Testing for COVID-19 in dental offices: Mechanism of action, application, and interpretation of laboratory and point-of-care screening tests.

BACKGROUND: The dental office potentially possesses all transmission risk factors for severe acute respiratory syndrome coronavirus 2. Anticipating the future widespread use of COVID-19 testing in dental offices, the authors wrote this article as a proactive effort to provide dental health care providers with current and necessary information surrounding the topic.
METHODS: The authors consulted all relevant and current guidelines from the Centers for Disease Control and Prevention and the US Food and Drug Administration, as well as online resources and review articles.
RESULTS: Routine COVID-19 screening and triage protocols are unable to detect all infected people. With the advancements in diagnostic tools and techniques, COVID-19 testing at home or in the dental office may provide dentists with the ability to evaluate the disease status of their patients. At-home or point-of-care (POC) tests, providing results within minutes of being administered, would allow for appropriate measures and rapid decisions about dental patients' care process. In this review, the authors provide information about available laboratory and POC COVID-19 screening methods and identify and elaborate on the options available for use by dentists as well as the regulatory requirements of test administration.
CONCLUSIONS: Dentists need to be familiar with COVID-19 POC testing options. In addition to contributing to public health, such tests may deliver rapid, accurate, and actionable results to clinical and infection control teams to enhance the safe patient flow in dental practices. PRACTICAL IMPLICATIONS: Oral health care must continue to offer safety in this or any future pandemics. Testing for severe acute respiratory syndrome coronavirus 2 at the POC offers a control mechanism contributing to and enhancing the real and perceived safety of care in the dental office setting.

The dental setting is a unique environment in the COVID-19 pandemic because it potentially possesses all transmission risk factors for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), as stated by the Centers for Diseases Control and Prevention (CDC). The infection occurs when the virus is inhaled or deposited on mucous membranes including the nose and mouth. As of April 2021, there are no direct data available to assess the risk of experiencing SARS-CoV-2 transmission in dental practice (that is, patient to patient, provider to patient, patient to provider, different procedures). Irrespective of the path of transmission, an estimated 0.9% of US dentists and an estimated 3.1% of US dental hygienists tested postive for SARS-CoV-2.

In view of the advancements in diagnostic and screening tools and techniques for COVID-19, dental practices may directly benefit from the ability to evaluate the disease status of patients while contributing to broader public health efforts. Rapid point-of-care (POC) tests provide results within minutes, allowing for appropriate measures and rapid decisions about next steps in the care process. SARS-CoV-2–induced COVID-19 represents only the latest manifestation of a 150-year history of epidemic disease patterns and transmission of contagious diseases. As such, the rapid screening and diagnostics testing approaches created or adapted for COVID-19 will be applicable for future viral pathogens, and thus, the dental office should be prepared to adopt standardized work flows as common future practice.

Anticipating the current and future need for pathogen testing in the dental offices, in this report we aim to provide the latest available laboratory methods for COVID-19 screening and diagnosis and to identify and further elaborate on the options available for use by dentists as well as the regulatory requirements of administration of those tests. Fundamentals of testing for COVID-19 detection is summarized in Figure 1 .

Figure 1

Fundamentals of COVID-19 testing. A. COVID-19 biomolecules can be detected in oral fluids as well as other sites and specimens. A detailed description and comprehensive review of these specimens' characteristics and viral loads and detection rates of COVID-19 is provided by Shirazi and colleagues.B. Schematic representation of detection window for COVID-19 biomarkers. The best detection and testing time for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA and antigen is up to 14 days from symptom onset. Immunoglobulin M (IgM) antibody against SARS-CoV-2 is detectable days after symptom onset, peaks during week 2, and will start to disappear at approximately week 4. Immunoglobulin G (IgG) against SARS-CoV-2 antibody will be detectable at approximately week 2 and persist for an unknown time. The duration of persistence of IgG antibody is not fully clear yet. C. COVID-19 biomarkers are detected with various laboratory methods that have different mechanism of action. Figure 2, Figure 3, Figure 4 include detailed description of each method. POC: Point-of-care.

Figure 2

Mechanism of action of nucleic acid amplification tests (NAATs). NAATs generally require 5 steps, which are modified or merged in different NAATs. The 5 steps are lysis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) viral particles in the sample; purification of the viral RNA; reverse transcription of the RNA to complementary DNA (cDNA), which produces DNA templates for the next step; thermocyclic or isothermal amplification of specific regions (target genes) of the cDNA using gene-specific primers, DNA polymerases, and probes to amplify only the selected region; and detection of the amplified cDNA, which can happen during or after step 4. The difference among various NAATs arise from modification of these steps. Some NAATs do not require separate lysis and purification steps, whereas some others merge reverse transcription and amplification. Analytical accuracy of COVID-19 NAATs depends mainly on the primer or probe design, and different assays use different primer or probe sets targeting different regions of the SARS-CoV-2 genome. Some NAATs, including isothermal methods, are authorized for point-of-care COVID-19 diagnostics and provide fast, sensitive, high-efficiency, and cost-effective results without need for specialized equipment.

Figure 3

Mechanism of action of antigen tests. Point-of-care antigen tests typically are lateral flow assays and come with a test strip or cassette composed of multiple overlapping pads. The clinical sample needs to be placed in a tube containing the extraction reagent after collection, which disrupts the viral particle and exposes the viral proteins. Next, the sample is added to the sample pad, where it starts to travel via capillary action through the conjugate pad and conjugates with the fluorescence-labeled severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) detection antibody or the detector-antibody gold conjugate. Next, it flows through an analytical membrane striped with a capture antibody specified as the test line. Most tests include a control line after the test line to validate proper fluid flow and the activity of assay reagents. The final outcome might be read via an instrument or displayed as colored lines for naked-eye reading.

Figure 4

Mechanism of action of antibody tests. The authorized point-of-care serologic tests commonly are based on lateral flow immunoassay, which detect binding antibodies. Lateral-flow immunoassays are composed of a cassette that encloses strip membranes. After the specimen is added to the sample pad, it moves forward by capillary action through the conjugated pad, where the present immunoglobulin G (IgG) and immunoglobulin M (IgM) antibodies in the sample interact with impeded gold conjugated severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antigens and rabbit-gold conjugated antibodies. The created antigen-antibody complexes move forward to the analytical membrane and bind antihuman IgG and IgM antibodies (capture antibodies) at the test line and get immobilized, whereas the rabbit-gold conjugate antibodies bind to antirabbit IgG antibodies at the control line and get immobilized. The result will be visible as colored lines owing to the accumulation of gold particles. Depending on whether the test is for IgM- or IgG-class antibodies or both, the test may show 1, 2, or 3 stripes.

