 8γ-42
DurumRIL (F7:F8)36null6 + 8γ-42
DurumRIL (F7:F8)37null7 + 9γ-45
DurumRIL (F7:F8)38null6 + 8γ-45
DurumRIL (F7:F8)392*6 + 8γ-42
DurumRIL (F7:F8)40null7 + 9γ-42
DurumRIL (F7:F8)41null7 + 9γ-45
DurumRIL (F7:F8)42null6 + 8γ-45
DurumRIL (F7:F8)43null7 + 9γ-45
DurumRIL (F7:F8)44null7 + 9γ-45
DurumRIL (F7:F8)45null7 + 9γ-42
DurumRIL (F7:F8)46null6 + 8γ-42
DurumRIL (F7:F8)47null6 + 8γ-42
DurumRIL (F7:F8)48null7 + 9γ-42
DurumRIL (F7:F8)49null6 + 8γ-45
DurumRIL (F7:F8)50null6 + 8γ-42
DurumRIL (F7:F8)51null7 + 9γ-45
DurumRIL (F7:F8)52null7 + 9γ-42
DurumRIL (F7:F8)53null6 + 8γ-42
DurumRIL (F7:F8)54null7 + 9γ-42
DurumRIL (F7:F8)55null6 + 8γ-45
DurumRIL (F7:F8)56null7 + 9γ-45
DurumRIL (F7:F8)57null6 + 8γ-42
DurumRIL (F7:F8)58null7 + 9γ-42
DurumRIL (F7:F8)59null7 + 9γ-42
DurumRIL (F7:F8)60null7 + 9γ-45
DurumRIL (F7:F8)61null6 + 8γ-42
DurumRIL (F7:F8)62null7 + 9γ-45
DurumRIL (F7:F8)63null6 + 8γ-45
DurumRIL (F7:F8)64null6 + 8γ-45
DurumRIL (F7:F8)65null6 + 8γ-45
DurumRIL (F7:F8)662*6 + 8γ-45
DurumRIL (F7:F8)67null6 + 8γ-45
DurumRIL (F7:F8)68null7 + 9γ-45
DurumRIL (F7:F8)69null6 + 8γ-45
DurumRIL (F7:F8)70null6 + 8γ-45
DurumRIL (F7:F8)71null6 + 8γ-45
DurumRIL (F7:F8)72null7 + 9γ-45
DurumRIL (F7:F8)73null6 + 8γ-45
DurumRIL (F7:F8)74null7 + 9γ-45
DurumRIL (F7:F8)75null7 + 9γ-45
DurumRIL (F7:F8)76null7 + 9γ-45
DurumRIL (F7:F8)77null6 + 8γ-42
DurumRIL (F7:F8)78null7 + 9γ-45
DurumRIL (F7:F8)79null6 + 8γ-42
DurumRIL (F7:F8)80null6 + 8γ-45
DurumRIL (F7:F8)81null7 + 9γ-42
DurumRIL (F7:F8)82null6 + 8γ-42
DurumRIL (F7:F8)83null6 + 8γ-45
DurumRIL (F7:F8)84null6 + 8γ-45
DurumRIL (F7:F8)85null6 + 8γ-45
DurumRIL (F7:F8)86null6 + 8γ-42
DurumRIL (F7:F8)87null7 + 9γ-45
DurumRIL (F7:F8)882*6 + 8γ-45
DurumRIL (F7:F8)89null7 + 9γ-45
DurumRIL (F7:F8)90null6 + 8γ-45
DurumRIL (F7:F8)91null7 + 9γ-42
DurumRIL (F7:F8)92null7 + 9γ-45
DurumRIL (F7:F8)93null7 + 9γ-42
DurumRIL (F7:F8)94null7 + 9γ-45
DurumRIL (F7:F8)95null7 + 9γ-42
DurumRIL (F7:F8)96null7 + 9γ-45
DurumRIL (F7:F8)97null6 + 8γ-45
