| Literature DB >> 35218541 |
Frank L Jalufka1, Sun Won Min1, Madison E Platt1, Anna L Pritchard1, Theodore E Margo1, Alexandra O Vernino1, Megan A Kirchhoff1, Ryan T Massopust2, Dylan A McCreedy3,4.
Abstract
Optical tissue clearing enables the precise imaging of cellular and subcellular structures in whole organs and tissues without the need for physical tissue sectioning. By combining tissue clearing with confocal or lightsheet microscopy, 3D images can be generated of entire specimens for visualization and large-scale data analysis. Here we demonstrate two different passive tissue clearing techniques that are compatible with immunofluorescent staining and lightsheet microscopy: PACT, an aqueous hydrogel-based clearing method, and iDISCO+, an organic solvent-based clearing method.Entities:
Keywords: Lightsheet microscopy; PACT; Passive CLARITY technique; Tissue clearing; iDISCO+
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Year: 2022 PMID： 35218541 DOI： 10.1007/978-1-0716-2051-9_12
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745