Essential terminology to better understand and use COVID-19 resources

Screening testing

This type of testing is performed to identify infected people before development of symptoms or infected people without signs or symptoms (asymptomatic) or suspected exposure to COVID-19, who may be contagious to prevent the spread of virus. Examples of screening include testing plans developed by a workplace to test all employees returning to the workplace regardless of exposure or signs and symptoms. These tests are designed to have a higher false-positive rate (lower specificity) but high sensitivity (low false-negative rates) with the desire to screen for and capture those with the disease at the risk of involving those who are uninfected with more invasive testing.

Diagnostic testing

These types of tests also look for occurrence at the individual level but are performed when there is a particular reason to suspect that a person may be infected. They are performed when a person has signs or symptoms consistent with COVID-19, when a person is asymptomatic but has a recent known or suspected exposure to COVID-19, or when testing to determine resolution of infection. These are designed with high sensitivity (high true positive and low false negative) and high specificity (high true negative and low false positive) in populations considered at high risk of developing the disease. As implied, if the test is applied to a high-risk population, then a positive test will have a greater positive predictive value than the same result in a population in which there is a low disease prevalence (and thus a much higher rate of false-positive values).

Surveillance testing

This type of test is used to monitor population- or community-level outbreaks of COVID-19 or to characterize the incidence and prevalence of COVID-19. These are performed on deidentified specimens (typically, pooled), and the results are not linked to individual people. Thus, surveillance testing is used for health policy and not patient care. It may be a random sampling of a certain percentage of a specific population to monitor for increasing or decreasing prevalence and determining the population effect from community interventions such as social distancing. Table 1 provides a summarized comparison of different testing strategies.

Table 1

Comparison of different testing strategies for COVID-19.

CONSIDERATIONS FOR EACH STRATEGY	TESTING STRATEGY
	Screening	Diagnostic	Surveillance
For asymptomatic person without known or suspected exposure	Yes	No	NA∗
For symptomatic person or known or suspected exposure	No	Yes	NA
To characterize incidence and prevalence in the community	NA	NA	Yes
Results may be returned to people	Yes	Yes	No
Results are returned in aggregate to requesting institution	No	No	Yes
Results are reported to state public health department	Yes	Yes	If requested
Testing can be performed in a Clinical Laboratory Improvement Amendments-certified laboratory	Yes	Yes	Yes
Testing can be performed in a non Clinical Laboratory Improvement Amendments-certified laboratory	No	No	Yes
Test system must be Food and Drug Administration authorized or be offered under the policies in Food and Drug Administration’s guidance	Yes	Yes	No

NA: Not applicable.

Pretest probability

A Bayesian statistical approach uses the likelihood the person being tested really has the infection before the test, a test is performed, and the pre- and posttest probabilities are compared creating a likelihood index. Thus, a diagnostic test performs optimally for detecting an “infection” when the pretest probability is high (meaning that in the test population there is already a high rate of disease). The pretest probability increases with increasing prevalence in the population, and clinical indications or symptoms of illness in the person being tested. In contrast, tests typically perform best for excluding an infection when the pretest probability is low.

Diagnostic sensitivity and specificity

Sensitivity is the ability of a test to correctly identify people with infection and is the proportion of true positive results to the sum of true-positive and true-negative results. Specificity is the ability to correctly rule out infection and is the proportion of truenegative results to the sum of true-negative and false-positive results. False-negative results are the most consequential, and thus screening tests are based to capture those at risk.

Positive and negative percentage agreement

In new situations like the COVID-19 pandemic in which there is no clinical reference standard test with 100% sensitivity and specificity, the sensitivity and specificity of a new test will be evaluated against an imperfect reference standard and will be respectively reported as positive percentage agreement (the proportion of people with the target condition by the imperfect reference standard who test positive) and negative percentage agreement (the proportion of people free of the target condition by imperfect reference standard who test negative). The challenge is that the benchmark for the positive percentage agreement will change over time, so this is at best a field measure supporting just-in-time clinical decision making.

Analytical sensitivity

Analytic sensitivity is also referred to as limit of detection and is the minimum amount of SARS-CoV-2 viral particle count detected or quantitatively determined greater than 95% of the time by means of the used test.

Analytical specificity

Analytic specificity is the ability of a specific test platform to detect its intended target (that is, SARS-CoV-2). Cross-reactivity and target interference affect analytical specificities. Cross-reactivity occurs when a patient’s sample contains SARS-CoV-2 as well as other genetically related organisms, and the assay’s analytic reagents (for example, polymerase chain reaction primers or antibodies) cross-react or anneal to sequences or antigens of these organisms, generating a false-positive results. Interference occurs when the specimen is contaminated with endogenous elements, such as hemoglobin or medications, or exogenous inhibitory substances, such as lipstick, hand cream, glove powder, and serum separators. These inhibit chemical and biochemical reactions essential to the test and increase false-negative results.

Positive predictive value

Positive predictive value is the probability a patient with a positive test result most likely has the infection (or the test target). It is affected by general prevalence, test sensitivity, and pretest probability in the population of interest. In a high-prevalence setting, the positive predictive value increases, meaning it is more likely people testing positive are truly positive for disease (or appropriately reactive in the case of an antibody test platform). In turn, as prevalence in the population declines, false-positive rates will increase as they are inversely related. Test specificity and pretest probability have the greatest impact on false-positive rates. As the pretest probability and the specificity of the test increases, the false-positive rate decreases and the positive predictive value increases. When a test is used in a population in which prevalence is low, the positive predictive value drops because there are more false-positive results, since the pretest probability is low.

Negative predictive value

Negative predictive value is the possibility that a patient with a negative test result most likely does not have the infection. It is affected by pretest probability and prevalence. In a high-prevalence setting, the negative predictive value declines, whereas in a low-prevalence setting it increases. Pretest probability and test sensitivity have the greatest impact on false-negative rates. As the pretest probability decreases, the false-negative rate decreases and the negative predictive value increases. As the sensitivity of the test increases, the false-negative rate decreases and the negative predictive value increases. Table 2 shows the relationship between test characteristics and test outcome.

Table 2

Crosstalk between test characteristics and test results.