DurumRIL (F7:F8)98null6 + 8γ-45
DurumRIL (F7:F8)99null6 + 8γ-45
DurumRIL (F7:F8)100null6 + 8γ-42
DurumRIL (F7:F8)101null7 + 9γ-45
DurumRIL (F7:F8)102null7 + 9γ-45
DurumRIL (F7:F8)103null6 + 8γ-42
DurumRIL (F7:F8)104null6 + 8γ-42
DurumRIL (F7:F8)105null7 + 9γ-45
DurumRIL (F7:F8)106null6 + 8γ-45
DurumRIL (F7:F8)107null6 + 8γ-45
DurumRIL (F7:F8)108null6 + 8γ-42
DurumRIL (F7:F8)109null6 + 8γ-45
DurumRIL (F7:F8)110null6 + 8γ-42
DurumRIL (F7:F8)111null6 + 8γ-45
DurumRIL (F7:F8)112null6 + 8γ-45
DurumRIL (F7:F8)113null6 + 8γ-45
DurumRIL (F7:F8)114null6 + 8γ-45
DurumRIL (F7:F8)115null6 + 8γ-45
DurumRIL (F7:F8)116null7 + 9γ-45
DurumRIL (F7:F8)117null6 + 8γ-42
DurumRIL (F7:F8)118null6 + 8γ-45
DurumRIL (F7:F8)119null7 + 9γ-42
DurumRIL (F7:F8)120null7 + 9γ-45
DurumRIL (F7:F8)121null7 + 9γ-45
DurumRIL (F7:F8)122null7 + 9γ-42
DurumRIL (F7:F8)123null6 + 8γ-42
DurumRIL (F7:F8)124null7 + 9γ-45
DurumRIL (F7:F8)125null6 + 8γ-42
DurumRIL (F7:F8)126null7 + 9γ-45
DurumRIL (F7:F8)127null6 + 8γ-45
DurumRIL (F7:F8)128null6 + 8γ-42
DurumRIL (F7:F8)129null6 + 8γ-42
DurumRIL (F7:F8)130null6 + 8γ-45
DurumRIL (F7:F8)131null7 + 9γ-45
DurumRIL (F7:F8)132null7 + 9γ-42
DurumRIL (F7:F8)133null6 + 8γ-45
DurumRIL (F7:F8)134null7 + 9γ-45
DurumRIL (F7:F8)135null6 + 8γ-45
HMW-GS (Glu-A1 and Glu-B1 loci) and gliadin (Gli-B1 locus) composition of two parents and 135 durum wheat RI lines derived from the interspecific cross between the common wheat accession 02-5B-318 and the durum cv. Saragolla. Fig. 1 depicts SDS-PAGE analysis of seed extracts from the two parents and some durum RILs obtained by the interspecific cross “02-5B-318 × Saragolla”. In the photo, wheat lines were screened for the HMW glutenin subunits encoded by Glu-A1 and Glu-B1 loci and were classified for carrying the allele combination of 02-5B-318 parent (2*/ 7 + 9 HMW-GS) or Saragolla (null 2*/ 6 + 8 HMW-GS). Testers included in the analyses were the common wheat cv Chinese Spring and three hexaploid lines (Tester 1, 2, 3).
Fig. 1

