Literature DB >> 343109

Quantitative forward mutation assay in Salmonella typhimurium using 8-azaguanine resistance as a genetic marker.

T R Skopek, H L Liber, J J Krolewski, W G Thilly.   

Abstract

We have developed a quantitative forward mutation assay using Salmonella typhimurium, in which resistance to the purine analog 8-azaguanine is used as a genetic marker. We present the assay protocol, the concentration-dependent toxicity and mutagenicity of five known mutagens (N-methyl-N'-nitro-N-nitrosoguanidine, ICR-191, 9-aminoacridine, dimethylnitrosamine, and benzo[a]pyrene), and reconstruction experiments testing the assay for possible bias. The relative merits of forward versus reverse mutation assays are discussed.

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Year:  1978        PMID： 343109      PMCID： PMC411258          DOI： 10.1073/pnas.75.1.410

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  14 in total

1.  Detection of carcinogens as mutagens in the Salmonella/microsome test: assay of 300 chemicals.

Authors:  J McCann; E Choi; E Yamasaki; B N Ames
Journal:  Proc Natl Acad Sci U S A       Date:  1975-12       Impact factor: 11.205

2.  Mutations of Bacteria from Virus Sensitivity to Virus Resistance.

Authors:  S E Luria; M Delbrück
Journal:  Genetics       Date:  1943-11       Impact factor: 4.562

3.  Azaguanine-resistant mutants induced by several mutagens in a neurospora heterokaryon.

Authors:  G R Hoffmann; H V Malling
Journal:  Mutat Res       Date:  1975-03       Impact factor: 2.433

4.  9-Aminoacridine-A frameshift mutagen for Salmonella typhimurium TA 1537 inactive at the hgprt locus in human lymphoblasts.

Authors:  J G Deluca; J Krolewski; T R Skopek; D A Kaden; W G Thilly
Journal:  Mutat Res       Date:  1977-02       Impact factor: 2.433

5.  Purine phosphoribosyltransferases of Salmonella typhimurium.

Authors:  J Y Chou; R G Martin
Journal:  J Bacteriol       Date:  1972-11       Impact factor: 3.490

6.  An improved bacterial test system for the detection and classification of mutagens and carcinogens.

Authors:  B N Ames; F D Lee; W E Durston
Journal:  Proc Natl Acad Sci U S A       Date:  1973-03       Impact factor: 11.205

7.  Chemical carcinogens as frameshift mutagens: Salmonella DNA sequence sensitive to mutagenesis by polycyclic carcinogens.

Authors:  K Isono; J Yourno
Journal:  Proc Natl Acad Sci U S A       Date:  1974-05       Impact factor: 11.205

8.  Defective guanine uptake in an 8-azaguanine-resistant mutant of Salmonella typhimurium.

Authors:  J H Thakar; G P Kalle
Journal:  J Bacteriol       Date:  1968-02       Impact factor: 3.490

9.  Mutation of human lymphoblasts by methylnitrosourea.

Authors:  W G Thilly; J G DeLuca; H Hoppe; B W Penman
Journal:  Chem Biol Interact       Date:  1976-09       Impact factor: 5.192

10.  Genetic separation of hypoxanthine and guanine-xanthine phosphoribosyltransferase activities by deletion mutations in Salmonella typhimurium.

Authors:  J S Gots; C E Benson; S R Shumas
Journal:  J Bacteriol       Date:  1972-11       Impact factor: 3.490
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  24 in total

1.  Biodegradation of hexahydro-1,3,5-trinitro-1,3,5-triazine.

Authors:  N G McCormick; J H Cornell; A M Kaplan
Journal:  Appl Environ Microbiol       Date:  1981-11       Impact factor: 4.792

2.  Relative sensitivities of forward and reverse mutation assays in Salmonella typhimurium.

Authors:  T R Skopek; H L Liber; D A Kaden; W G Thilly
Journal:  Proc Natl Acad Sci U S A       Date:  1978-09       Impact factor: 11.205

3.  Urinary biological monitoring markers of anticancer drug exposure in oncology nurses.

Authors:  M A Newman; B G Valanis; R S Schoeny; S Q Hee
Journal:  Am J Public Health       Date:  1994-05       Impact factor: 9.308

4.  An immobilized microbial heparinase for blood deheparinization.

Authors:  R J Linhardt; C L Cooney; D Tapper; C A Zannetos; A K Larsen; R Langer
Journal:  Appl Biochem Biotechnol       Date:  1984-02       Impact factor: 2.926

5.  Appreciation of the value of different bacterial test systems for detecting and for ranking chemical mutagens.

Authors:  G R Mohn; J Ellenberger
Journal:  Arch Toxicol       Date:  1980-11       Impact factor: 5.153

6.  The apt/6-Methylpurine Counterselection System and Its Applications in Genetic Studies of the Hyperthermophilic Archaeon Sulfolobus islandicus.

Authors:  Changyi Zhang; Qunxin She; Hongkai Bi; Rachel J Whitaker
Journal:  Appl Environ Microbiol       Date:  2016-05-02       Impact factor: 4.792

7.  Aflatoxin B1 mutagenesis, DNA binding, and adduct formation in Salmonella typhimurium.

Authors:  A A Stark; J M Essigmann; A L Demain; T R Skopek; G N Wogan
Journal:  Proc Natl Acad Sci U S A       Date:  1979-03       Impact factor: 11.205

8.  Mollicellins: mutagenic and antibacterial mycotoxins.

Authors:  A A Stark; B Kobbe; D Matsuo; G Büchi; G N Wogan; A L Demain
Journal:  Appl Environ Microbiol       Date:  1978-09       Impact factor: 4.792

9.  Metabolically activated steviol, the aglycone of stevioside, is mutagenic.

Authors:  J M Pezzuto; C M Compadre; S M Swanson; D Nanayakkara; A D Kinghorn
Journal:  Proc Natl Acad Sci U S A       Date:  1985-04       Impact factor: 11.205

10.  Mutagenicity of islandicin and chrysophanol in the Salmonella/microsome system.

Authors:  D F Liberman; F L Schaefer; R C Fink; M Ramgopal; A C Ghosh; R Mulcahy
Journal:  Appl Environ Microbiol       Date:  1980-09       Impact factor: 4.792
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