| Literature DB >> 27238021 |
Nicolas Renier1, Eliza L Adams1, Christoph Kirst2, Zhuhao Wu1, Ricardo Azevedo1, Johannes Kohl3, Anita E Autry3, Lolahon Kadiri4, Kannan Umadevi Venkataraju5, Yu Zhou6, Victoria X Wang6, Cheuk Y Tang6, Olav Olsen1, Catherine Dulac3, Pavel Osten7, Marc Tessier-Lavigne8.
Abstract
Understanding how neural information is processed in physiological and pathological states would benefit from precise detection, localization, and quantification of the activity of all neurons across the entire brain, which has not, to date, been achieved in the mammalian brain. We introduce a pipeline for high-speed acquisition of brain activity at cellular resolution through profiling immediate early gene expression using immunostaining and light-sheet fluorescence imaging, followed by automated mapping and analysis of activity by an open-source software program we term ClearMap. We validate the pipeline first by analysis of brain regions activated in response to haloperidol. Next, we report new cortical regions downstream of whisker-evoked sensory processing during active exploration. Last, we combine activity mapping with axon tracing to uncover new brain regions differentially activated during parenting behavior. This pipeline is widely applicable to different experimental paradigms, including animal species for which transgenic activity reporters are not readily available.Entities:
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Year: 2016 PMID： 27238021 PMCID： PMC4912438 DOI： 10.1016/j.cell.2016.05.007
Source DB: PubMed Journal: Cell ISSN： 0092-8674 Impact factor: 41.582