Literature DB >> 23224094

Sequential triplex real-time PCR assay for detecting 21 pneumococcal capsular serotypes that account for a high global disease burden.

Fabiana C Pimenta1, Alexis Roundtree, Ahmet Soysal, Mustafa Bakir, Mignon du Plessis, Nicole Wolter, Anne von Gottberg, Lesley McGee, Maria da Gloria Carvalho, Bernard Beall.   

Abstract

We developed and validated a real-time PCR assay consisting of 7 triplexed reactions to identify 11 individual serotypes plus 10 small serogroups representing the majority of disease-causing isolates of Streptococcus pneumoniae. This assay targets the 13 serotypes included within the 13-valent conjugate vaccine and 8 additional key serotypes or serogroups. Advantages over other serotyping assays are described. The assay will be expanded to 40 serotypes/serogroups. We will provide periodic updates at our protocol website.

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Year:  2012        PMID： 23224094      PMCID： PMC3553924          DOI： 10.1128/JCM.02927-12

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  22 in total

1.  Potential nonpneumococcal confounding of PCR-based determination of serotype in carriage.

Authors:  Maria da Gloria Carvalho; Godfrey M Bigogo; Muthoni Junghae; Fabiana C Pimenta; Iaci Moura; Alexis Roundtree; Zhongya Li; Laura Conklin; Daniel R Feikin; Robert F Breiman; Cynthia G Whitney; Bernard Beall
Journal:  J Clin Microbiol       Date:  2012-07-03       Impact factor: 5.948

2.  Multiplex PCR-based reverse line blot hybridization assay to identify 23 Streptococcus pneumoniae polysaccharide vaccine serotypes.

Authors:  Fanrong Kong; Mitchell Brown; Archcna Sabananthan; Xianyu Zeng; Gwendolyn L Gilbert
Journal:  J Clin Microbiol       Date:  2006-05       Impact factor: 5.948

3.  Identification of less-common Streptococcus pneumoniae serotypes by a multiplex PCR-based reverse line blot hybridization assay.

Authors:  Fei Zhou; Fanrong Kong; Zhongsheng Tong; Gwendolyn L Gilbert
Journal:  J Clin Microbiol       Date:  2007-08-08       Impact factor: 5.948

4.  Serotyping pneumococcal meningitis cases in the African meningitis belt by use of multiplex PCR with cerebrospinal fluid.

Authors:  Berthe-Marie Njanpop Lafourcade; Oumarou Sanou; Mark van der Linden; Natalia Levina; Meryem Karanfil; Seydou Yaro; Tsidi A Tamekloe; Judith E Mueller
Journal:  J Clin Microbiol       Date:  2009-12-09       Impact factor: 5.948

5.  Simple, accurate, serotype-specific PCR assay to differentiate Streptococcus pneumoniae serotypes 6A, 6B, and 6C.

Authors:  Ping Jin; Meng Xiao; Fanrong Kong; Shahin Oftadeh; Fei Zhou; Chunyi Liu; Gwendolyn L Gilbert
Journal:  J Clin Microbiol       Date:  2009-06-17       Impact factor: 5.948

6.  Differential effects of pneumococcal vaccines against serotypes 6A and 6C.

Authors:  In H Park; Matthew R Moore; John J Treanor; Stephen I Pelton; Tamara Pilishvili; Bernard Beall; Mark A Shelly; Barbara E Mahon; Moon H Nahm
Journal:  J Infect Dis       Date:  2008-12-15       Impact factor: 5.226

7.  Molecular detection methods and serotyping performed directly on clinical samples improve diagnostic sensitivity and reveal increased incidence of invasive disease by Streptococcus pneumoniae in Italian children.

Authors:  Chiara Azzari; Maria Moriondo; Giuseppe Indolfi; Cristina Massai; Laura Becciolini; Maurizio de Martino; Massimo Resti
Journal:  J Med Microbiol       Date:  2008-10       Impact factor: 2.472

8.  Identification of pneumococcal serotypes from culture-negative clinical specimens by novel real-time PCR.

Authors:  D Tarragó; A Fenoll; D Sánchez-Tatay; L A Arroyo; C Muñoz-Almagro; C Esteva; W P Hausdorff; J Casal; I Obando
Journal:  Clin Microbiol Infect       Date:  2008-09       Impact factor: 8.067

9.  PCR-based quantitation and clonal diversity of the current prevalent invasive serogroup 6 pneumococcal serotype, 6C, in the United States in 1999 and 2006 to 2007.

Authors:  Maria da Gloria Carvalho; Fabiana C Pimenta; Robert E Gertz; Hari Har Joshi; Alma A Trujillo; Logan E Keys; Joy Findley; Iaci S Moura; In H Park; Susan K Hollingshead; Tamara Pilishvili; Cynthia G Whitney; Moon H Nahm; Bernard W Beall
Journal:  J Clin Microbiol       Date:  2008-12-30       Impact factor: 5.948

10.  Identification of serotype in culture negative pneumococcal meningitis using sequential multiplex PCR: implication for surveillance and vaccine design.

