Literature DB >> 35524051

Engineering Modular Polyketide Biosynthesis in Streptomyces Using CRISPR/Cas: A Practical Guide.

Jean-Malo Massicard1, Li Su1, Christophe Jacob2, Kira J Weissman3.   

Abstract

The CRISPR/Cas system, which has been widely applied to organisms ranging from microbes to animals, is currently being adapted for use in Streptomyces bacteria. In this case, it is notably applied to rationally modify the biosynthetic pathways giving rise to the polyketide natural products, which are heavily exploited in the medical and agricultural arenas. Our aim here is to provide the potential user with a practical guide to exploit this approach for manipulating polyketide biosynthesis, by treating key experimental aspects including vector choice, design of the basic engineering components, and trouble-shooting.
© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Entities:  

Keywords:  CRISPR/Cas; Genome editing; Polyketide biosynthesis; Streptomyces

Mesh：

Substances：

Year:  2022        PMID： 35524051     DOI： 10.1007/978-1-0716-2273-5_10

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  38 in total

1.  CRISPR-Cas9 Based Engineering of Actinomycetal Genomes.

Authors:  Yaojun Tong; Pep Charusanti; Lixin Zhang; Tilmann Weber; Sang Yup Lee
Journal:  ACS Synth Biol       Date:  2015-04-07       Impact factor: 5.110

Review 2.  Bacterial NHEJ: a never ending story.

Authors:  Claire Bertrand; Annabelle Thibessard; Claude Bruand; François Lecointe; Pierre Leblond
Journal:  Mol Microbiol       Date:  2019-03-18       Impact factor: 3.501

Review 3.  CRISPR/Cas-based genome engineering in natural product discovery.

Authors:  Yaojun Tong; Tilmann Weber; Sang Yup Lee
Journal:  Nat Prod Rep       Date:  2019-09-18       Impact factor: 13.423

Review 4.  Next Generation Prokaryotic Engineering: The CRISPR-Cas Toolkit.

Authors:  Ioannis Mougiakos; Elleke F Bosma; Willem M de Vos; Richard van Kranenburg; John van der Oost
Journal:  Trends Biotechnol       Date:  2016-03-02       Impact factor: 19.536

Review 5.  Regulation of antibiotic production in Actinobacteria: new perspectives from the post-genomic era.

Authors:  Helga U van der Heul; Bohdan L Bilyk; Kenneth J McDowall; Ryan F Seipke; Gilles P van Wezel
Journal:  Nat Prod Rep       Date:  2018-06-20       Impact factor: 13.423

6.  A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity.

Authors:  Martin Jinek; Krzysztof Chylinski; Ines Fonfara; Michael Hauer; Jennifer A Doudna; Emmanuelle Charpentier
Journal:  Science       Date:  2012-06-28       Impact factor: 47.728

Review 7.  CRISPR-Cas systems for editing, regulating and targeting genomes.

Authors:  Jeffry D Sander; J Keith Joung
Journal:  Nat Biotechnol       Date:  2014-03-02       Impact factor: 54.908

Review 8.  Editing streptomycete genomes in the CRISPR/Cas9 age.

Authors:  Fabrizio Alberti; Christophe Corre
Journal:  Nat Prod Rep       Date:  2019-09-18       Impact factor: 13.423

9.  High-efficiency multiplex genome editing of Streptomyces species using an engineered CRISPR/Cas system.

Authors:  Ryan E Cobb; Yajie Wang; Huimin Zhao
Journal:  ACS Synth Biol       Date:  2014-12-08       Impact factor: 5.110

10.  Diversity oriented biosynthesis via accelerated evolution of modular gene clusters.

Authors:  Aleksandra Wlodek; Steve G Kendrew; Nigel J Coates; Adam Hold; Joanna Pogwizd; Steven Rudder; Lesley S Sheehan; Sarah J Higginbotham; Anna E Stanley-Smith; Tony Warneck; Mohammad Nur-E-Alam; Markus Radzom; Christine J Martin; Lois Overvoorde; Markiyan Samborskyy; Silke Alt; Daniel Heine; Guy T Carter; Edmund I Graziani; Frank E Koehn; Leonard McDonald; Alexander Alanine; Rosa María Rodríguez Sarmiento; Suzan Keen Chao; Hasane Ratni; Lucinda Steward; Isobel H Norville; Mitali Sarkar-Tyson; Steven J Moss; Peter F Leadlay; Barrie Wilkinson; Matthew A Gregory
Journal:  Nat Commun       Date:  2017-10-31       Impact factor: 17.694
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.