Literature DB >> 2306211

The purification and characterization of 3-dehydroquinase from Streptomyces coelicolor.

P J White1, J Young, I S Hunter, H G Nimmo, J R Coggins.   

Abstract

The enzyme 3-dehydroquinase was purified over 4000-fold to homogeneity from Streptomyces coelicolor. The subunit Mr estimated from polyacrylamide-gel electrophoresis in the presence of SDS was 16,000. The native Mr estimated by gel filtration on a Superose 6 column was 209,000, indicating that the enzyme is a large oligomer. The enzyme was found to be extremely thermostable. This stability, along with the structural and kinetic properties of the enzyme, suggest that it is very similar to the quinate-inducible 3-dehydroquinase found in Neurospora crassa and Aspergillus nidulans. This similarity was confirmed by direct N-terminal sequencing.

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Year:  1990        PMID： 2306211      PMCID： PMC1133695          DOI： 10.1042/bj2650735

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  16 in total

1.  Isolation and partial characterization of the glutamate/aspartate transporter from pea leaf mitochondria using a specific monoclonal antibody.

Authors:  J Vivekananda; D J Oliver
Journal:  Plant Physiol       Date:  1989-09       Impact factor: 8.340

Review 2.  Gene organization and regulation in the qa (quinic acid) gene cluster of Neurospora crassa.

Authors:  N H Giles; M E Case; J Baum; R Geever; L Huiet; V Patel; B Tyler
Journal:  Microbiol Rev       Date:  1985-09

3.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

4.  The overexpression and complete amino acid sequence of Escherichia coli 3-dehydroquinase.

Authors:  K Duncan; S Chaudhuri; M S Campbell; J R Coggins
Journal:  Biochem J       Date:  1986-09-01       Impact factor: 3.857

5.  Experimentally improved reliability of ultrasensitive silver staining of protein in polyacrylamide gels.

Authors:  M Eschenbruch; R R Bürk
Journal:  Anal Biochem       Date:  1982-09-01       Impact factor: 3.365

6.  Purification and characterization of 3-dehydroquinate hydrolase and shikmate oxidoreductase. Evidence for a bifunctional enzyme.

Authors:  L D Polley
Journal:  Biochim Biophys Acta       Date:  1978-09-11

7.  The isolation and nucleotide sequence of the complex AROM locus of Aspergillus nidulans.

Authors:  I G Charles; J W Keyte; W J Brammar; M Smith; A R Hawkins
Journal:  Nucleic Acids Res       Date:  1986-03-11       Impact factor: 16.971

8.  Organization of enzymes in the polyaromatic synthetic pathway: separability in bacteria.

Authors:  M B Berlyn; N H Giles
Journal:  J Bacteriol       Date:  1969-07       Impact factor: 3.490

9.  Molecular organisation of the quinic acid utilization (QUT) gene cluster in Aspergillus nidulans.

Authors:  A R Hawkins; H K Lamb; M Smith; J W Keyte; C F Roberts
Journal:  Mol Gen Genet       Date:  1988-10

10.  Sequence analysis and transformation by the catabolic 3-dehydroquinase (QUTE) gene from Aspergillus nidulans.

Authors:  A J Da Silva; H Whittington; J Clements; C Roberts; A R Hawkins
Journal:  Biochem J       Date:  1986-12-01       Impact factor: 3.857
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  14 in total

1.  A comparison of the enzymological and biophysical properties of two distinct classes of dehydroquinase enzymes.

Authors:  C Kleanthous; R Deka; K Davis; S M Kelly; A Cooper; S E Harding; N C Price; A R Hawkins; J R Coggins
Journal:  Biochem J       Date:  1992-03-15       Impact factor: 3.857

2.  The folding and assembly of the dodecameric type II dehydroquinases.

Authors:  N C Price; D J Boam; S M Kelly; D Duncan; T Krell; D G Gourley; J R Coggins; R Virden; A R Hawkins
Journal:  Biochem J       Date:  1999-02-15       Impact factor: 3.857

3.  Conformational changes and the role of metals in the mechanism of type II dehydroquinase from Aspergillus nidulans.

Authors:  J R Bottomley; A R Hawkins; C Kleanthous
Journal:  Biochem J       Date:  1996-10-01       Impact factor: 3.857

4.  Insights into the mechanism of type I dehydroquinate dehydratases from structures of reaction intermediates.

Authors:  Samuel H Light; George Minasov; Ludmilla Shuvalova; Mark-Eugene Duban; Michael Caffrey; Wayne F Anderson; Arnon Lavie
Journal:  J Biol Chem       Date:  2010-11-18       Impact factor: 5.157

5.  Characterization of the type I dehydroquinase from Salmonella typhi.

Authors:  J D Moore; A R Hawkins; I G Charles; R Deka; J R Coggins; A Cooper; S M Kelly; N C Price
Journal:  Biochem J       Date:  1993-10-01       Impact factor: 3.857

6.  Phosphoglycerate mutase from Streptomyces coelicolor A3(2): purification and characterization of the enzyme and cloning and sequence analysis of the gene.

Authors:  P J White; J Nairn; N C Price; H G Nimmo; J R Coggins; I S Hunter
Journal:  J Bacteriol       Date:  1992-01       Impact factor: 3.490

7.  Inducible overproduction of the Aspergillus nidulans pentafunctional AROM protein and the type-I and -II 3-dehydroquinases from Salmonella typhi and Mycobacterium tuberculosis.

Authors:  J D Moore; H K Lamb; T Garbe; S Servos; G Dougan; I G Charles; A R Hawkins
Journal:  Biochem J       Date:  1992-10-01       Impact factor: 3.857

8.  Characterization of the 3-dehydroquinase domain of the pentafunctional AROM protein, and the quinate dehydrogenase from Aspergillus nidulans, and the overproduction of the type II 3-dehydroquinase from neurospora crassa.

Authors:  A R Hawkins; J D Moore; A M Adeokun
Journal:  Biochem J       Date:  1993-12-01       Impact factor: 3.857

9.  Regulation of expression of genes involved in quinate and shikimate utilization in Corynebacterium glutamicum.

Authors:  Haruhiko Teramoto; Masayuki Inui; Hideaki Yukawa
Journal:  Appl Environ Microbiol       Date:  2009-04-17       Impact factor: 4.792

10.  The Mycobacterium tuberculosis shikimate pathway genes: evolutionary relationship between biosynthetic and catabolic 3-dehydroquinases.

Authors:  T Garbe; S Servos; A Hawkins; G Dimitriadis; D Young; G Dougan; I Charles
Journal:  Mol Gen Genet       Date:  1991-09
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