PRETEST PROBABILITY∗	or	POSITIVE PREDICTIVE VALUE†	or	NEGATIVE PREDICTIVE VALUE†	OUTCOME
Low		Low		High	Increased chance of true-negative or false-positive results
High		High		Low	Increased chance of true-positive or false-negative results

Specificity and sensitivity of tests are not affected by the pretest probability.

Pretest probability affects predictive values.

Methods

We reviewed the CDC guidelines on COVID-19 for up-to-date (as of April 5, 2021) information regarding testing options and related matters. , We consulted the US Food and Drug Administration (FDA) website for the latest authorized in vitro diagnostic tests.7, 8, 9 We searched PubMed (MEDLINE) for review articles regarding mechanism of action of different testing options.

Alternative Methods for Laboratory and POC Detection of SARS-CoV-2

Laboratory testing for SARS-CoV-2 involves detection of the viral RNA using nucleic acid amplification tests (NAATs), of the viral structural (or nucleocapsid) proteins using antigen tests or of the virus-specific human adaptive immune response (antibodies) using immunoassays ( Figure 1C, Figure 2, Figure 3, Figure 4 ). An optimum test for direct diagnosis of SARS-CoV-2 infection must have low infrastructural requirements, short turnaround time, minimum batching, high throughput, high accuracy, and low cost to allow public access. Table 3 summarizes the important aspects of each testing method.

Table 3

Comparison of different testing options for COVID-19.

DESCRIPTION	NUCLEIC ACID AMPLIFICATION TEST	ANTIGENT TEST	ANTIBODY TEST
Test Type	Viral, molecular	Viral, immunoassay	Serologic, immunoassay
Purpose	Diagnostic	Diagnostic, screening	Screening
Intended Use	Detect current infection	Detect current infection	Detect prior or recent infection
Analyte Detected	Viral RNA	Viral structural proteins	Virus-specific antibodies
Sensitivity	Generally high	Moderate to high	Moderate to high
Specificity	High	High	High
Test Complexity	Differs by test	Relatively easy to use	Relatively easy to use
Turnaround Time	15 min to > 2 d	15 min to > 2 d	15 min to > 2 d
Operating Temperature	Need thermal cycling—some are isothermal	Ambient	Ambient
Results Presentation	Quantitative or qualitative	Visual or instrument read	Visual or instrument read
Authorized for Point-of-careUse	Some tests	Most tests	Some tests
Authorized for Use at Home	Very few, sample collection is possible at home for many tests	Some tests	No
Suggested Test Time	1 d after infection to 14 d from symptom onset	5-7 d from symptom onset	≥ 15 d from symptom onset
Cost per Test	Moderate (≈ $100-$200)	Low (≈ $5-$50)	Moderate (≈ $100-$200)
Authorized Specimen Type	Nasopharyngeal, oropharyngeal, nasal, sputum, saliva	Nasopharyngeal, nasal	Whole blood, serum, plasma, fingerstick blood
Transport Medium	Viral transport medium or saline—nothing needed for some tests	Extraction medium	Anticoagulant—nothing needed for fingerstick tests
Disadvantages	High requirements for operators and laboratory conditions. High cost, complexity, and turnaround time	Less sensitive in the early stage of disease	Lower sensitivity and specificity. Cross-reactivity. Long window period

Molecular diagnostic tests

The recommended test platform for diagnosis of SARS-CoV-2 infection involves detection of viral RNA using NAATs. The FDA-authorized methods in this category use a variety of platforms. A detailed description of NAAT-based techniques has been widely discussed in the literature.11, 12, 13, 14, 15, 16, 17, 18 Figure 2 shows a simplified and general process of NAATs.

A single negative NAAT result does not exclude infection, and factors such as exposure risk and potential laboratory errors must be considered. False-negative results may occur if the specimen was obtained too early after exposure or was not collected, transported, handled, or treated properly; if the nucleic acid extraction step was improper; or if the sample contained amplification inhibitors or an insufficient amount of virus. The sensitivity of NAATs depends greatly on the sample type (that is, nasopharyngeal, saliva), stage of the infection, viral load, and time of collection in relation to the course of disease. , Figure 1B depicts the suggested time for the use of different tests.

A positive result is only indicative of the presence of SARS-CoV-2 RNA (including degraded viral RNA) and not an infectious (contagious) state. The viral RNA may be detectable for several days to several weeks after the entry of live virus. Assays using droplet digital polymerase chain reaction (PCR), a method enabling high throughput and absolute quantification of rare targets within a biological sample, detect intact SARS-CoV-2 RNA and can detect potentially infectious clinical COVID-19 samples. A false-positive result may occur in which the pretest probability or the prevalence of infection is low, or the false-positive result may be due to technical errors, the cross-reactivity of primers, cross-contamination of the sample during its handling or preparation, or contamination of reagents in the laboratory. , , 19, 20, 21 Thus, clinical correlations with signs and symptoms, patient history, days from symptom onset, and relevant diagnostic information are necessary to evaluate a patient's infection status and risk. Nevertheless, the infectivity period has been established at 10 days from the first positive molecular test in asymptomatic patients or an additional 3 days without symptoms in patients with clinical manifestations. None of the COVID-19 vaccines authorized or in development as of April 2021 will result in a positive result in NAATs because they do not contain live virus or its RNA fragments.

Antigen diagnostic tests

Antigen tests directly detect viral structural proteins without any amplification steps and do not require trained personnel and special laboratory instruments or reagents. The tests use antibodies reactive to SARS Co-V-2 proteins to detect infection. Although they are rapid, simple, and relatively inexpensive immunoassays, they only reveal the active, current COVID-19 infection and level of contagiousness possibility. A description of methods for antigen detection has been described in the literature.14, 15, 16, 17, 18

The authorized antigen tests are performed only on nasopharyngeal or nasal swab specimens. They are not restricted to specific age groups, and many can be used at POC with results in about 15 minutes. They can be used in screening or diagnostic testing situations in which a person is suspected for COVID-19 or has had a known exposure to someone with COVID-19. They also can be used as screening tests on asymptomatic people to detect or exclude COVID-19 or to determine whether a person who previously was diagnosed with COVID-19 remains infectious. Screening testing with antigen tests is also beneficial when repeat testing could identify quickly people with a SARS-CoV-2 infection, thus alerting health care workers to institute prevention and control measures. There is value in providing immediate results with antigen tests when a rapid test turnaround time is required, even though they may have lower sensitivity than NAATs. Figure 3 describes a simplified process of antigen tests.

