Relationship Between the Triglyceride Glucose Index and the Risk of First Stroke in Elderly Hypertensive Patients.

BACKGROUND: Several recent studies have shown the relationship between the triglyceride glucose (TyG) index and the risk of stroke in the general population and in a few patient cohorts; however, the role of the TyG index on stroke risk in elderly hypertensive patients has not been determined. Thus, we aimed to investigate the association of the TyG index with first stroke and first ischemic stroke in elderly individuals with hypertension.
METHODS: We included 8487 elderly subjects with hypertension from the China H-type Hypertension Registry Study for the current analysis. The TyG index was calculated as ln (fasting triglyceride [mg/dL] × fasting glucose [mg/dL]/2). Outcomes were the first stroke and first ischemic stroke.
RESULTS: During a median follow-up of 1.72 years, the first stroke was diagnosed in 82 patients (0.97%), and the first ischemic stroke was diagnosed in 48 patients (0.57%). Multivariable Cox proportional hazards models revealed that the TyG index was positively associated with the risk of first stroke (per 1-unit increment; HR: 1.72; 95% CI: 1.07, 2.76) and first ischemic stroke (HR: 2.31; 95% CI: 1.32, 4.05). When the TyG index was assessed as quartiles, significantly higher risks of first stroke (HR: 1.90; 95% CI: 1.04, 3.45) and first ischemic stroke (HR: 2.45; 95% CI: 1.16, 5.20) were found in participants in quartile 4 compared with those in quartiles 1-3.
CONCLUSION: The TyG index is potentially useful in the early identification of elderly hypertensive patients at high risk of experiencing a first stroke.

Introduction

Stroke is a leading cause of morbidity and mortality worldwide1 and is of increasing concern in China.2 A recent national epidemiologic survey indicated that there are 2.4 million new stroke cases annually and 1.1 million stroke-related deaths in China.3 Among the pathological subtypes of stroke, ischemic stroke is the most common subtype, accounting for approximately 77.8% of the prevalent strokes.4 Current studies have shown that high blood pressure is the leading risk factor for the global disease burden, in particular, the risk of stroke.5,6 Additionally, being older and ageing are also associated with an increased risk of stroke but also with a rising blood pressure and a higher likelihood of suffering from hypertension.7,8 It has been reported that 77% of elderly patients with hypertension experience stroke.9 Given the ageing trend in the next decades, along with the increase in hypertension prevalence,8 there is a need to identify the population at high risk of stroke in elderly hypertensive patients and to deliver effective secondary prevention.

Insulin resistance (IR), a precursor and mechanism of type 2 diabetes, which has a strong relationship with atherosclerosis,10 diabetes,11 chronic kidney disease,12 and cancer.13 Accumulating evidence has also suggested that IR may have an important impact on the risk of stroke.10,14,15 The triglyceride-glucose (TyG) index, which is calculated by fasting glucose and triglycerides, has been reported to be significantly correlated with the hyperinsulinaemic euglycaemic clamp test and to be a reliable surrogate marker of IR.16 Several recent studies have shown an association between the TyG index and stroke in the general population.17–22 However, to our knowledge, the role of TyG index has not been examined in such a high-risk hypertensive older adult population. Therefore, this study aimed to specifically investigate the association of the TyG index with the first stroke and first ischemic stroke among elderly individuals with hypertension in the Chinese community.

Methods

Study Design and Participants

Data for our analysis were from the China H-type Hypertension Registry Study (CHRS registration number: ChiCTR1800017274). The CHRS study is an observational, real-world, ongoing prospective cohort study evaluating the treatment, related risk factors and prognosis of patients with hypertension in Wuyuan, Jiangxi Province, China. The study protocol and informed consent were approved by the Ethics Committee of the Anhui Medical University Biomedical Institute. All participants signed the written informed consent form.

