The prognostic power of 18F-FDG PET/CT extends to estimating systemic treatment response duration in metastatic castration-resistant prostate cancer (mCRPC) patients.

BACKGROUND: We aimed to test whether the prognostic value of 18 F-Fluorodeoxyglucose Positron Emission Tomography/Computed Tomography (FDG-PET/CT) in metastatic castration-resistant prostate cancer (mCRPC) extends to the estimation of systemic treatment response duration.
METHODS: mCRPC patients submitted to FDG-PET/CT in four Italian centers from 2005 to 2020 were retrospectively enrolled. Clinical and biochemical data at the time of imaging were collected, and SUV max of the hottest lesion, total metabolic tumor volume (MTV), and total lesion glycolysis (TLG) were calculated. The correlation between PET- and biochemical-derived parameters with Overall Survival (OS) was analysed. The prediction of treatment response duration was assessed in the subgroup submitted to FDG-PET/CT in the six months preceding Chemotherapy (namely Docetaxel or Cabazitaxel, 24 patients) or Androgen-Receptor Targeted Agents (ARTA, namely Abiraterone or Enzalutamide, 20 patients) administration.
RESULTS: We enrolled 114 mCRPC patients followed-up for a median interval lasting 15 months. While at univariate analysis, prostate-specific antigen (PSA), Alkaline Phosphatase (ALP), MTV, and TLG were associated with OS, at the multivariate Cox regression analysis, the sole MTV could independently predict OS (p < 0.0001). In the subgroup submitted to FDG-PET/CT before the systemic treatment initiation, PSA and TLG could also predict treatment response duration independently (p < 0.05). Of note, while PSA could not indicate the best treatment choice, lower TLG was associated with higher success rates for ARTA but had no impact on chemotherapy efficacy.
CONCLUSIONS: FDG-PET/CT's prognostic value extends to predicting treatment response duration in mCRPC, thus potentially guiding the systemic treatment selection.

Introduction

In the last years, the therapeutic strategy for advanced prostate cancer (PC) radically changed due to improved knowledge of PC basic biology and progression mechanisms. Indeed, new molecules have been registered for the metastatic castration-resistant phase of the disease (mCRPC), and several emerging compounds are currently in the path of their validation.

Since combinations of these drugs have not been successful [1, 2], the sequential strategy remains the dominant approach in the clinical setting. However, given that the treatment-related environmental stress may promote the selection of aggressive and cross-resistant cancer cell populations, it is reasonable that each line of treatment will affect the duration of response of the subsequent lines [3]. Group analyses showed that treatment sequencing optimization may favorably impact clinical outcomes in mCRPC patients [4-7]. Nevertheless, choosing the right time for the proper therapy is complicated at an individual patient level. No prospective sequencing or head-to-head comparison trials are available, as conducting these studies is challenging, making level 1 evidence lacking. Consequently, the patient’s or tumor’s characteristics or other clinical/biochemical parameters (such as the PSA value, the previous chemotherapy, the disease burden, or the presence of visceral metastases) derived from retrospective or phase 2 studies still represent the major determinants of treatment selection [8, 9]. In this scenario, there is an urgent need to identify biomarkers able to predict therapy effectiveness before its administration (thus potentially improving treatment selection on an individual basis).

Preclinical studies showed the occurrence of a metabolic glucose reprogramming in mCRPC patients developing resistance to androgen receptors (AR) targeted therapies [10, 11]. On the other hand, in the last years, several studies pointed out the potential prognostic value for 18 F‐Fluorodeoxyglucose Positron Emission Tomography/ Computed Tomography (FDG-PET/CT) [12-18] in prostate cancer patients, including the capability to predict time to hormonal failure in the castration-sensitive phase of the disease [17]. These findings suggest that tumor burden’s metabolic changes may potentially guide the treatment selection process in mCRPC.

On these bases, the present retrospective multicentric study aims to verify whether FDG-PET/CT’s predictive power extends to estimating systemic treatment response duration in mCRPC, thus potentially improving the treatment selection process.

Patients and methods

Study population

We performed a retrospective multicentric analysis of all consecutive mCRPC patients who underwent FDG-PET/CT from January 2005 to February 2020 in four Italian Institutes (IRCCS Ospedale Policlinico San Martino of Genoa, Spedali Civili of Brescia, AUSL-IRCCS of Reggio Emilia, and University of Messina). CRPC was defined as a serum testosterone level of <50 ng/dl following surgical or pharmaceutical castration. Recruited patients were submitted to FDG-PET/CT as indicated by the national guidelines [19]. Medical history, patient’s age, Gleason Score (GS), Prostate-specific antigen (PSA) at diagnosis, as well as PSA alkaline phosphatase (ALP), and lactate dehydrogenase (LDH) at the time of PET/CT, as well as the results of CT and bone scans performed near to FDG-PET/CT were collected.