Inherently, antigen-screening platforms are less sensitive than genetic PCR-based detection platforms (for example, NAATs) but have a similarly high specificity. They are less reliable in the clinical diagnosis of COVID-19 patients with low viral load. The antigen test’s best clinical performance is during early symptomatic infection, when the viral load is considered to be the highest (Figure 1B). ,

One limitation of antigen testing is the potential for higher false-negative results. False-negative results occur owing to inherent low sensitivity, if the antigen level in specimens collected is below the detection limit of the assay, or if the time of collection is before or much later after symptom onset. Also, the stability of antigens in biological samples can degrade as a function of time and temperature after sample collection. Because positive and negative predictive values are highly dependent on communal COVID-19 prevalence, false-negative test results are statistically more likely during peak activity when prevalence of disease is high. Negative antigen test results should be handled differently depending on the test, its stated performance characteristics, and intended application (for example, clinical diagnosis, screening). In general, negative antigen test results should be considered presumptive, meaning that they are preliminary results. Thus, an antigen test result should be confirmed with an NAAT, especially if the result of the antigen test is inconsistent with the clinical context. More information is provided in the Appendix (available online at the end of this article).

False-positive test results are unlikely when an antigen test is used according to the manufacturer’s instructions, given that the specificity of antigen tests is generally as high as most NAATs. However, antigen tests detect both viable and nonviable SARS-CoV-2 viral antigens and may yield a positive result in the absence of live virus (as is the case with the NAATs). The potential for a false-positive test result is more likely when the prevalence of infection or pretest probability is low or if samples are cross-contaminated. Nevertheless, clinicians can rely on a positive antigen test result for a symptomatic patient. None of the COVID-19 vaccines authorized (or in development) will cause a positive result in antigen tests because they do not contain viral antigens. Table 4 summarizes the relationship between the results of antigen tests, NAATs, and clinical manifestation of COVID-19. An antigen testing algorithm based on the recommendation of the CDC is provided in the Appendix (available online at the end of this article).

Table 4

Relationship between the results of antibody tests, antigen tests, NAATs,∗ and clinical manifestation of COVID-19.

CLINICAL MANIFESTATION	NAAT RESULT	ANTIGEN TEST RESULT	SEROLOGIC TEST RESULT		RISK OF EXPERIENCING INFECTION TRANSMISSION
			Immunoglobulin M	Immunoglobulin G
Early Stage of Infection	+	+	+	−	High
Active Phase of Infection	+	+	+	+	High
Late Stage of Infection, Possible Recurrent Infection	+	+	−	+	High
Window Period	+	+	−	−	High
Early Stage of Infection, False-Negative NAAT Result	−	+	+	−	High
Late Stage of Infection, False-Negative NAAT Result	−	+	−	+	High
Window Period, False-Negative NAAT Result	−	+	−	−	High
Asymptomatic Infection, False-Positive NAAT Result, False-Positive SerologicTest Result	+	−	+	+	Low
Window Period with Asymptomatic Infection, False-Positive NAAT Test Result	+	−	−	−	Low
Past Infection	−	−	−	+	Low
Convalescence	−	−	+	+	Low

NAAT: Nucleic acid amplification test.

Dental offices must follow the manufacturer’s instructions and package inserts when using these tests to obtain valid and reliable results. The performance of antigen tests can be affected if the test components are not stored and handled properly. They should remain in the sealed pouch until immediately before use, never be frozen, and always be brought to room temperature (15-30 °C) before use. The appropriate incubation time before reading the results and whether the results should be interpreted visually or with an instrument analyzer should be considered. Reading the test before or after the specified time could result in false-positive or false-negative test results. More information regarding antigen tests is provided in the Appendix (available online at the end of this article).

Serologic tests

Serologic tests use antibody-antigen recognition by using cloned or recombinant SARS-CoV-2 antigens to detect patient antibodies generated against the virus. Serologic tests authorized for emergency use by the FDA detect immunoglobulin M (IgM), immunoglobulin G (IgG), or total antibody. A detailed description of these techniques is reported elsewhere. , , , Figure 4 depicts a simplified process of antibody detection tests.

FDA-authorized serologic tests are performed using whole venous or fingerstick blood, serum, or plasma, with comparable sensitivity and specificity between whole blood and serum. They are not costly or restricted to any certain age, are relatively easy to use, and generally return results in approximately 15 through 90 minutes depending on the assay, some of which may require laboratory technicians and specialized instruments. Almost all the tests are qualitative (providing a result that is positive, negative, or indeterminate) rather than quantitative (providing a quantitative assessment of antibody levels). Some serologic tests are available for POC use and use lateral flow immunoassay with fingerstick blood collection. Serologic testing, by itself, should not be used to establish the presence or absence of SARS-CoV-2 infection or reinfection. COVID-19 vaccines might result in positive antibody test results because they trigger generation of antibody against SARS-CoV-2.

Serologic tests are both less sensitive and less specific than NAATs and most antigen tests must contend with the inherent variability of the human polyclonal antibody response. In addition, they inherently measure a response to an infection, not the infection itself. The delay required for plasma B cells to generate a humoral antibody response and for the body to mount a full polyclonal humoral immunity does not allow for the use of serologic tests early in the course of infection. , Serologic tests can be used to determine different aspects of immune response and functionality of antibodies. They also are indicated in epidemiologic and surveillance studies to mass screen the prevalence of COVID-19 and identify asymptomatic patients or carriers and to detect the presence, nature, and extent of the humoral immune response against SARS-CoV-2 infection, which also allows for differentiating between past and current infection as well as its progress. Serologic tests are also useful in vaccine studies and investigation of late complications, whereas NAATs may yield false-negative results owing to low viral loads in the tested samples. They also can be offered in adjunction to NAATs or antigen tests for people who seek treatment 9 through 14 days after illness onset, during which the sensitivity of NAATs is decreasing and the sensitivity of serologic testing is increasing. , , , , Table 4 summarizes the relationship between the results of antibody tests, NAATs, and clinical manifestation of COVID-19. The immunologic response to SARS-CoV-2 infection is not limited to antibody production; the cellular responses of T cells and macrophages against the invading virus may play a significant, yet currently underexamined, role in disease progression. Potential cellular assessments may prove to be of additional value.