SDS-PAGE of seed extracts from the two parents and some durum RILs of “02-5B-318 × Saragolla” population. RILs were screened for the HMW glutenin subunits encoded by Glu-A1 (2*/null) and Glu-B1 (7 + 9/6 + 8) loci. Common wheat cv Chinese Spring and other hexaploid testers (1, 2, 3) are also reported as controls.

SDS-PAGE of seed extracts from the two parents and some durum RILs of “02-5B-318 × Saragolla” population. RILs were screened for the HMW glutenin subunits encoded by Glu-A1 (2*/null) and Glu-B1 (7 + 9/6 + 8) loci. Common wheat cv Chinese Spring and other hexaploid testers (1, 2, 3) are also reported as controls. Colour use is required for Fig. 1 in print. Supplementary Table 1 lists SNP markers located in the confidence intervals of mapped QTLs for GPC, which were searched for a putative function related to wheat seed storage proteins quality and quantity (protein synthesis/accumulation). Each SNP is annotated with the corresponding marker name, identification code, SNP type, nucleotide sequence/length, and chromosomal position on the durum wheat genetic linkage map developed by Giancaspro et al. [2]. Supplementary Table 2 lists SNP markers located in the confidence intervals of mapped QTLs for GYS, which were searched for a putative function related to wheat productivity (grain yield components). Each SNP is annotated with the corresponding marker name, identification code, SNP type, nucleotide sequence/length and chromosomal position on the durum wheat genetic linkage map developed by Giancaspro et al. [2]. Supplementary Table 3 lists SNP markers located in the confidence intervals of mapped QTLs for TKW, which were searched for a putative function related to wheat productivity (grain yield components). Each SNP is annotated with the corresponding marker name, identification code, SNP type, nucleotide sequence/length and chromosomal position on the durum wheat linkage genetic map developed by Giancaspro et al. [2].

Experimental Design, Materials and Methods

Data reported in this article describe the genetic variability for quantity (grain protein content, GPC) and composition (HMW-GS, gliadins) of seed storage proteins, and two yield components - grain yield per spike (GYS) and thousand kernel weight (TKW) - in 135 lines of a durum wheat RIL population obtained by crossing the common wheat accession 02-5B-318 (derived from the Chinese cv. Sumai-3) and the élite durum cv. Saragolla, and advancing single F2 plants to the F7 by single seed descent (SSD) [1,2,[7], [8], [9], [10]]. GYS, TKW and GPC data were collected from plants grown in replicated trials in the fields of Valenzano (Metropolitan City of Bari, BA, Italy) for three years (2015, 2016, 2017) using a randomized block design with three replications. Each plot consisted of 1 m rows, 30 cm apart, with 80 seeds sown in each plot and supplemented with 10 g/m2 nitrogen. GYS was calculated by dividing the total grain yield per row on the number of spikes per row (about 70–80 spikes). TKW was acquired from a 15 g seed sample per each line, as the average of two technical replicates. GPC was measured by NIR spectroscopy on a 2 g sample of whole-meal flour using a SpectraAlyzer device. The instrument was calibrated with a set of 25 whole-meal flour samples of T. turgidum ssp. durum, ssp. dicoccum, and ssp. dicoccoides with known protein concentration and moisture, previously calculated according to official standard methods. For each wheat line, GPC