According to the standard procedure of all centers, all patients signed a written informed consent form at the time of PET/CT, encompassing the use of anonymized data for research purposes. The local ethical committees approved the retrospective multicentric study.

Imaging procedures and images analyses

FDG-PET/CT was performed according to the European Association of Nuclear Medicine (EANM) Guidelines [20]. Due to the retrospective design, PET/CT exams were acquired on different scanners, as detailed in Supplementary Table 1.

Maximum standardized uptake value (SUVmax) of the hottest metastatic lesion was obtained from FDG-PET/CT images. A volume of interest was then drawn using an SUV-based automated contouring program with an isocounter threshold based on 40% of the SUVmax [21]. The sum of all metastatic lesions identified the total Metabolic Tumor Volume (MTV). In contrast, the sum of the products between volume and the corresponding SUVmean of each lesion determined the Total Lesion Glycolysis (TLG).

Statistical analysis

Descriptive analyses were conducted using percentages for binary variables and means/medians for continuous variables, reporting their dispersion values.

After the binarization of continuous variables, GS, serum variables (PSA, ALP, LDH), and FDG-PET/CT-derived parameters (SUVmax, MTV, TLG), were assessed for their correlation with Overall Survival (OS) as independent variables using a univariable and multivariable Cox regression model. The Youden index from the ROC curve for survival data was used to find the best cut-off value for all variables but GS and the number of bone scan lesions. GS at diagnosis was categorized into two classes for clinical interpretation, as previously described [22], while bone scan lesions were classified in < 6, 6–20, and > 20, as previously described [23]. All parameters with a p value < 0.10 at univariable analysis were selected for the multivariable analysis. OS was calculated from the time of FDG-PET/CT to death from any cause, censored at last follow-up for patients who were alive. Hazard-ratio (HR) for Cox regression models were reported together with a 95% confidence interval (CI) and p value. A p value of <0.05 was considered statistically significant. Survival curves were also generated using a Kaplan–Meier (KM) approach.

We thus focused on the subgroup of patients submitted to FDG-PET/CT in the six months preceding the start of systemic treatment (either Chemotherapy or Androgen Receptor-Targeted Agents, ARTA). The same Cox regression model was performed considering the Progression-Free Survival (PFS) as the final endpoint. PFS was calculated from FDG-PET/CT to the date of first disease progression, relapse, death, or the last follow-up date.

A Linear Regression analysis was performed using the Pearson correlation coefficient to explore further the association between the analyzed parameters and treatment response duration.

Analyses were conducted with IBM‐SPSS release 23 (IBM, Armonk, USA).

Results

Patients’ and treatment characteristics

114 mCRPC patients were retrospectively enrolled. Clinical, tumor, and treatment characteristics are summarized in Table 1.

Table 1

Clinical characteristics of the study population (n = 114).

Clinical characteristics	n (%)
Median age, years (range)	73.62 (51.6–88.7)
Gleason score at diagnosis
≤7	56 (49.9%)
≥8	58 (50.1%)
Prostatectomy
Yes	40 (35.1%)
No	72 (63.2%)
Missing data	2 (1.7%)
Lymphadenectomy
Yes	33 (28.9%)
No	75 (65.8%)
Missing data	6 (5.3%)
Radical radiotherapy
Yes	11 (9.6%)
No	101 (88.6%)
Missing data	2 (1.8%)
Metastatic disease at diagnosis
Yes	62 (54.3%)
No	51 (44%)
Missing data	1 (1.7%)
Median PSA at diagnosis, ng/mL (range)	23 (1–6471)
Median ALP at diagnosis, U/L (range)	150.5 (33–613)
Median LDH at diagnosis, U/L (range)	234.5 (17–497)
Median interval between diagnosis and PET/CT, years (range)	5.6 (0.19–22.1)
Lines of treatment for CRPC at the time of FDG PET/CT
1	22 (19.3%)
2	31 (27.2%)
≥2	58 (50.9%)
Missing data	3 (2.6%)
Prior chemotherapy
Yes	64 (56.1%)
Docetaxel	46 (40.3%)
Cabazitaxel	18 (15.8%)
No	47 (41.2%)
Missing data	3 (2.6%)
Site of Metastases at the time of FDG PET/CT
Exclusive lymph node metastases	3 (2.6%)
Bone and lymph node metastases	95 (83.4%)
Visceral metastases	16 (14%)
N° bone metastases at the time of FDG PET/CT
<6	18 (15.7%)
6–20	40 (35.1%)
>20	32 (28.1%)
Missing data	24 (21.1%)
Median PSA at the time of FDG PET/CT, ng/mL (range)	64 (0.02–6471)
Median ALP at the time of FDG PET/CT, U/L (range)	100 (30–1266)
Median LDH at the time of FDG PET/CT, U/L (range)	271.2 (23–2349)