As of April 2021, data are limited regarding the nature, kinetics, and dynamics of immunologic responses to SARS-CoV-2 infection, which can vary with age, sex, and presence of comorbidities, as well as characteristics of the viral load and variant infectivity and transmissibility. It has been suggested that seroconversion after exposure to SARS-CoV-2 is similar to other acute viral infections, with IgG concentration beginning to rise as IgM concentration reaches a plateau and then declines. IgM usually becomes undetectable weeks to months after infection, whereas IgG may remain detectable for months or years (Figure 1B). Therefore, IgM and IgG are indicative of early or recent and late or past stage responses to the infection. Nevertheless, the role of virus-specific immunoglobulin A (IgA) against SARS-CoV-2 infection in humans should be emphasized. , , It is suggested that IgA-mediated mucosal immunity is a critical defense mechanism against SARS-CoV-2 at the individual level, which may reduce infectivity of human secretions and viral transmission. Secretory IgA can be measured easily in saliva. This also may encourage the development of vaccines that induce specific mucosal IgA responses against SARS-CoV-2. In addition, it provides the chance to test for antibodies in samples, including saliva, respiratory tract secretions, and other body fluids in addition to blood products. More information regarding serologic tests is provided in the Appendix (available online at the end of this article).

The timing of sample collection, host immune factors, and test characteristics should be considered when interpreting antibody test results. There is a possibility of false-negative results and false-positive results considering the overall sensitivity and specificity of antibody tests. Negative antibody results do not exclude SARS-CoV-2 infection, and false-negative results may be associated with the time needed to develop an immune response early during the course of disease (window period). Some people do not develop detectable IgG or IgM antibodies after infection. Chronic immunosuppression may cause an impaired immune response and no detectable titer. Alternatively, false-positive cross-reactivity may result in patients who have been infected with related human coronaviruses before, such as those that cause the common cold. , , ,

What dentists need to know about point-of-care testing in the dental office

The American Dental Association (ADA) considers POC testing and screening of patients for medical conditions, including COVID-19, to be within a dentist’s scope of practice if such conditions could complicate oral health care or put the patient and dental staff at risk of developing the disease. The FDA includes dentists among those professionals who can test for COVID-19, and the CDC recommends dental facilities to consider implementing preprocedure testing for COVID-19. ,

The ADA has released the COVID-19 & Lab Testing Requirements Toolkit to help guide dentists interested in offering their patients rapid-response, POC COVID-19 screening and testing within their practices. It provides information for dentists on applying for the federal certification required to offer this type of testing. The toolkit also includes compliance requirements, tips for developing a waived testing program in dental practices, COVID-19 test reporting requirements, and guidance on medical benefit plan claim filing for in-office COVID-19 testing. Any dental practice that plans to incorporate testing for COVID-19 into their screening protocol is considered a clinical laboratory and must comply with regulations set by the Clinical Laboratory Improvement Amendments (CLIA) law, which establishes quality standards for all laboratory testing to ensure accuracy, reliability, and timeliness of patient test results regardless of where the test was performed. , , Specific requirements for each state and guidance on patient medical benefit plan claim filing for in-office COVID-19 testing are provided in the ADA toolkit.

Dentists who register for a CLIA certificate of waiver for their practices must only perform tests that have been classified as waived tests, which include some NAATs and antigen and antibody tests, and must follow the manufacturer’s instructions for each test. , The CDC has provided comprehensive guidelines and useful educational materials for POC testing and infection control. , , According to CDC reporting guidelines, dental offices with CLIA certificates are considered testing sites and must report both the positive and negative results of COVID-19 diagnostic and screening tests that they perform using in-home diagnostic or screening tests to the appropriate state or local public health department. , CDC guidelines must be followed for specimen collection and handling of POC tests. ,

The POC testing options and technologies for COVID-19 testing are growing and evolving fast. As of April 2021, multiple POC testing options are authorized, and dental offices with CLIA certificate are eligible to use and benefit from them. However, there are no saliva-based POC antigen or antibody tests approved for use. The characteristics of all authorized POC tests for COVID-19 are summarized in eTable 1, eTable 2, eTable 3 (available online at the end of this article). Large-scale assessment of the sensitivity and specificity of these tests are not reported widely.