was expressed as the percentage of proteins on a dry weight basis, averaged between two technical replicates. In detail, protein content on dry matter (GPC% d.m.) was calculated with the following formula: [Tot. GPC% / (100 − H%)] × 100. Parents and durum RILs were characterized for protein subunits encoded by the HMW-GS loci Glu-A1 and Glu-B1 and the gliadin locus Gli-B1, which are crucial for bread-making quality. Gliadins were extracted from 30 μg of single-seed flour by following the protocol by Payne et al. [3]. HMW-glutenin subunits were determined on grain extracts according to the method of Bushuk and Zillman [4]. Protein subunits were identified based on their relative mobility in SDS-PAGE vertical electrophoresis, and HMW-GS composition was scored according to Payne's catalogue [11]. Progenies were classified for carrying alleles coming from 02-5B-318 or Saragolla. The common wheat parent 02-5B-318 carried the 1Ax2* allele of Glu-A1 gene encoding the 2* HMW-GS, the Bx7 and By9 alleles of Glu-B1 gene encoding 7 + 9 HMW-GS, and the allele for γ-42 gliadin at Gli-B1 locus. The durum wheat parent Saragolla had the following alleles combination: non-coding Glu-A1 locus; Bx6 and By8 alleles of Glu-B1 gene encoding 6 + 8 HMW-GS, and the allele for γ-45 gliadin at Gli-B1 locus. Gel imaging acquisition and software analyses were performed by DigiDoc (Bio-Rad Laboratories). A durum wheat SNP-based genetic linkage map developed by Giancaspro et al. [2] was used for QTL detection. The high-density map consisted of 4,366 SNPs surveyed from the 81,587 sequences of the 90 K iSelect array by Illumina CSProR (San Diego, CA, USA) described by Wang et al. [12]. ANOVA was conducted for GYS, TKW and GPC traits with standard procedures using X-Stat software [13], and genetic variance (σ2G) and broad-sense heritability (h2B) evaluated using the variance component estimates. Pearson phenotypic correlation coefficients were calculated between GPC, GYS and TKW by using MSTAT-C statistical software [14]. QTL mapping was performed according to the Inclusive Composite Interval Mapping (ICIM) method [5] implemented in QGene 4.0 software [6]. Scanning interval of 2 cM between markers, and putative QTL with a window size of 10 cM were used as criteria for QTLs detection. The number of marker cofactors for background control was set by forward regression with a maximum of five controlling markers. Putative QTLs were defined as two or more trait-associated markers at LOD ≥ 3. Trait-associated SNPs at LOD ≤ 2 were considered not significant. Suggestive QTLs were classified as one or more trait-associated SNP markers at the subthreshold of 2.0 < LOD < 3.0. The following criteria were used for classification of additive effects: positive additive effects were associated with increased trait values from Saragolla alleles, whereas negative additive effects were contributed by 02-5B-318 alleles. For the identification of candidate genes, sequences of SNP markers mapping in the confidence intervals of QTLs for GPC, GYS and TKW were blasted using TBLASTX algorithm (http://ncbi.nlm.nih.gov/BLAST) against the wheat draft genome sequence of the tetraploid dicoccoides accession Zavitan [15], the durum cv. Svevo [16], and the hexaploid cv. Chinese Spring [17]. Only alignments scoring ≥80% identity, >10−7 E-value and sequences involved in metabolic pathways related to protein content and yield components were considered as putative candidate genes.

