OBJECTIVE: To report a birth of a healthy girl after long-term oocyte cryopreservation by slow cooling in sodium depleted medium. DESIGN: Clinical application. SETTING: University Affiliated, Private IVF center. PATIENT: A 38-year-old woman received embryos from IVF by intracytoplasmic sperm injection (ICSI) with her own oocytes that were cryopreserved by slow freezing in a low-sodium medium 14 years and 6 months before, when she was 24 years old. MAIN OUTCOME MEASURE(S): Survival, fertilization, cleavage, clinical pregnancy and delivery. RESULT(S): From six metaphase-II oocytes thawed, two survived, one was fertilized after ICSI and a cleaving embryo was transferred on day 3. A single term pregnancy was achieved, ending with the delivery of a healthy girl. CONCLUSION(S): Cryopreservation after slow freezing in a sodium depleted medium maintained the developmental competence of oocytes after long-term storage and resulted in a successful live birth. As far as is known, this case represents, up to date, the longest storage period of cryopreserved human oocytes resulting in a live birth.
OBJECTIVE: To report a birth of a healthy girl after long-term oocyte cryopreservation by slow cooling in sodium depleted medium. DESIGN: Clinical application. SETTING: University Affiliated, Private IVF center. PATIENT: A 38-year-old woman received embryos from IVF by intracytoplasmic sperm injection (ICSI) with her own oocytes that were cryopreserved by slow freezing in a low-sodium medium 14 years and 6 months before, when she was 24 years old. MAIN OUTCOME MEASURE(S): Survival, fertilization, cleavage, clinical pregnancy and delivery. RESULT(S): From six metaphase-II oocytes thawed, two survived, one was fertilized after ICSI and a cleaving embryo was transferred on day 3. A single term pregnancy was achieved, ending with the delivery of a healthy girl. CONCLUSION(S): Cryopreservation after slow freezing in a sodium depleted medium maintained the developmental competence of oocytes after long-term storage and resulted in a successful live birth. As far as is known, this case represents, up to date, the longest storage period of cryopreserved human oocytes resulting in a live birth.
Authors: L Parmegiani; C Garello; F Granella; D Guidetti; S Bernardi; G E Cognigni; A Revelli; M Filicori Journal: Reprod Biomed Online Date: 2009-09 Impact factor: 3.828
Authors: Dunsong Yang; Samuel E Brown; Kevin Nguyen; Vijay Reddy; Cindy Brubaker; Kevin L Winslow Journal: Fertil Steril Date: 2007-04-06 Impact factor: 7.329
Authors: S Canosa; D Cimadomo; A Conforti; R Maggiulli; A Giancani; A Tallarita; F Golia; G Fabozzi; A Vaiarelli; G Gennarelli; A Revelli; F Bongioanni; C Alviggi; F M Ubaldi; L Rienzi Journal: J Assist Reprod Genet Date: 2022-02-04 Impact factor: 3.357
Authors: Yolanda Segovia; Noemí Victory; Irene Peinado; Laura M García-Valverde; Magdalena García; Jon Aizpurua; Ana Monzó; María José Gómez-Torres Journal: J Reprod Dev Date: 2017-04-30 Impact factor: 2.214