Literature DB >> 3546359

Indirect immunofluorescence assay for antibodies to human immunodeficiency virus.

E T Lennette, S Karpatkin, J A Levy.   

Abstract

We compared an indirect immunofluorescence assay (IFA) with a commercial enzyme-linked immunosorbent assay (ELISA) screening procedure for the detection of antibodies to the human immunodeficiency virus (HIV). The IFA assay was as sensitive and specific as the ELISA. No false results were observed with IFA on single testing of 181 sera, while the ELISA produced five false-positive results which required retesting for resolution. In addition, the IFA was suitable for quantitation of anti-HIV responses. The IFA was superior to the ELISA for its reliability, simplicity, and rapidity in the diagnostic laboratory.

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Year:  1987        PMID： 3546359      PMCID： PMC265866          DOI： 10.1128/jcm.25.2.199-202.1987

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  4 in total

1.  Asymptomatic blood donor with a false positive HTLV-III Western blot.

Authors:  M S Saag; J Britz
Journal:  N Engl J Med       Date:  1986-01-09       Impact factor: 91.245

2.  Screening test for HTLV-III (AIDS agent) antibodies. Specificity, sensitivity, and applications.

Authors:  S H Weiss; J J Goedert; M G Sarngadharan; A J Bodner; R C Gallo; W A Blattner
Journal:  JAMA       Date:  1985-01-11       Impact factor: 56.272

3.  Comparison of detection of antibody to the acquired immune deficiency syndrome virus by enzyme immunoassay, immunofluorescence, and Western blot methods.

Authors:  D Gallo; J L Diggs; G R Shell; P J Dailey; M N Hoffman; J L Riggs
Journal:  J Clin Microbiol       Date:  1986-06       Impact factor: 5.948

4.  High prevalence of antibodies to acquired immune deficiency syndrome (AIDS)-associated retrovirus (ARV) in AIDS and related conditions but not in other disease states.

Authors:  L S Kaminsky; T McHugh; D Stites; P Volberding; G Henle; W Henle; J A Levy
Journal:  Proc Natl Acad Sci U S A       Date:  1985-08       Impact factor: 11.205
  4 in total
  14 in total

1.  Evaluation of an indirect immunofluorescence assay for confirmation of human immunodeficiency virus type 1 antibody in U.S. blood donor sera.

Authors:  M T Sullivan; H Mucke; S D Kadey; C T Fang; A E Williams
Journal:  J Clin Microbiol       Date:  1992-09       Impact factor: 5.948

2.  Indirect immunofluorescence test performance and questionnaire results from the Centers for Disease Control Model Performance Evaluation Program for human immunodeficiency virus type 1 testing.

Authors:  R N Taylor; T L Hearn; W O Schalla; R O Valdiserri
Journal:  J Clin Microbiol       Date:  1990-08       Impact factor: 5.948

3.  Humoral immune response to the bovine immunodeficiency-like virus in experimentally and naturally infected cattle.

Authors:  C A Whetstone; M J VanDerMaaten; J W Black
Journal:  J Virol       Date:  1990-07       Impact factor: 5.103

4.  Sensitivity and specificity of pooled versus individual sera in a human immunodeficiency virus antibody prevalence study.

Authors:  B Cahoon-Young; A Chandler; T Livermore; J Gaudino; R Benjamin
Journal:  J Clin Microbiol       Date:  1989-08       Impact factor: 5.948

5.  Reliable confirmation of antibodies to human immunodeficiency virus type 1 (HIV-1) with an enzyme-linked immunoassay using recombinant antigens derived from the HIV-1 gag, pol, and env genes.

Authors:  V L Ng; C S Chiang; C Debouck; M S McGrath; T H Grove; J Mills
Journal:  J Clin Microbiol       Date:  1989-05       Impact factor: 5.948

6.  SILAC Based Proteomic Characterization of Exosomes from HIV-1 Infected Cells.

Authors:  Collins Cheruiyot; Zemplen Pataki; Robert Williams; Bharat Ramratnam; Ming Li
Journal:  J Vis Exp       Date:  2017-03-03       Impact factor: 1.355

7.  Immunofluorescence assay for human immunodeficiency virus antibody: investigation of cell fixation for virus inactivation and antigen preservation.

Authors:  M Fauvel; G Ozanne
Journal:  J Clin Microbiol       Date:  1989-08       Impact factor: 5.948

8.  Human herpesvirus 6 inhibits human immunodeficiency virus type 1 replication in cell culture.

Authors:  J A Levy; A Landay; E T Lennette
Journal:  J Clin Microbiol       Date:  1990-10       Impact factor: 5.948

9.  Agreement study between two laboratories of immunofluorescence as a confirmatory test for human immunodeficiency virus type 1 antibody screening.

Authors:  J Mahony; K Rosenthal; M Chernesky; S Castriciano; E Scheid; M Blajchman; D Harnish
Journal:  J Clin Microbiol       Date:  1989-06       Impact factor: 5.948

10.  Advantages of a human immunodeficiency virus type 1 (HIV-1) persistently infected HeLa T4+ cell line for HIV-1 indirect immunofluorescence serology.

Authors:  B Forghani; J W Hurst; C S Chan
Journal:  J Clin Microbiol       Date:  1991-10       Impact factor: 5.948
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