eTable 1

Available POC∗ molecular diagnostic tests for COVID-19.†

PRODUCT‡	TEST AT HOME	ACCESSIBILITY	MULTIANALYTE	METHOD	RESULTS REPRESENTATION§	SAMPLE TYPE¶	TARGET	PERFORMANCE EVIDENCE FOR SARS-CoV-2#	TIME TO RESULSTS
Cue COVID-19 Test for Home and OTC∗∗Use	Yes	OTC home testing, health care provider†† at POC	No	RT,‡‡ isothermal amplification	Qualitative, instrument read	Anterior nasal swab in adults (self-swabbing) or children ≥ 2 y of age (swabbed by an adult)	Nucleocapsid gene	LoD§§: 20 genome copies/sample wandPPA¶¶: 97.4% (95% CI, 86.5% to 99.5%)NPA##: 99.1% (95% CI, 96.9% to 99.8%)	~ 20 min
Visby Medical COVID-19 POC Test	No	Health care provider	No	RT-PCR∗∗∗	Qualitative, visual read	Nasopharyngeal, anterior nasal, or Midturbinate swabs collected by a health care provider or anterior nasal or mid-turbinate swabs self-collected (by people aged ≥ 18 y, under the supervision of a health care provider)	Nucleocapsid gene	LoD: 435 copies/swabPPA: 100.0% (95% CI, 89.0% to 100.0%)NPA: 95.3% (95% CI, 87.1% to 98.4%)	~ 30 min
Xpert Xpress SARS-CoV-2 Test	No	Health care provider	No	Real-time RT-PCR	Qualitative, instrument read	Nasopharyngeal, nasal, or mid-turbinate swab	Nucleocapsid gene, envelope protein gene	LoD: 5.4 x 103 RNA NAAT‡‡‡ detectable units/milliliterPPA: 97.8% (95% CI, 88.4% to 99.6%)NPA: 95.6% (95% CI, 85.2% to 98.8%)	30 min for positives and ~ 45 min for negatives
Accula SARS-Cov-2 Test	No	Health care provider	No	PCR and lateral flow	Qualitative, visual read	Clinician-collected nasal or nasal mid-turbinate swab samples or clinician-instructed self-collected (collected on site) nasal swab	Nucleocapsid gene	LoD: 200 copies/reaction.PPA: 95.8% (95% CI, 78.88% to 99.89%)NPA: 100% (95% CI, 86.77% to 100%)	~ 30 min
BioFire Respiratory Panel 2.1-EZ	No	Health care provider	Yes,16 viruses and 4 bacteria	Real-time, nested multiplexed PCR	Qualitative, instrument read	Nasopharyngeal swab	Spike protein gene and membrane protein gene	Overall, 97.1% sensitivity and 99.3% specificity (prospective specimens)98.0% sensitivity and 100% specificity (archived specimens)100% PPA and 100% NPA (contrived specimens)	~ 45 min
Cobas SARS-CoV-2 & Influenza A/B Nucleic Acid Test	No	Health care provider	SARS-CoV-2, influenza A and influenza B virus	Multiplexed real-time RT-PCR	Qualitative, instrument read	Health care provider-collected nasopharyngeal and nasal swabs and self-collected nasal swabs (collected in a health care setting with instruction by a health care provider)	RdRp gene (ORF1ab) and nucleocapsid gene	LoD: 12 copies/mLPPA: 100%NPA: 100%	~ 20 min
Lucira COVID-19 All-in-One Test Kit	Yes	Prescription home testing, health care provider at POC	No	RT-loop mediated isothermal amplification	Qualitative, instrument read	Self-collected nasal swab in people aged ≥14 y and in people aged ≤ 13 y when the specimen is collected by a health care provider at the POC	2 non overlapping regions of the nucleocapsid gene	LoD: 900 copies/mLPPA: 94.1% (95%CI, 85.5% to 98.4%NPA: 98.0% (95%CI, 89.4% to 99.9%)	< 30 min
ID NOW COVID-19	No	Health care provider	No	RT, isothermal amplification	Qualitative, instrument read	Direct nasal, nasopharyngeal or throat swabs	RdRp	LoD: 125 genome equivalents/mL	≤ 13 min
Xpert Xpress SARS-CoV-2/Flu/RSV†††	No	Health care provider	SARS-CoV-2, influenza A and influenza B virus, RSV	Multiplexed real-time RT-PCR	Qualitative, instrument read	Nasopharyngeal or nasal swab	Envelope and N-nucleocapsid (N2)	LoD:131 copies/mL for COVID-19PPA and NPA of 97.9% and 100.0% for SARS-CoV-2, respectively;100.0% and 100.0% for influenza A, respectively; 100.0% and 99.0% for influenza B, respectively100.0% and 100.0% for RSV, respectively	25 min for positive COVID-19 results and 36 min for all 4 pathogens
Cue COVID-19 Test	No	Health care provider	No	RT, isothermal amplification	Qualitative, instrument read	Direct nasal swabs	Nucleocapsid gene	LoD: 1,300 copies/mLPPA 100%NPA 92%	25 min

POC: Point-of-care.

As of April 5, 2021. An up-to-date list of POC molecular diagnostic tests is available online at the US Food and Drug Administration’s “In Vitro Diagnostics EUAs: Molecular Diagnostic Tests for SARS-CoV-2.” Those tests that have W in the Authorized Setting column are authorized for POC use. The link for each product’s user instruction is provided in the Authorization Documents section.

Products are listed according to the authorization date.

All of the mentioned products automate all aspects of nucleic acid testing, from nucleic acid extraction to results.

Nasal and nasopharyngeal swabs are contraindicated in people who are susceptible to nosebleeds or have had recent facial or head injury or surgery.

SARS-CoV-2: Severe acute respiratory syndrome coronavirus 2.

OTC: Over-the-counter.

A health care provider includes, but is not limited to, physicians, dentists, nurses, pharmacists, technologists, laboratory directors, epidemiologists, or any other practitioners or allied health care professionals. The Centers for Disease Control and Prevention provides an online guideline for the collecting and handling of clinical specimens for COVID-19 testing.

RT: Reverse transcription.

LoD: Limit of detection.

PPA: Positive percentage agreement.

NPA: Negative percentage agreement.

PCR: Polymerase chain reaction.

NAAT: Nucleic acid amplification test.

RSV: Respiratory syncytial virus.

