Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Suppression of a defective alanyl-tRNA synthetase in Escherichia coli: a compensatory mutation to high alanine affinity.

Literature DB >> 340903

Suppression of a defective alanyl-tRNA synthetase in Escherichia coli: a compensatory mutation to high alanine affinity.

Abstract

Among temperature resistant revertants of a temperature sensitive E. Coli alanyl-tRNA synthetase mutant a strain was found which contains an alanyl-tRNA synthetase with an additional mutation in the structural gene of the enzyme. This mutant enzyme has a 9 or 38 fold decreased Km value for alanine compared to that of the thermolabile parental enzyme or to wild-type enzyme, respectively. The alaS gene maps just counterclockwise from recA on the E. coli map (94% cotransduction frequency). It appears that the enzyme's increased affinity for alanine is the mechanism of suppressing the temperature sensitive character of the cell. In addition, some cold-sensitive temperature resistant revertants were found, where the cold-sensitive character mapped near strA. Presumably they are due to changes in ribosomal proteins as characterized by Ruffler et al. (1974).

Entities: Chemical Gene Species

Mesh：

Substances：

Year: 1977 PMID： 340903 DOI： 10.1007/bf00283495

Source DB: PubMed Journal: Mol Gen Genet ISSN： 0026-8925

19 in total

1. Sedimentation rate as a measure of molecular weight of DNA.

Authors: E BURGI; A D HERSHEY
Journal: Biophys J Date: 1963-07 Impact factor: 4.033

2. Biochemical and immunological characterization of threonyl-tRNA synthetase of two borrelidin-resistant mutants of Escherichia coli K12.

Authors: W Paetz; G Nass
Journal: Eur J Biochem Date: 1973-06

3. Temperature dependence of sex-factor maintenance in Escherichia coli K-12.

Authors: J Stadler; E A Adelberg
Journal: J Bacteriol Date: 1972-01 Impact factor: 3.490

4. Altered S5 and S20 ribosomal proteins in revertants of an alanyl-tRNA synthetase mutant of Escherichia coli.

Authors: H G Wittmann; G Stöffler
Journal: Mol Gen Genet Date: 1974

5. Mutation affecting the charging reaction of alanyl-tRNA synthetase from Escherichia coli K 10.

Authors: A Böck
Journal: Arch Mikrobiol Date: 1969-10

6. [On the properties of a modified alanyl-t-RNA synthetase in a strain of escherichia coli with thermosensible growth].

Authors: M Lazar; M Yaniv; F Gros
Journal: C R Acad Hebd Seances Acad Sci D Date: 1968-01-29

7. Isolation and partial characterization of temperature-sensitive Escherichia coli mutants with altered leucyl- and seryl-transfer ribonucleic acid synthetases.

Authors: B Low; F Gates; T Goldstein; D Söll
Journal: J Bacteriol Date: 1971-11 Impact factor: 3.490

8. Substrate specificity of a mutant alanyl-transfer ribonucleic acid synthetase of Escherichia coli.

Authors: P Buckel; W Lubitz; A Böck
Journal: J Bacteriol Date: 1971-12 Impact factor: 3.490

9. Suppression of temperature-sensitive aminoacyl-tRNA synthetase mutations by ribosomal mutations: a possible mechanism.

Authors: P Buckel; W Piepersberg; A Böck
Journal: Mol Gen Genet Date: 1976-11-24

10. Rapid mapping of conditional and auxotrophic mutations in Escherichia coli K-12.

Authors: B Low
Journal: J Bacteriol Date: 1973-02 Impact factor: 3.490

10 in total

1. Regulation of the biosynthesis of aminoacyl-transfer ribonucleic acid synthetases and of transfer ribonucleic acid in Escherichia coli. V. Mutants with increased levels of valyl-transfer ribonucleic acid synthetase.

Authors: M Baer; K B Low; D Söll
Journal: J Bacteriol Date: 1979-07 Impact factor: 3.490

2. Regulation of the biosynthesis of aminoacyl-tRNA synthetases and of tRNA in Escherichia coli. IV. Mutants with increased levels of leucyl- or seryl-tRNA synthetase.

Authors: G Theall; K B Low; D Söll
Journal: Mol Gen Genet Date: 1979-01-31

10. A DNA fragment with an alpha-phosphorothioate nucleotide at one end is asymmetrically blocked from digestion by exonuclease III and can be replicated in vivo.

Authors: S D Putney; S J Benkovic; P R Schimmel
Journal: Proc Natl Acad Sci U S A Date: 1981-12 Impact factor: 11.205

10 in total

Suppression of a defective alanyl-tRNA synthetase in Escherichia coli: a compensatory mutation to high alanine affinity.

1. Sedimentation rate as a measure of molecular weight of DNA.

2. Biochemical and immunological characterization of threonyl-tRNA synthetase of two borrelidin-resistant mutants of Escherichia coli K12.

3. Temperature dependence of sex-factor maintenance in Escherichia coli K-12.

4. Altered S5 and S20 ribosomal proteins in revertants of an alanyl-tRNA synthetase mutant of Escherichia coli.

5. Mutation affecting the charging reaction of alanyl-tRNA synthetase from Escherichia coli K 10.

6. [On the properties of a modified alanyl-t-RNA synthetase in a strain of escherichia coli with thermosensible growth].

7. Isolation and partial characterization of temperature-sensitive Escherichia coli mutants with altered leucyl- and seryl-transfer ribonucleic acid synthetases.

8. Substrate specificity of a mutant alanyl-transfer ribonucleic acid synthetase of Escherichia coli.

9. Suppression of temperature-sensitive aminoacyl-tRNA synthetase mutations by ribosomal mutations: a possible mechanism.

10. Rapid mapping of conditional and auxotrophic mutations in Escherichia coli K-12.

1. Regulation of the biosynthesis of aminoacyl-transfer ribonucleic acid synthetases and of transfer ribonucleic acid in Escherichia coli. V. Mutants with increased levels of valyl-transfer ribonucleic acid synthetase.

2. Regulation of the biosynthesis of aminoacyl-tRNA synthetases and of tRNA in Escherichia coli. IV. Mutants with increased levels of leucyl- or seryl-tRNA synthetase.

3. Hyper-recombining recipient strains in bacterial conjugation.

4. Attenuation of the ilvB operon by amino acids reflecting substrates or products of the ilvB gene product.

5. Isolation and characterization of spontaneous srl-recA deletion mutants in Escherichia coli K-12.

Review 6. Linkage map of Escherichia coli K-12, edition 6.

7. A base substitution in the amino acid acceptor stem of tRNA(Lys) causes both misacylation and altered decoding.

8. Deletion of an essential gene in Escherichia coli by site-specific recombination with linear DNA fragments.

9. Suppression of growth and protein secretion defects in Escherichia coli secA mutants by decreasing protein synthesis.

10. A DNA fragment with an alpha-phosphorothioate nucleotide at one end is asymmetrically blocked from digestion by exonuclease III and can be replicated in vivo.