Literature DB >> 34469558

Triplex Direct Quantitative Polymerase Chain Reaction for the Identification of Streptococcus pneumoniae Serotypes.

Mahamoudou Ouattara1, Mamadou Tamboura2, Dinanibè Kambiré2, Kim Anh Lê3, Thanh Van Phan4, Srinivasan Velusamy1, Hien Anh Nguyen3, Dai Vo Thi Trang4, Fernanda C Lessa1, Makiko Iijima5, Dac Trung Nguyen3, Stephanie B Schwartz1, Lesley McGee1, Rasmata Ouédraogo Traoré2, Bernard Beall1.   

Abstract

The quantitative polymerase chain reaction (qPCR) method presented in this study allows the identification of pneumococcal capsular serotypes in cerebrospinal fluid without first performing DNA extraction. This testing approach, which saves time and resources, demonstrated similar sensitivity and a high level of agreement between cycle threshold values when it was compared side-by-side with the standard qPCR method with extracted DNA. Published by Oxford University Press for the Infectious Diseases Society of America 2021.

Entities:  

Keywords:  zzm321990 Streptococcus pneumoniaezzm321990 ; direct real-time PCR; pneumococcal serotype

Mesh：

Year:  2021        PMID： 34469558      PMCID： PMC8414907          DOI： 10.1093/infdis/jiab056

Source DB:  PubMed          Journal:  J Infect Dis        ISSN： 0022-1899            Impact factor:   7.759


  10 in total

1.  Why Bland-Altman plots should use X, not (Y+X)/2 when X is a reference method.

Authors:  Jan S Krouwer
Journal:  Stat Med       Date:  2008-02-28       Impact factor: 2.373

2.  Triplex real-time PCR assay for the detection of Streptococcus pneumoniae, Neisseria meningitidis and Haemophilus influenzae directly from clinical specimens without extraction of DNA.

Authors:  Mahamoudou Ouattara; Melissa J Whaley; Laurel T Jenkins; Stephanie B Schwartz; Rasmata Ouédraogo Traoré; Seydou Diarra; Jean-Marc Collard; Claudio T Sacchi; Xin Wang
Journal:  Diagn Microbiol Infect Dis       Date:  2018-10-16       Impact factor: 2.803

3.  Sequential triplex real-time PCR assay for detecting 21 pneumococcal capsular serotypes that account for a high global disease burden.

Authors:  Fabiana C Pimenta; Alexis Roundtree; Ahmet Soysal; Mustafa Bakir; Mignon du Plessis; Nicole Wolter; Anne von Gottberg; Lesley McGee; Maria da Gloria Carvalho; Bernard Beall
Journal:  J Clin Microbiol       Date:  2012-12-05       Impact factor: 5.948

4.  Expanded sequential quadriplex real-time polymerase chain reaction (PCR) for identifying pneumococcal serotypes, penicillin susceptibility, and resistance markers.

Authors:  Srinivasan Velusamy; Theresa Tran; Thitipong Mongkolrattanothai; Hollis Walker; Lesley McGee; Bernard Beall
Journal:  Diagn Microbiol Infect Dis       Date:  2020-03-12       Impact factor: 2.803

5.  Statistical methods for assessing agreement between two methods of clinical measurement.

Authors:  J M Bland; D G Altman
Journal:  Lancet       Date:  1986-02-08       Impact factor: 79.321

Review 6.  Streptococcus pneumoniae: transmission, colonization and invasion.

Authors:  Jeffrey N Weiser; Daniela M Ferreira; James C Paton
Journal:  Nat Rev Microbiol       Date:  2018-06       Impact factor: 60.633

7.  Identification of pneumococcal serotypes from culture-negative clinical specimens by novel real-time PCR.

Authors:  D Tarragó; A Fenoll; D Sánchez-Tatay; L A Arroyo; C Muñoz-Almagro; C Esteva; W P Hausdorff; J Casal; I Obando
Journal:  Clin Microbiol Infect       Date:  2008-09       Impact factor: 8.067

8.  Evaluation and improvement of real-time PCR assays targeting lytA, ply, and psaA genes for detection of pneumococcal DNA.

Authors:  Maria da Gloria S Carvalho; Maria Lucia Tondella; Karen McCaustland; Luciana Weidlich; Lesley McGee; Leonard W Mayer; Arnold Steigerwalt; Melissa Whaley; Richard R Facklam; Barry Fields; George Carlone; Edwin W Ades; Ron Dagan; Jacquelyn S Sampson
Journal:  J Clin Microbiol       Date:  2007-05-30       Impact factor: 5.948

9.  Single-plex quantitative assays for the detection and quantification of most pneumococcal serotypes.

Authors:  Fuminori Sakai; Sopio Chochua; Catherine Satzke; Eileen M Dunne; Kim Mulholland; Keith P Klugman; Jorge E Vidal
Journal:  PLoS One       Date:  2015-03-23       Impact factor: 3.240

10.  A New Pneumococcal Capsule Type, 10D, is the 100th Serotype and Has a Large cps Fragment from an Oral Streptococcus.

Authors:  Feroze Ganaie; Jamil S Saad; Lesley McGee; Andries J van Tonder; Stephen D Bentley; Stephanie W Lo; Rebecca A Gladstone; Paul Turner; Jeremy D Keenan; Robert F Breiman; Moon H Nahm
Journal:  mBio       Date:  2020-05-19       Impact factor: 7.867
  10 in total

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