S Canosa1, D Cimadomo2, A Conforti3, R Maggiulli4, A Giancani2, A Tallarita5, F Golia6, G Fabozzi2, A Vaiarelli2, G Gennarelli1, A Revelli1, F Bongioanni1, C Alviggi3, F M Ubaldi2, L Rienzi2. 1. Livet, GeneraLife IVF, Turin, Italy. 2. Clinica Valle Giulia, GeneraLife IVF, Rome, Italy. 3. Department of Neuroscience, Reproductive Science and Odontostomatology, University of Naples Federico II University, Naples, Italy. 4. Clinica Valle Giulia, GeneraLife IVF, Rome, Italy. maggiulli@generaroma.it. 5. Genera Veneto, GeneraLife IVF, Marostica, Italy. 6. Clinica Ruesch, GeneraLife IVF, Naples, Italy.
Abstract
PURPOSE: Few studies explored whether prolonged cryo-storage after vitrification affects embryo competence and perinatal outcomes. This systematic review and meta-analysis aims at highlighting any putative impact of cryo-storage duration on cryo-survival, miscarriage, live birth and major malformations. METHODS: A systematic review was performed using MEDLINE (PubMed), ISI Web of Knowledge, Scopus and Embase databases up to June 2021. Data were combined to obtain a pooled OR, and meta-analysis was conducted using a random effects model. Out of 1,389 screened abstracts, 22 papers were assessed for eligibility, and 5 studies were included (N = 18,047 embryos). Prolonged cryo-storage was defined as > 12 months (N = 3389 embryos). Subgroup analysis was performed for untested vitrified cleavage stage embryos (N = 1739 embryos) and for untested and euploid vitrified blastocysts (N = 13,596 and 2712 embryos, respectively). RESULTS: Survival rate, miscarriage, live birth and major malformation rates were all similar in the two groups. CONCLUSION: These data further support the safety of long-term cryo-storage of human embryos beyond 12 months. This is reassuring for good prognosis patients with surplus embryos, couples seeking a second child from supernumerary embryos and women postponing the transfer for clinical or personal reasons.
PURPOSE: Few studies explored whether prolonged cryo-storage after vitrification affects embryo competence and perinatal outcomes. This systematic review and meta-analysis aims at highlighting any putative impact of cryo-storage duration on cryo-survival, miscarriage, live birth and major malformations. METHODS: A systematic review was performed using MEDLINE (PubMed), ISI Web of Knowledge, Scopus and Embase databases up to June 2021. Data were combined to obtain a pooled OR, and meta-analysis was conducted using a random effects model. Out of 1,389 screened abstracts, 22 papers were assessed for eligibility, and 5 studies were included (N = 18,047 embryos). Prolonged cryo-storage was defined as > 12 months (N = 3389 embryos). Subgroup analysis was performed for untested vitrified cleavage stage embryos (N = 1739 embryos) and for untested and euploid vitrified blastocysts (N = 13,596 and 2712 embryos, respectively). RESULTS: Survival rate, miscarriage, live birth and major malformation rates were all similar in the two groups. CONCLUSION: These data further support the safety of long-term cryo-storage of human embryos beyond 12 months. This is reassuring for good prognosis patients with surplus embryos, couples seeking a second child from supernumerary embryos and women postponing the transfer for clinical or personal reasons.
Authors: Ana Cobo; María José de los Santos; Damià Castellò; Pilar Gámiz; Pilar Campos; José Remohí Journal: Fertil Steril Date: 2012-08-03 Impact factor: 7.329
Authors: M Simopoulou; K Sfakianoudis; P Giannelou; A Rapani; E Maziotis; P Tsioulou; S Grigoriadis; E Simopoulos; D Mantas; M Lambropoulou; M Koutsilieris; K Pantos; J C Harper Journal: J Assist Reprod Genet Date: 2019-12-01 Impact factor: 3.412
Authors: Laura Rienzi; Clarisa Gracia; Roberta Maggiulli; Andrew R LaBarbera; Daniel J Kaser; Filippo M Ubaldi; Sheryl Vanderpoel; Catherine Racowsky Journal: Hum Reprod Update Date: 2017-03-01 Impact factor: 15.610