| Literature DB >> 35152390 |
Bei Gao1,2, Mo-Xian Chen1,3, Fu-Yuan Zhu4.
Abstract
SWATH-MS proteomic approaches enable the identification and quantification of thousands of proteins within a single profiling experiment, which is useful for the identification of genes regulated by abscisic acid (ABA) in a high-throughput manner. Here we describe the experimental procedures for protein extraction, digestion, peptides desalting, followed by the establishment of a DDA spectrum database and DIA-based SWATH detection and protein quantification. This method is able to identify and quantify proteins involved in ABA metabolism, signal perception and transduction with high accuracy and reproducibility.Entities:
Keywords: Abscisic acid; Arabidopsis thaliana; DDA; DIA; Label-free; Proteome; Rice
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Year: 2022 PMID： 35152390 DOI： 10.1007/978-1-0716-2156-1_15
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745