The study design and methods of data collection have been described in detail previously.23 Briefly, from March 2018 to August 2018, a total of 14,268 hypertensive patients in the community were enrolled in the CHRS study. At each visit site, face-to-face interviews via a structured questionnaire, anthropometric measurements, and collection of blood samples were completed at baseline. The follow-up investigation was conducted from 31 August 2018 to 31 March 2020, and 14,227 individuals remained in the cohort. We excluded 4324 participants who were younger than 60 years old. After the exclusion of 750 participants with a history of stroke, 459 patients using glucose-lowering drugs, 201 patients using lipoprotein-lowering drugs, and 6 participants without data on the TyG index, a total of 8487 elderly hypertensive patients were selected for the final analysis ().

Data Collection and Definition

The participants underwent the following examinations: a standardized questionnaire, anthropometric measurements, and blood sample collection. The trained medical professionals carried out structured questionnaires, which included age, sex, education, smoking status, alcohol consumption, physical activity, current medication from pill bottles, and previous medical diagnoses. Physical examination items included height, weight, waist circumference (WC), and blood pressure (BP). Body mass index (BMI) was calculated as weight (kg)/height (m2). BP was measured using electronic sphygmomanometer (Omron; Dalian, China) with the subject in a seated position after resting for 10 min, and the average of the three measurements was used. Blood samples of all participants were obtained after an overnight fast of at least 8 hours and stored in the clinical laboratory of the National Clinical Research Center for Kidney Disease, Guangzhou, China. Fasting plasma glucose was assessed by the hexokinase method; serum total cholesterol and serum triglycerides (TGs) were measured by the oxidase method; serum high-density lipoprotein (HDL) cholesterol and serum low-density lipoprotein (LDL) cholesterol were analysed by the direct method; serum uric acid (SUA) was measured by the enzymatic uricase method; serum homocysteine was determined enzymatically; and serum creatinine was analysed by the picric acid method. The blood specimens were measured on automatic clinical analyzer (Beckman Coulter, AU680). The estimated glomerular filtration rate (eGFR) was estimated with the CKD-EPI equation.24 The TyG index was calculated as ln (fasting triglyceride [mg/dL] × fasting glucose [mg/dL]/2).25

Study Outcomes

The outcomes of the study were newly diagnosed stroke, including ischemic stroke. All participants were invited to attend an in-person follow-up visit from 31 August 2018 to 31 March 2020. At each follow-up visit, trained staff used a standard form to query medical history. If patients were hospitalized or had visited an emergency department, trained staff used the forms to record medical information: including medical history, treatments, and diagnosis at discharge from the participants’ medical records. Stroke was recorded from hospital discharge diagnoses (The International Classification of Diseases, ICD-10). The list covers codes included I63 (Ischemic stroke (I63), I60-I61 (Hemorrhagic stroke) and I64 (no type stroke)), confirmed by computed tomography (CT), magnetic resonance imaging (MRI), transcranial, or carotid Doppler sonography. In addition, source data for all suspected stroke cases, including photocopies of the participant’s inpatient record, discharge summary, biochemical tests, and imaging reports were submitted to the Outcome Adjudication Committee for further verification. All members of the committee were unaware of the baseline risk factors for the study participants.

Statistical Analysis

Baseline characteristics are presented as mean values ± standard deviation (SDs) or median (interquartile range) for continuous variables and proportions for categorical variables. Analysis of variance, Kruskal–Wallis test, Fisher’s exact test, or chi-square test were used to compare baseline characteristics by TyG index quartiles, as appropriate. Cox proportional hazards models were used to examine the association of the TyG index with the risk of first stroke and first ischemic stroke in four sequential models. Model 1 was only adjusted for age. Model 2 was additionally adjusted for sex, BMI, WC, education, physical activity, duration of hypertension, current smoking, and current drinking. Model 3 was further adjusted for SBP, DBP, serum homocysteine, SUA, LDL-C, and eGFR. Model 4 was adjusted for model 3 plus diabetes mellitus, atrial fibrillation, coronary heart disease (CHD), antihypertensive drugs, and antiplatelet drugs, which are variables that are known as traditional or suspected risk factors for stroke.26 Adjusted hazard ratios (HRs) and 95% confidence intervals (CIs) were estimated in each regression model. To assess the dose-response relationship of the TyG index with stroke and ischemic stroke, we used a Cox regression model with restricted cubic splines for the TyG index. Effect modification by sex was evaluated via stratified analyses and tested via the interaction term approach. In addition, we also estimated the incidence rates of stroke for each TyG quartile over the total follow-up period. Time-to-event curves were estimated using the Kaplan-Meier method, and the Log rank test was also conducted.