PSA prostate-specific antigen, ALP alkaline phosphatase, LDH lactate dehydrogenase, CRPC castration-resistant prostate cancer.

All patients had a histological diagnosis of PC with a median GS of 8 (range 5–10) with a GS ≥ 8 in 50.1% of patients; 54.3% of patients had metastatic disease at diagnosis. At the time of FDG-PET/CT, the median age was 81.6 years (range 52.7-88.7 years). CT revealed the occurrence of lymph node-only metastases in 2.6% of patients, while lymph node and bone metastases were present in 83.4% of patients. Visceral metastases were detected in 14% of patients. Most of the enrolled patients underwent FDG imaging after at least two systemic treatment lines for CRPC (50.8%), including chemotherapy (56.1%).

Overall survival analysis

All patients included in the study were assessable for overall survival analysis and were followed-up for a median of 15 months (range: 0.8–42 months). The median OS (mOS) was 12.7 months, with 6 months and 12 months-OS of 52% (Standard Error: 0.05) and 36% (Standard Error: 0.06), respectively. Supplementary Fig. 1 shows the Kaplan–Meier survival function of the study cohort. Results from univariable and multivariable Cox regression analyses are reported in Table 2.

Table 2

Clinical characteristics and FDG-derived parameters in the prediction of OS in the whole cohort (n = 114).

	Univariate		Multivariate
	HR (95% CI)	p value	HR (95% CI)	p value
Patients’ characteristics
Gleason score
≤7	1.00 (ref)	–
≥8	1.07 (0.66–1.74)	0.77
Metastatic disease at diagnosis
No	1.00 (ref)
Yes	1.31 (0.81–2.13)	0.26
PSA at diagnosis
≤22.5 ng/mL	1.00 (ref)	–
>22.5 ng/mL	1.470 (0.805–2.685)	0.21
ALP at diagnosis
≤67.5 IU/L	1.00 (ref)	–
>67.5 IU/L	2.448 (0.317–18.89)	0.39
LDH at diagnosis
≤223.5 IU/L	1.00 (ref)	–
>223.5 IU/L	0.608 (0.190–1.945)	0.402
Lines of treatment for CRPC at the time of PET/CT
1	1.00 (ref)	–
≥2	1.37 (0.79–2.39)	0.25
Prior chemotherapy
No	1.00 (ref)	–
Yes	1.55 (0.76–3.15)	0.22
Site of Metastases at the time of PET/CT
Exclusive lymph node	1.00 (ref)	–
Bone and lymph node	1.79 (0.24–13.02)	0.56
Visceral	2.12 (0.26–16.73)	0.47
No of bone metastases
<6	1.00 (ref)	–
6–20	1.95 (0.83–4.58)	0.12
≥20	3.13 (1.39-7.03)	0.006
PSA at PET/CT
≤58.3 ng/mL	1.00 (ref)	–
>58.3 ng/mL	3.996 (2.201–7.257)	0.0001	2.161 (1.014–4.606)	0.046
ALP at PET/CT
≤45.5 IU/L	1.00 (ref)	–
>45.5 IU/L	1.666 (0.662–4.196)	0.278
LDH at PET/CT
≤214.5 IU/L	1.00 (ref)	–
>214.5 IU/L	0.830 (0.247–2.787)	0.763
FDG-PET parameters
SUV max (1-unit)
≤6.9	1.00 (ref)	–
>6.9	1.221 (0.747–1.997)	0.426
MTV (1-unit)
≤325.97 cm3	1.00 (ref)	–
>325.97 cm3	2.288 (1.404–3.728)	0.001	2.647 (1.270–5.518)	0.009
TLG (1-unit)
≤844.86	1.00 (ref)	–
>844.86	2.208 (1.345–3.626)	0.002

PSA prostate-specific antigen, ALP alkaline phosphatase, LDH lactate dehydrogenase, CRPC castration-resistant prostate cancer, SUV standardized uptake value, MTV metabolic tumor volume, TLG total lesion glycolysis.