Ethics Statements

No ethics statement.

CRediT authorship contribution statement

Angelica Giancaspro: Methodology, Investigation, Formal analysis, Visualization, Writing – original draft. Stefania Lucia Giove: Investigation, Validation, Data curation. Ilaria Marcotuli: Validation. Agata Gadaleta: Conceptualization, Methodology, Resources, Project administration, Funding acquisition, Supervision, Writing – review & editing.

Declaration of Competing Interest

The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
SubjectAgricultural Sciences - Agronomy and Crop Science
Specific subject areaThe subject area deals with the evaluation of agronomic traits of crop species (durum wheat) including quality, quantity, and productivity. The specific area also includes molecular studies, as investigating genetic loci (QTLs and candidate genes) controlling the traits of interest.
Type of dataTable Figure
How the data were acquiredData on wheat quality and productivity described in this article refer to 135 durum wheat RI lines obtained by the interspecific cross “02-5B-318 × Saragolla”, grown in replicated field trials at the location of Valenzano (Metropolitan City of Bari, BA, Italy) for three years (2015, 2016, 2017) [2].Data on grain protein content (GPC) were acquired by Near-Infrared Reflectance (NIR) spectroscopy on a 2-g sample of whole-meal flour using a SpectraAlyzer device (Basic model, Zeuton). For each wheat line, GPC was expressed as the percentage of proteins on a dry weight basis, averaged between two technical replicates. Data on grain yield per spike (GYS) were obtained by dividing the total grain yield per row on the number of spikes per row (about 70-80 spikes). Data on thousand-kernel weight (TKW) were derived from a 15-gram seed sample per each line, as the average of two technical replicates.Data on grain storage proteins composition were acquired from SDS-PAGE electrophoresis of wheat seed extracts. Gliadins and HMW-glutenin subunits were extracted from whole-grain samples according to the protocols by Payne et al. [3] and Bushuk and Zillman [4], respectively. Protein identification was based on relative mobility in SDS-electrophoresis run in Protean II Xi vertical cells (Bio-Rad Laboratories); gel imaging acquisition and software analysis were performed by DigiDoc (Bio-Rad Laboratories).QTL detection was performed using the Inclusive Composite Interval Mapping (ICIM) method [5] implemented in QGene 4.0 software [6]. Genotypic data for QTL mapping were acquired from the 4,366 SNP-based durum wheat genetic linkage map developed by Giancaspro et al. [2].Putative functions of SNP markers located in the QTLs confidence intervals for GPC, GYS and TKW were acquired by a bioinformatic survey: SNP sequences were blasted against draft genomes of tetraploid and hexaploid wheat cultivars by using the TBLASTX algorithm at: http://ncbi.nlm.nih.gov/BLAST. Only matches scoring ≥ 80% identity and >10−7 E-value were considered for the identification of candidate genes.
Data formatAnalysed Filtered
Description of data collectionData described in this article refer to 135 durum wheat recombinant inbred lines obtained by an interspecific cross between the bread wheat accession 02-5B-318 (derived from the Chinese cv. Sumai-3) and the élite durum wheat cv. Saragolla [2]. The two crossing parents were chosen because different for several agronomic traits related to grain quality (grain protein content, yellow index, gluten index, plant height, spike compactness), productivity (yield components), and resistance to Fusarium Head Blight (FHB-resistant 02-5B-318, FHB-susceptible Saragolla). The same population was previously employed for several aims: development of a durum wheat genetic linkage map [2], identification of cell wall traits conferring resistance to FHB [7,8], mapping of QTLs for FHB resistance, quality- and yield-related traits [1,2,9], map-based cloning of FHB resistance genes [10].Datasets in this paper only refer to the “02-5B-318 × Saragolla” progenies lacking D-genome chromosomes (tetraploid subset), whereas all wheat RILs carrying D-genome sequences (hexaploid subset) were excluded from the analyses.Data on GPC, GYS and TKW of parents and durum RILs were collected from plants grown in replicated field trials at the location of Valenzano (Metropolitan City of Bari, BA, Italy) for three years (from 2015 to 2017) by using a randomized complete block design with three replicates.QTL detection was performed by QGene software [6] using the ICIM method [5]: a LOD threshold ≥ 3 was set for the identification of putative QTLs. Trait-associated SNPs at LOD ≤ 2 were considered not significant. Suggestive QTLs were classified as one or more trait-associated SNP marker at the subthreshold of 2.0 < LOD < 3.0. The following criteria were used for classification of additive effects: positive additive effects were associated with increased trait values from Saragolla alleles, whereas negative additive effects were contributed from 02-5B-318 alleles.Parents and durum RILs were characterized for seed storage protein subunits encoded by the HMW-GS loci Glu-A1 and Glu-B1, and the gliadin locus Gli-B1. “02-5B-318 × Saragolla” progenies were scored for carrying the alleles coming from 02-5B-318 parent (2* HMW-GS, 7 + 9 HMW-GS, γ-42 gliadin) or Saragolla parent (null 2* HMW-GS, 6 + 8 HMW-GS, γ-45 gliadin). Data on protein composition were acquired based on their relative mobility in vertical SDS-PAGE.For the identification of candidate genes among SNPs mapping in the QTLs confidence intervals for GPC, GYS and TKW, only sequence alignments scoring ≥ 80% identity and >10−7 E-value were considered, and the corresponding putative genes evaluated for their actual involvement in metabolic pathways related to wheat protein quality/content and productivity. Matches with < 80% identity were not considered.
Data source locationField data collection:

Azienda Agricola “Martucci”

Valenzano (Metropolitan City of Bari, BA)

Italy

Lat. 41.0438° N, long. 16.8842° E, elevation 85 m above sea level.

Secondary data production (analyses and filtering):

University of Bari “Aldo Moro”, Department of Agricultural and Environmental Science (DiSAAT)

Metropolitan City of Bari (BA)

Italy

Lat. 41.12688° N, long. 16.86596° E, elevation 5 m above sea level.

Data accessibilityRepository name: Mendeley Data Data identification number: DOI:10.17632/g6gsfn9k9j.1 Direct URL to data: https://data.mendeley.com/datasets/g6gsfn9k9j/1
Related research articleA. Giancaspro, S.L. Giove, S.A. Zacheo, A. Blanco, A. Gadaleta, Genetic variation for protein content and yield-related traits in a durum population derived from an inter-specific cross between hexaploid and tetraploid wheat cultivars, Front. Plant Sci. 10 (2019) 1509.
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