All statistical analyses were conducted with R software (version 3.3.1; ) and Empower (version 2.17.8; ; X&Y Solutions). All P values were 2-sided, and a P value < 0.05 was considered statistically significant.

Results

Basic Characteristics

The baseline characteristics of the subjects according to the TyG index quartiles are shown in Table 1. In the study, a total of 8487 elderly hypertensive patients were evaluated. The mean age was 68.77 years, and 52.76% were men. The mean of baseline TyG index was 8.81. The higher quartiles of the TyG index were more likely to contain patients who were younger; were female; have a higher BMI, WC, SBP, and DBP; have a longer duration of hypertension; were not current smokers or current drinkers; have a history of diabetes mellitus; have a higher level of FPG, TC, TG, LDL-C, and SUA; have a lower level of HDL-C; and were using antihypertensive drugs. (P < 0.001).

Table 1

Baseline Characteristics of the Study Population According to TyG Index Quartiles

	Q1	Q2	Q3	Q4	P-value
N	2122	2121	2122	2122
Age (years)	70.18 ± 6.64	69.24 ± 6.34	68.41 ± 5.88	67.26 ± 5.46	<0.001
Male, n (%)	1427 (67.25)	1043 (49.17)	831 (39.16)	707 (33.32)	<0.001
Education, n (%)					0.356
< high school	1644 (94.65)	1609 (94.31)	1586 (93.29)	1612 (93.78)
≥ high school	93 (5.35)	97 (5.69)	114 (6.71)	107 (6.22)
BMI (kg/m2)	21.22 ± 3.07	22.37 ± 3.30	23.50 ± 3.27	24.45 ± 3.16	<0.001
WC (cm)	77.47 ± 8.79	80.81 ± 9.31	84.32 ± 9.45	86.82 ± 8.70	<0.001
Physical activity, n (%)					0.146
Mild	990 (56.99)	988 (57.91)	1008 (59.29)	1048 (60.97)
Moderate	373 (21.47)	375 (21.98)	347 (20.41)	363 (21.12)
Vigorous	374 (21.53)	343 (20.11)	345 (20.29)	308 (17.92)
Current smoking, n (%)	824 (38.83)	614 (28.95)	497 (23.43)	441 (20.79)	<0.001
Current drinking, n (%)	660 (31.10)	480 (22.63)	412 (19.42)	374 (17.63)	<0.001
SBP (mmHg)	148.23 ± 18.53	149.81 ± 17.75	150.34 ± 17.90	150.78 ± 18.64	<0.001
DBP (mmHg)	85.80 ± 10.79	86.66 ± 10.42	86.88 ± 10.16	87.97 ± 10.22	<0.001
FPG (mmol/L)	5.43 ± 0.60	5.76 ± 0.68	6.00 ± 0.87	6.86 ± 2.05	<0.001
TC (mmol/L)	4.65 ± 0.92	5.08 ± 0.93	5.38 ± 1.04	5.61 ± 1.19	<0.001
TG (mmol/L)	0.79 ± 0.16	1.18 ± 0.17	1.65 ± 0.27	2.95 ± 1.32	<0.001
Serum homocysteine, μmol/L	15.62 (12.99–20.20)	15.94 (13.02–20.46)	15.40 (12.80–19.33)	15.55 (13.01–19.36)	<0.001
HDL-C (mmol/L)	1.76 ± 0.47	1.68 ± 0.42	1.58 ± 0.40	1.44 ± 0.38	<0.001
LDL-C (mmol/L)	2.49 ± 0.63	2.86 ± 0.67	3.17 ± 0.75	3.34 ± 0.84	<0.001
SUA, μmol/L	404.74 ± 113.68	406.57 ± 116.33	419.78 ± 116.50	449.44 ± 126.14	<0.001
eGFR (mL/min per 1.73 m2)	84.03 ± 19.20	83.96 ± 19.08	84.44 ± 18.54	83.20 ± 19.61	0.197
TyG index	8.11 ± 0.22	8.58 ± 0.11	8.95 ± 0.12	9.59 ± 0.37	<0.001
Duration of hypertension, (years)	5.00 (2.00–10.00)	5.00 (2.00–10.00)	6.00 (3.00–10.00)	6.00 (3.00–11.00)	<0.001
Diabetes mellitus, n (%)	38 (1.79)	58 (2.73)	119 (5.61)	278 (13.10)	<0.001
Atrial fibrillation, n (%)	25 (1.44)	27 (1.58)	32 (1.88)	30 (1.75)	0.760
CHD, n (%)	117 (5.51)	108 (5.09)	111 (5.23)	119 (5.61)	0.867
First stroke, n (%)	17 (0.80)	21 (0.99)	16 (0.75)	28 (1.32)	0.223
Antihypertensive drugs, n (%)	1311 (61.78)	1353 (63.79)	1373 (64.73)	1475 (69.54)	<0.001
Antiplatelet drugs, n (%)	35 (1.65)	34 (1.60)	41 (1.93)	38 (1.79)	0.844

