Literature DB >> 30413354

Triplex real-time PCR assay for the detection of Streptococcus pneumoniae, Neisseria meningitidis and Haemophilus influenzae directly from clinical specimens without extraction of DNA.

Mahamoudou Ouattara1, Melissa J Whaley2, Laurel T Jenkins2, Stephanie B Schwartz2, Rasmata Ouédraogo Traoré3, Seydou Diarra4, Jean-Marc Collard5, Claudio T Sacchi6, Xin Wang2.   

Abstract

This study presents a triplex real-time PCR assay that allows for the direct detection of Streptococcus pneumoniae, Neisseria meningitidis, and Haemophilus influenzae in one reaction without DNA extraction, with similar sensitivity and specificity to singleplex assays. This approach saves time, specimen volume and reagents while achieving a higher testing throughput. Published by Elsevier Inc.

Entities:  

Keywords:  Bacteria; Cerebrospinal; Detection; Meningitis; Real-time PCR; Triplex

Mesh：

Year:  2018        PMID： 30413354     DOI： 10.1016/j.diagmicrobio.2018.10.008

Source DB:  PubMed          Journal:  Diagn Microbiol Infect Dis        ISSN： 0732-8893            Impact factor:   2.803


  7 in total

1.  Triplex Direct Quantitative Polymerase Chain Reaction for the Identification of Streptococcus pneumoniae Serotypes.

Authors:  Mahamoudou Ouattara; Mamadou Tamboura; Dinanibè Kambiré; Kim Anh Lê; Thanh Van Phan; Srinivasan Velusamy; Hien Anh Nguyen; Dai Vo Thi Trang; Fernanda C Lessa; Makiko Iijima; Dac Trung Nguyen; Stephanie B Schwartz; Lesley McGee; Rasmata Ouédraogo Traoré; Bernard Beall
Journal:  J Infect Dis       Date:  2021-09-01       Impact factor: 7.759

2.  Direct Real-Time PCR for the Detection and Serotyping of Haemophilus influenzae without DNA Extraction.

Authors:  Daya Marasini; Melissa J Whaley; Laurel T Jenkins; Fang Hu; Wenxin Jiang; Nadav Topaz; Alex Chen; Susanna Schmink; Jennifer Dolan Thomas; Brian H Harcourt; Henju Marjuki; Xin Wang
Journal:  J Clin Microbiol       Date:  2022-03-21       Impact factor: 11.677

3.  Multiplex real-time PCR using SYBR Green: Unspecific intercalating dye to detect antimicrobial resistance genes of Streptococcus pneumoniae in cerebrospinal fluid.

Authors:  Mariana Brena Souza; Maria Cecília Cergole-Novella; Delma Aparecida Molinari; Daniela Rodrigues Colpas; Andréia Moreira Dos Santos Carmo; Vilma Dos Santos Menezes Gaiotto Daros; Ivana Barros de Campos
Journal:  PLoS One       Date:  2022-06-14       Impact factor: 3.752

4.  Etiology of meningitis among adults in three quaternary hospitals in Mozambique, 2016-2017: The role of HIV.

Authors:  Aquino Albino Nhantumbo; Charlotte Elizabeth Comé; Plácida Iliany Maholela; Alcides Moniz Munguambe; Paulino da Costa; Mariana Mott; Gabriella Rosa Cunha; Lúcia Chambal; Cícero Dias; Vlademir Vicente Cantarelli; Eduardo Samo Gudo
Journal:  PLoS One       Date:  2022-05-11       Impact factor: 3.752

5.  Comparison of Culture, Antigen Test, and Polymerase Chain Reaction for Pneumococcal Detection in Cerebrospinal Fluid of Children.

Authors:  Md Hasanuzzaman; Senjuti Saha; Roly Malaker; Hafizur Rahman; Mohammad S I Sajib; Rajib C Das; Maksuda Islam; Davidson H Hamer; Gary L Darmstadt; Samir K Saha
Journal:  J Infect Dis       Date:  2021-09-01       Impact factor: 5.226

6.  The Global Landscape of Pediatric Bacterial Meningitis Data Reported to the World Health Organization-Coordinated Invasive Bacterial Vaccine-Preventable Disease Surveillance Network, 2014-2019.

Authors:  Tomoka Nakamura; Adam L Cohen; Stephanie Schwartz; Jason M Mwenda; Goitom Weldegebriel; Joseph N M Biey; Reggis Katsande; Amany Ghoniem; Kamal Fahmy; Hossam Abdel Rahman; Dovile Videbaek; Danni Daniels; Simarjit Singh; Annemarie Wasley; Gloria Rey-Benito; Lucia de Oliveira; Claudia Ortiz; Emmanuel Tondo; Jayantha B L Liyanage; Mohammad Sharifuzzaman; Varja Grabovac; Nyambat Batmunkh; Josephine Logronio; James Heffelfinger; Kimberly Fox; Linda De Gouveia; Anne von Gottberg; Mignon Du Plessis; Brenda Kwambana-Adams; Martin Antonio; Samaa El Gohary; Aya Azmy; Asmaa Gamal; Elena Voropaeva; Ekaterina Egorova; Yulia Urban; Carolina Duarte; Balaji Veeraraghavan; Samir Saha; Ben Howden; Michelle Sait; Sangoun Jung; Songmee Bae; David Litt; Shila Seaton; Mary Slack; Sebastien Antoni; Mahamoudou Ouattara; Chris Van Beneden; Fatima Serhan
Journal:  J Infect Dis       Date:  2021-09-01       Impact factor: 5.226

7.  Identification of Streptococcus suis Meningitis by Direct Triplex Real-Time PCR, Burkina Faso.

Authors:  Mahamoudou Ouattara; Mamadou Tamboura; Dinanibe Kambire; Mahamoudou Sanou; Kalifa Ouattara; Malika Congo; Adama Kaboré; Soufiane Sanou; Elie Kabré; Sable Sharpley; Theresa Tran; Stephanie Schwartz; Soumeya Ouangraoua; Abdoul-Salam Ouedraogo; Lassana Sangaré; Rasmata Ouedraogo-Traore; Cynthia G Whitney; Bernard Beall
Journal:  Emerg Infect Dis       Date:  2020-09       Impact factor: 6.883
  7 in total

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