eTable 2

Available POC∗ antigen diagnostic tests for COVID-19.†

PRODUCT‡	TEST AT HOME	ACCESSIBILITY	MULTIANALYTE	METHOD	RESULTS READING	SAMPLE TYPE§	TAREGET ANTIGEN¶	PERFORMANCE EVIDENCE FOR SARS-CoV-2#	TIME TO RESULTS	SUGGESTED TIME POINT FOR TESTING
BD Veritor System for Rapid Detection of SARS-CoV-2 & Flu A+B	No	Health care provider ∗∗	Yes	Chromatographic digital immunoassay	Instrument	Direct anterior nasal swab	SARS-CoV-2 nucleocapsid antigen or influenza A and B nucleoprotein	LoD††: 2.8 x 102 TCID50‡‡/milliliterPPA§§: 86.7% (95% CI, 75.8% to 93.1%)NPA##: 99.5% (95% CI 97.4-99.9%)	20 min	Within the first 6 d of symptom onset
QuickVue At-Home COVID-19 Test	Yes	Prescription home testing, health care provider	No	Lateral flow fluorescence immunoassay	Visual	Direct anterior nares swab	Nucleocapsid protein	LoD: 1.91 x 104 TCID50/mLPPA: 84.8% (95% CI, 71.8% to 92.4%)NPA: 99.1 % (95% CI, 95.2% to 99.8%)	10 min	Within the first 6 d of the onset of symptoms
Ellume COVID-19 Home Test	Yes	Over-the-counter home testing, health care provider	No	Lateral flow fluorescence immunoassay	Instrument	Midturbinate nasal swabs that are self-collected by people aged ≥ 16 y, or are collected by an adult from people aged ≥ 2 yr	Nucleocapsid protein	LoD:103.80 TCID50/mLPPA: 95% (95% CI, 82% to 99%)NPA: 97% (95% CI, 93% to 99%)	20 min	Intended for use in people with or without symptoms or other reasons to suspect a COVID-19 infection
LumiraDx SARS-CoV-2 Ag Test	No	Health care provider	No	Microfluidic immuno-fluorescence assay	Instrument	Direct nasal swab	Nucleocapsid protein	LoD: 32 TCID50/mLPPA: 97.6% (95% CI, 91.6% to 99.3%)NPA: 96.6% (95% CI, 92.7% to 98.4%)	12 min	Within the first 12 days of symptom onset
BD Veritor System for Rapid Detection of SARS-CoV-2	No	Health care provider	No	Chromatographic digital immunoassay	Instrument	Nasal swabs	Nucleocapsid protein	LoD: 140 TCID50/mLPPA: 84% (95% CI, 67% to 93%)NPA: 100% (95% CI, 98% to 100%)	15 min	Within the first 5 d of onset of symptoms
QuickVue SARS Antigen Test	No	Health care provider	No	Lateral flow immunoassay	Visual	Anterior nares swab	Nucleocapsid protein	LoD: 7.57x103 TCID50/mLPPA: 96.6% (95% CI, 88.3% to 99.0%)NPA: 99.3% (95% CI, 96.0% to 99.9%)	10 min	Within the first 5 d of the onset of symptoms
BinaxNOW COVID-19 Ag Card Home Test	Yes	Over-the-counter home testing; Healthcare provider	No	Lateral flow immunoassay	Visual	Self-collected observed direct anterior nares swab samples from people aged ≥ 15 y.Adult-collected nasal swab samples from people aged ≥ 4 y.	Nucleocapsid protein	LoD: 140.6 TCID50/mLPPA: 84.6% (95% CI, 76.8% to 90.6%)NPA: 98.5% (95% CI, 96.6% to 99.5%)	15 min	Within the first 7 d of symptom onset
Clip COVID Rapid Antigen Test	No	Health care provider	No	Lateral flow immune-luminescent assay	Instrument	Direct anterior nasal swab	Nucleocapsid protein	LoD: 88 TCID50/mLPPA: 96.9% (95% CI, 83.8% to 99.9%)NPA: 100% (95% CI, 97.3% to 100%)	30 min	Within the first 5 d of onset of symptoms
CareStart COVID-19 Antigen Test	No	Health care provider	No	Lateral flow immunoassay	Visual	Nasopharyngeal swab specimens	Nucleocapsid protein	LoD: 6.4 x 103 TCID50/mLPPA: 83.33% (95% CI, 43.65% to 97.00%).NPA: 100% (95% CI, 81.57% to 100%)	10 min	Within the first 5 d of onset of symptoms
Sofia 2 Flu + SARS Antigen FIA	No	Health care provider	Yes	Lateral flow fluorescence immunoassay	Instrument	Direct nasopharyngeal and nasal swab	Nucleocapsid protein from SARS-CoV-2, influenza A, and influenza B	LoD: 91.7 x 103 TCID50/mLPPA: 95.2% (95% CI, 84.2% to 98.7%)NPA: 100.0% (95% CI, 96.9% to 100.0%)	15 min	Within the first 5 d of onset of symptoms
Sofia SARS Antigen FIA	No	Health care provider	No	Lateral flow fluorescence immunoassay	Instrument	Direct nasopharyngeal and nasal swab	Nucleocapsid protein	LoD: 113 TCID50/mLPPA: 96.7% (95% CI, 83.3% to 99.4%)NPA: 100.0% (95% CI, 97.9% to 100.0%)	15 min	Within the first 5 d of onset of symptoms

POC: Point-of-care.

As of April 5, 2021. An up-to-date list of POC antigen diagnostic tests is available online at the US Food and Drug Administration’s “In Vitro Diagnostics EUAs: Antigen Diagnostic Tests for SARS-CoV-2.” Those tests that have W in the Authorized Setting column are authorized for POC use. The link for each product’s user instruction is provided in the Authorization Documents section.

Products are listed according to the authorization date.

Nasal and nasopharyngeal swabs are contraindicated in people who are susceptible to nosebleeds or have had recent facial or head injury or surgery.

None of the listed tests differentiate between severe acute respiratory syndrome coronavirus (SARS-CoV) and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Possible false-positive results in patients with history of infection with SARS-CoV-2.

Failure of the user to follow the test procedure correctly may affect the test performance adversely or invalidate the test result. All results are presumptive in asymptomatic people. Positive results indicate the presence of viral antigens, but the clinical correlation with medical history and other diagnostic information are necessary to determine infection status. Negative results are presumptive in symptomatic people.

A health care provider includes, but is not limited to, physicians, dentists, nurses, pharmacists, technologists, laboratory directors, epidemiologists, or any other practitioners or allied health care professionals. The Centers for Disease Control and Prevention provides an online guideline for the collecting and handling of clinical specimens for COVID-19 testing.

LoD: Limit of detection.

TCID50: Median tissue culture infectious dose.

PPA: Positive percentage agreement.

NPA: Negative percentage agreement.

eTable 3

Available POC∗ serologic tests for COVID-19.†

PRODUCT‡	TEST AT HOME	ACCESSIBILITY	METHOD	RESULTS READING	SAMPLE TYPE	TAREGET ANTIBODY§	PERFORMANCE EVIDENCE FOR SARS-CoV-2¶	TIME TO RESULSTS
MidaSpot COVID-19 Antibody Combo Detection Kit	No	Health care provider#	Lateral flow	Visual	Fingerstick whole blood	IgM and IgG	IgM NPA∗∗: 97.7% (95% CI,†† 91.8% to 100%)IgG NPA: 100% (95% CI, 92.0% to 100%)8-14 dIgM PPA‡‡: 100.0% (95% CI, 63.1% to 100.0%)IgG PPA: 75% (95% CI, 40.9% to 92.7%)≥ 15 dIgM PPA: 100.0% (95% CI, 88.4% to 100.0%)IgG PPA: 100% (95% CI, 88.6% to 100%)	20 min
RapCov Rapid COVID-19 Test	No	Health care provider	Lateral flow	Visual	Fingerstick whole blood	IgG	PPA: 90.0% (95% CI, 73.6% to 97.3%)NPA: 95.2% (95% CI, 89.2% to 97.9%)	15 min
Sienna-Clarity COVIBLOCK COVID-19 IgG/IgM Rapid Test Cassette	No	Health care provider	Lateral flow	Visual	Fingerstick whole blood	IgM and IgG	Multiple clinical performance data. See the instructions for use. §§	10 min
RightSign COVID-19 IgG/IgM Rapid Test Cassette	No	Health care provider	Lateral flow	Visual	Fingerstick whole blood	IgM and IgG	IgM PPA: 88.89% (95% CI, 75.95% to 96.29%)IgM NPA:100.00% (95% CI, 91.06% to 100.00%)IgG PPA: 91.11% (95% CI, 78.78% to 97.52%)IgG NPA:100.00% (95% CI, 91.06% to 100.00%)	10 min
Assure COVID-19 IgG/IgM Rapid Test Device	No	Health care provider	Lateral flow	Visual	Fingerstick whole blood	IgM and IgGs	IgM NPA: 100% (95% CI. 97.7% to 100%)IgG NPA: 100% (95% CI, 97.7% to 100%)8-14 dIgM PPA: 83.3% (95% CI, 55.2% to 95.3%)IgG PPA: 83.3% (95% CI, 55.2% to 95.3%)≥ 15 dIgM PPA: 89.3% (95% CI, 72.8% to 96.3%)IgG PPA: 100% (95% CI, 91.2% to 100%)	15 min

POC: Point-of-care.