Note: Data are the mean± SD, median (interquartile range), or number (percentage).

Abbreviations: BMI, body mass index; WC, waist circumference; SBP, systolic blood pressure; DBP, diastolic blood pressure; FPG, fasting plasma glucose; TC, total cholesterol; TG, triglyceride; HDL-C, high-density lipoprotein cholesterol; LDL-C, low-density lipoprotein cholesterol; eGFR, estimated glomerular filtration rate; TyG, triglyceride glucose; CHD, coronary heart disease.

Association Between the TyG Index and First Stroke

During an average follow-up of 1.72 years, there were 82 incident cases of stroke (0.97%), including 48 patients with ischemic stroke (0.57%). Overall, there was a significant positive association between the baseline TyG index and the risk of first stroke (Figure 1A). Table 2 shows the association of the TyG index with first stroke using multivariate Cox proportional hazard models. In models 1–4, a continuous TyG index was positively correlated with incident stroke. In the fully adjusted model (model 4), each 1-unit increase in the TyG index was associated with a 72% increase in the adjusted risk of first stroke (HR: 1.72; 95% CI: 1.07, 2.76). Consistently, when the TyG index was assessed as quartiles, the adjusted HRs for participants in quartiles 2, 3, and 4 were 1.34 (95% CI: 0.65, 2.74), 1.32 (95% CI: 0.59, 2.95), and 2.40 (95% CI: 1.05, 5.46), respectively, when compared with those in quartile 1 (P-trend < 0.05). A significantly higher risk of first stroke (HR: 1.90; 95% CI: 1.04, 3.45) was found in participants in quartiles 4 compared with participants in quartile 1–3.

Figure 1

Dose–response associations of the TyG index with the risk of first stroke and first ischemic stroke*. (A) TyG index and first stroke; (B) TyG index and first ischemic stroke. *Adjusted for age, sex, BMI, WC, education, physical activity, duration of hypertension, current smoking, current drinking, BP, DBP, serum homocysteine, SUA, LDL-C, eGFR, diabetes mellitus, atrial fibrillation, CHD, anti-hypertensive drugs, anti-platelet drugs.

Table 2

The Association Between Baseline TyG Index and Incident Risk of First Stroke

TyG Index	Events (%)	First Stroke, HR (95% CI)
		Model 1	Model 2	Model 3	Model 4
Per 1 unit increase	82/8487 (0.97)	1.53 (1.07, 2.20)	1.82 (1.19, 2.80)	1.72 (1.07, 2.75)	1.72 (1.07, 2.76)
Quartiles
Q 1 (7.15–8.38)	17/2122 (0.80)	1	1	1	1
Q 2 (8.39–8.75)	21/2121 (0.99)	1.32 (0.69, 2.50)	1.45 (0.72, 2.95)	1.30 (0.63, 2.65)	1.34 (0.65, 2.74)
Q 3 (8.76–9.15)	16/2122 (0.75)	1.07 (0.54, 2.12)	1.42 (0.66, 3.09)	1.31 (0.58, 2.93)	1.32 (0.59, 2.95)
Q 4 (9.16–11.73)	28/2122 (1.32)	2.04 (1.11, 3.78)	2.67 (1.26, 5.64)	2.34 (1.04, 5.28)	2.40 (1.05, 5.46)
P-trend		0.041	0.014	0.048	0.048
Categories
Q1-Q3 (7.15–9.15)	54/6365 (0.85)	1	1	1	1
Q4 (> 9.15)	28/2122 (1.32)	1.81 (1.14, 2.88)	2.04 (1.17, 3.56)	1.88 (1.04, 3.40)	1.90 (1.04, 3.45)