Authors:  Samir K Saha; Gary L Darmstadt; Abdullah H Baqui; Belal Hossain; Maksuda Islam; Dona Foster; Hassan Al-Emran; Aliya Naheed; Shams El Arifeen; Stephen P Luby; Mathuram Santosham; Derrick Crook
Journal:  PLoS One       Date:  2008-10-31       Impact factor: 3.240
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  65 in total

1.  Challenges of using molecular serotyping for surveillance of pneumococcal disease.

Authors:  Victoria Magomani; Nicole Wolter; Stefano Tempia; Mignon du Plessis; Linda de Gouveia; Anne von Gottberg
Journal:  J Clin Microbiol       Date:  2014-06-23       Impact factor: 5.948

2.  Development for Clinical Use of a Multiplexed Immunoassay Using Sputum Samples for Streptococcus pneumoniae: a Non-Culture-Based Approach for Serotype-Specific Detection.

Authors:  Sun Jin Kim; Yoo Jung Jeong; Jong Hun Kim; Young Kyung Yoon; Jang Wook Sohn; Moon Hee Nahm; Min Ja Kim
Journal:  J Clin Microbiol       Date:  2019-09-24       Impact factor: 5.948

3.  Direct detection and prediction of all pneumococcal serogroups by target enrichment-based next-generation sequencing.

Authors:  Margaret Ip; Veranja Liyanapathirana; Irene Ang; Kitty S C Fung; T K Ng; Haokui Zhou; Dominic N C Tsang
Journal:  J Clin Microbiol       Date:  2014-10-01       Impact factor: 5.948

4.  Single-Step Multiplex PCR Assay for Determining 92 Pneumococcal Serotypes.

Authors:  José M Marimón; María Ercibengoa; Erica Santacatterina; Marta Alonso; Emilio Pérez-Trallero
Journal:  J Clin Microbiol       Date:  2016-06-08       Impact factor: 5.948

5.  Distribution of serotypes and antibiotic susceptibility patterns among invasive pneumococcal diseases in Saudi Arabia.

Authors:  Yazeed A Al-Sheikh; Yazeed A Al-Sherikh; Lakshmana K Gowda; M Marie Mohammed Ali; James John; Dabwan Khaled Homoud Mohammed; Pradeep Chikkabidare Shashidhar
Journal:  Ann Lab Med       Date:  2014-04-08       Impact factor: 3.464

6.  Competitive Dominance within Biofilm Consortia Regulates the Relative Distribution of Pneumococcal Nasopharyngeal Density.

Authors:  Xueqing Wu; Nathan T Jacobs; Catherine Bozio; Preston Palm; Santiago M Lattar; Christiane R Hanke; David M Watson; Fuminori Sakai; Bruce R Levin; Keith P Klugman; Jorge E Vidal
Journal:  Appl Environ Microbiol       Date:  2017-08-01       Impact factor: 4.792

Review 7.  Pneumococcal Capsules and Their Types: Past, Present, and Future.

Authors:  K Aaron Geno; Gwendolyn L Gilbert; Joon Young Song; Ian C Skovsted; Keith P Klugman; Christopher Jones; Helle B Konradsen; Moon H Nahm
Journal:  Clin Microbiol Rev       Date:  2015-07       Impact factor: 26.132

8.  Development of an Extended-Specificity Multiplex Immunoassay for Detection of Streptococcus pneumoniae Serotype-Specific Antigen in Urine by Use of Human Monoclonal Antibodies.

Authors:  Seyi D Eletu; Carmen L Sheppard; Elizabeth Thomas; Kenneth Smith; Priya Daniel; David J Litt; Wei Shen Lim; Norman K Fry
Journal:  Clin Vaccine Immunol       Date:  2017-12-05

9.  Effectiveness of ten-valent pneumococcal conjugate vaccine against invasive pneumococcal disease in Brazil: a matched case-control study.

Authors:  Carla Magda Allan S Domingues; Jennifer R Verani; Ernesto Issac Montenegro Renoiner; Maria Cristina de Cunto Brandileone; Brendan Flannery; Lucia Helena de Oliveira; João Barberino Santos; José Cássio de Moraes
Journal:  Lancet Respir Med       Date:  2014-04-10       Impact factor: 30.700

10.  Impact of 13-Valent Pneumococcal Conjugate Vaccine on Pneumococcal Meningitis, Burkina Faso, 2016-2017.

Authors:  Heidi M Soeters; Dinanibè Kambiré; Guetawendé Sawadogo; Rasmata Ouédraogo-Traoré; Brice Bicaba; Isaïe Medah; Lassana Sangaré; Abdoul-Salam Ouédraogo; Soumeya Ouangraoua; Issaka Yaméogo; Malika Congo-Ouédraogo; Absatou Ky Ba; Flavien Aké; Velusamy Srinivasan; Ryan T Novak; Lesley McGee; Cynthia G Whitney; Chris Van Beneden
Journal:  J Infect Dis       Date:  2019-10-31       Impact factor: 5.226
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