As of April 5, 2021. An up-to-date list of POC antigen diagnostic tests is available online at the US Food and Drug Administration’s “In Vitro Diagnostics EUAs: Serology and Other Adaptive Immune Response Tests for SARS-CoV-2.” Those tests that have W in the Authorized Setting column are authorized for POC use. The link for each product’s user instruction is provided in the Authorization Documents section.

Products are listed according to the authorization date.

Results from antibody testing should not be used to diagnose or exclude severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection or to inform infection status.

For optimal test performance, proper sample collection is critical. Failure to follow the procedure may give inaccurate results. In the early onset of symptom, anti-SARS-Cov-2 immunoglobulin M (IgM) and immunoglobulin G (IgG) antibody concentrations may be below detectable levels. SARS-CoV-2 IgG antibodies may be below detectable levels in patients who have been exhibiting symptoms for less than 15 days. Results from immunosuppressed patients should be interpreted with caution. False-positive results may be due to cross-reactivity with past or present infection with non-SARS-CoV-2 strains, such as coronaviruses HKU1, NL63, OC43, or 229E. Other information should be considered, including clinical history and local disease prevalence, in assessing the need for a second but different serology test to confirm an immune response.

A health care provider includes, but is not limited to, physicians, dentists, nurses, pharmacists, technologists, laboratory directors, epidemiologists, or any other practitioners or allied health care professionals. The Centers for Disease Control and Prevention provides an online guideline for the collecting and handling of clinical specimens for COVID-19 testing.

NPA: Negative percentage agreement.

CI: Confidence interval.

PPA: Positive percentage agreement.

Refer to product catalog for further information.

The FDA has issued an emergency use authorization for an over-the-counter and a prescription-only NAAT-based diagnostic tests that can be fully self-administered and provide rapid results at home, using nasal swab samples in people 2 years or older. They are also authorized for POC use, but samples must be collected by a health care provider. Many other NAAT-based assays also are authorized for at-home specimen collection with subsequent remote laboratory processing. There are some POC multiplexed reverse transcription PCR–based assays for simultaneous rapid and qualitative detection and differentiation of SARS-CoV-2, influenza A, and influenza B viruses in less than 1 hour. There is also an automated POC assay that uses isothermal nucleic acid amplification technology for qualitative detection of SARS-CoV-2 in direct (no need for transport medium) nasal, nasopharyngeal, and throat swabs in less than 13 minutes (eTable 1, available online at the end of this article).

Furthermore, the FDA has issued an emergency use authorization for an over-the-counter fully at-home diagnostic test for COVID-19, which is a lateral flow antigen test that uses a nasal swab sample from anyone 2 years or older and gives the result within 20 minutes. Other fully at-home antigen-based tests are also available with prescription. There are also antigen tests for simultaneous detection and differentiation of SARS-CoV-2, influenza A, and influenza B viruses, which give results within 15 minutes. This is in addition to multiple POC antigen tests that have been authorized for POC use (eTable 2, available online at the end of this article).

A number of serologic tests are authorized for POC use and detect IgG or IgM from fingerstick blood in approximately 10 to 20 minutes (eTable 3, available online at the end of this article).

These options, and particularly such tests that can be fully administered entirely outside of a laboratory or health care setting, may deliver rapid, accurate, and actionable results to dental clinical and infection control teams, with the promise of contributing to enhanced infection control in dental and other health care settings.

Discussion

The CDC has reported many people residing in the United States have seen a dentist over the past year but no other medical professional. Therefore, dental offices and settings could make a major contribution to public health by being the first-line of COVID-19 screening testing and vaccine advocacy for a large portion of the population. Dental settings may also be important for COVID-19 surveillance studies in the future.

Characteristics and detection rate or viral load of SARS-CoV-2 in different anatomic sites and from various specimens and fluids related to dentistry have been reviewed comprehensively in a 2021 article by Shirazi and colleagues. Lamberghini and colleagues reported an overall SARS-CoV-2 positivity rate of 2.3% in asymptomatic children attending a high-volume pediatric dental clinic. Conway and colleagues reported that the COVID-19 positivity rate was 0.6% (95% CI, 0.4% to 0.8%) in child and adult asymptomatic patients attending multiple oral health care centers. Palla and Callahan reported 6.7% of asymptomatic patients attending an emergency department tested positive for COVID-19, 50% of whom visited a dentist within 1 week of consultation.

These findings highlight that basic patient triage protocols alone would not identify everyone who is infected. Therefore, timely, accurate (highly sensitive and specific), and rapid screening and testing that can distinguish people with COVID-19 from those who are healthy or infected with other viruses is an essential need to take required actions, optimize patient care, maintain the safety of dental patients and treatment providers, and contain and prevent disease spread. POC tests that can be fully administered entirely outside of a laboratory or health care setting will deliver rapid, accurate, and actionable results to dental clinical and infection control teams and will contribute greatly to safe patient flow in dental practices. In addition, the ability for detection and differentiation of influenza and COVID-19, which have similar symptoms, will give insights for effective triage and treatment management.

Conclusions

Oral health care must continue to offer safety, despite the COVID-19 pandemic or future pandemics. Mitigation of SARS-CoV-2 transmission requires the application of engineering and administrative controls and the use of personal protective equipment. Although the promise of vaccines may reduce levels of the virus within communities, risks will remain until this virus falls into the annual viral background, and surveillance strategies at the community level remain important. Testing for SARS-CoV-2 at the POC offers another control mechanism contributing to and enhancing the real and perceived safety of care in the dental office setting. Several options for POC testing within the dental office are emerging (with additional responsibilities) and can provide additional guidance to reduce disease transmission in the dental office.