Notes: Data are regression coefficients (95% confidence interval). A test for linear trend was conducted with the use of quartiles of the TyG index as a continuous variable by assigning the median values of the quartiles to the variable. Model 1 is adjusted for age. Model 2 is further adjusted for sex, BMI, WC, education, physical activity, duration of hypertension, current smoking, current drinking. Model 3 is further adjusted for SBP, DBP, serum homocysteine, SUA, LDL-C, eGFR. Model 4 is further adjusted for diabetes mellitus, atrial fibrillation, CHD, anti-hypertensive drugs, anti-platelet drugs.

Abbreviations: TyG, triglyceride glucose; HR, hazard ratio; CI, confidence interval.

The association between the TyG index and the first ischemic stroke is presented in Figure 1B. Similarly, each 1-unit increase in the TyG index was associated with a 2.31-fold increase in the adjusted risk of first ischemic stroke (HR: 2.31; 95% CI: 1.32, 4.05). Accordingly, participants in quartile 4 had a higher risk of first ischemic stroke (HR: 2.45; 95% CI: 1.16, 5.20) compared with participants in quartile 1–3 (Table 3).

Table 3

The Association Between Baseline TyG Index and Incident Risk of First Ischemic Stroke

TyG Index	Events (%)	First Ischemic Stroke, HR (95% CI)
		Model 1	Model 2	Model 3	Model 4
Per 1 unit increase		2.23 (1.43, 3.48)	2.47 (1.47, 4.13)	2.31 (1.32, 4.05)	2.31 (1.32, 4.05)
Quartiles	48/8487 (0.57)
Q 1 (7.15–8.38)	6/2122 (0.28)	1	1	1	1
Q 2 (8.39–8.75)	13/2121 (0.61)	2.32 (0.88, 6.10)	2.33 (0.85, 6.42)	2.09 (0.75, 5.79)	2.14 (0.77, 5.95)
Q 3 (8.76–9.15)	9/2122 (0.42)	1.71 (0.60, 4.82)	1.72 (0.55, 5.38)	1.49 (0.46, 4.85)	1.52 (0.47, 4.94)
Q 4 (9.16–11.73)	20/2122 (0.94)	4.15 (1.65, 10.49)	4.63 (1.62, 13.26)	3.92 (1.26, 12.16)	3.99 (1.28, 12.48)
P-trend		0.004	0.007	0.028	0.028
Categories
Q1-Q3 (7.15–9.15)	28/6365 (0.44)	1	1	1	1
Q4 (> 9.15)	20/2122 (0.94)	2.49 (1.39, 4.47)	2.71 (1.34, 5.46)	2.46 (1.17, 5.18)	2.45 (1.16, 5.20)

Notes: Data are regression coefficients (95% confidence interval). A test for linear trend was conducted with the use of quartiles of the TyG index as a continuous variable by assigning the median values of the quartiles to the variable. Model 1 is adjusted for age. Model 2 is further adjusted for sex, BMI, WC, education, physical activity, duration of hypertension, current smoking, current drinking. Model 3 is further adjusted for SBP, DBP, serum homocysteine, SUA, LDL-C, eGFR. Model 4 is further adjusted for diabetes mellitus, atrial fibrillation, CHD, anti-hypertensive drugs, anti-platelet drugs.

Abbreviations: TyG, triglyceride glucose; HR, hazard ratio; CI, confidence interval.

The Kaplan–Meier survival curve for first stroke and ischemic stroke stratified by the TyG index is shown in Figure 2. Compared with the quartile 1–3 of the TyG index, patients in quartile 4 had a higher risk of first stroke and ischemic stroke (log-rank P < 0.05).

Figure 2

Kaplan–Meier curves of incidence of first stroke and first ischemic stroke according to the baseline TyG index. (A) TyG index and first stroke; (B) TyG index and first ischemic stroke.

Sensitivity Analysis

We further performed Cox regression analyses to assess the association of the TyG index and stroke after excluding patients with a history of diabetes or diabetes with obesity. Quartile 4 of the baseline TyG index was similarly associated with an increased risk of first stroke and first ischemic stroke in nondiabetic patients and nondiabetic obese patients (). In addition, the association of TyG index with the first stroke and ischemic stroke were consistent in the different genders (P-interaction = 0.289 and 0.768, and ).

Discussion

In this large-scale, prospective, hypertensive population-based cohort study, we found that higher levels of the TyG index at baseline were associated with an increased risk of first stroke and first ischemic stroke in elderly hypertensive patients. This finding persisted even after adjusting for as many cardiovascular risk factors as possible. This study contributes new information concerning older adults with hypertension, a group in whom IR is common and stroke risk is high, and for whom data are relatively lacking from prior studies.

Recently, several studies have shown an association between the TyG index and stroke. In a cross-sectional study of 10,900 subjects from China,20 Shi et al found the TyG index was positively associated with ischemic stroke. Data from the Kailuan Study,19 a study of 97,653 participants free of a history of stroke during a median of 11.02 years of follow-up by Wang et al, found that both the baseline and long-term updated cumulative average TyG index can predict stroke and ischemic stroke but not intracerebral haemorrhage. Zhao et al22 and Hong et al17 consistently reported that a high TyG index was significantly associated with an increased risk of stroke. In addition, a Mendelian randomization study by Si et al18 of 273,368 individuals of European ancestry showed that the TyG index was significantly associated with several cerebrovascular diseases. Collectively, these findings suggested that the TyG index, a simple measure reflecting insulin resistance, was strongly correlated with the risk of stroke. However, apart from a few patient cohort studies,27–29 most of the evidence is derived from general populations, rather than from stroke event-driven studies in high-risk individuals. Given older adults with hypertension represent a specific high-risk group for accelerated incident stroke,30 whether the TyG index predicts the first stroke outcome remains uncertain. Therefore, we conducted our study to investigate the association. Consistent with previous studies, the results in the main analysis of the study support that a higher TyG index was significantly associated with an increased risk of future stroke in elderly patients with hypertension.

The exact mechanism underlying the relationship between the TyG index and stroke has not been fully elucidated. The TyG index has been proposed as a reliable marker of IR.25,31 Thus, IR may mainly account for these associations. First, IR plays an important role in endothelial dysfunction,32 chronic inflammation,33 and platelet adhesion, activation, and aggregation.34,35 Second, IR was associated with the development of atherosclerosis, plaque progression, and rupture.29,36,37 Third, IR may be a mechanism that leads to hypertension, diabetes,11 dyslipidaemia, and other factors,38 ultimately increasing stroke risk.14 Additionally, the TyG index, which is calculated from fasting glucose and triglycerides, was positively associated with hyperglycaemia and dyslipidaemia, suggesting that the TyG index may influence stroke risk through the presence of cardiovascular risk factors.

There are some limitations in this study. First, glycosylated haemoglobin and 2-h oral glucose tolerance tests were not examined, so the impact of impaired glucose homeostasis on stroke could not be assessed. Second, although the most available demographic and clinical variables were included in the regression models, residual confounding factors could have affected the results. Third, due to the sample size being too small for haemorrhagic stroke, we did not analyse this type of stroke separately. Fourth, the TyG index was only assessed at baseline, and more frequent measurements would possibly have provided more information. Finally, the study was limited to a specific subpopulation, so our findings cannot be extrapolated to other populations.

In summary, using data from a prospective, large-scale hypertension registry study, we found that a high TyG index representing IR was independently associated with an increased risk of first stroke. These findings suggested that the TyG index may be a useful marker for risk stratification in elderly patients with hypertension.