High PSA and ALP serum levels as well as the presence of ≥20 bone metastases at the time of PET/CT were associated with lower OS. On the other hand, lower MTV and TLG correlated with an increased OS. Obtained cut-off values from the ROC curve analysis for OS for these variables are reported in Supplementary Table 2. Kaplan–Meier curves for these parameters after the binarization of continuous variables are reported in Supplementary Fig. 2. MTV remained also independently associated with OS at the multivariate analysis (p < 0.0001).

Systemic treatment response duration

From the initial group of 114 mCRPC, we identified 44 patients submitted to FDG-PET/CT in the six months preceding systemic treatment initiation with either Chemotherapy (Docetaxel or Cabazitaxel, n = 24) or ARTA (Abiraterone or Enzalutamide, n = 20). The selection process details are reported in the Supplementary Fig. 3. No significant differences were observed between the obtained subgroups regarding clinical, tumor, and imaging characteristics (Supplementary Table 3).

These patients were clinically followed-up for a median interval of 18 months (range 0.8–42). At the survival analysis, the mOS of the entire group was 16.9 months, with 6 months and 12 months-OS of 68% (Standard Error: 0.08) and 46% (Standard Error: 0.1), respectively. The subgroup of patients undergoing ARTA showed an mOS of 19.1 months, while mOS was 14.4 months in patients undergoing Chemotherapy. However, this difference was not significant at the Log rank test (Supplementary Fig. 4A). The median PFS (mPFS) was 7.2 months, with 6 months and 12 months-PFS of 21% (Standard Error: 0.07) and 7% (Standard Error: 0.05), respectively. Again, mPFS was not significantly different between the ARTA and Chemotherapy subgroups, resulting 9.2 and 6.1 months, respectively (Supplementary Fig. 4B).

At the univariate analysis, PSA at the time of PET/CT, MTV, and TLG significantly predicted the systemic treatment response duration (PFS). Among them, TLG remained independently associated with PFS at the multivariate analysis (Table 3).

Table 3

Clinical characteristics and FDG-derived parameters in PFS prediction in the subgroup with pre-treatment PET (n = 44).

	Univariate		Multivariate
	HR (95% CI)	p value	HR (95% CI)	p value
Patients’ characteristics
Gleason score
≤7	1.00 (ref)	–
≥8	1.09 (0.54–2.16)	0.80
Metastatic disease at diagnosis
No	1.00 (ref)
Yes	1.36 (0.40–4.53)	0.61
PSA at diagnosis
≤18.1 ng/mL	1.00 (ref)	–
>18.1 ng/mL	0.82 (0.38–1.79)	0.629
ALP at diagnosis
≤150 IU/L	1.00 (ref)	–
>150 IU/L	0.26 (0.05–1.36)	0.113
LDH at diagnosis
≤262 IU/L	1.00 (ref)	–
>262 IU/L	0.45 (0.11–1.82)	0.267
Lines of treatment for CRPC at the time of PET/CT
1	1.00 (ref)	–
≥2	1.72 (0.61–4.84)	0.30
Prior chemotherapy
No	1.00 (ref)	–
Yes	1.70 (0.83–3.47)	0.14
Site of Metastases at the time of PET/CT
Exclusive lymph node	1.00 (ref)	–
Bone and lymph node	2836 (0.00–15000)	0.98
Visceral	5056 (0.00–26770)	0.21
No of bone metastases
<6	1.00 (ref)	–
6–20	1.88 (0.65–5.40)	0.23
≥20	2.44 (0.84–7.04)	0.09
PSA at PET/CT
≤35.5 ng/mL	1.00 (ref)	–
>35.5 ng/mL	2.32 (1.02–5.27)	0.043
ALP at PET/CT
≤100 IU/L	1.00 (ref)	–
>100 IU/L	0.92 (0.43–1.94)	0.827
LDH at PET/CT
≤236.5 IU/L	1.00 (ref)	–
>236.5 IU/L	1.06 (0.48–2.31)	0.878
FDG–PET parameters
SUV max (1-unit)
≤5.5	1.00 (ref)	–
>5.5	1.05 (0.50–2.23)	0.882
MTV (1-unit)
≤53 cm3	1.00 (ref)	–
>53 cm3	2.59 (1.13–5.90)	0.023
TLG (1-unit)
≤1820	1.00 (ref)	–
>1820	3.59 (1.60–8.03)	0.002	3.90 (1.67–9.12)	0.002

PSA prostate-specific antigen, ALP alkaline phosphatase, LDH lactate dehydrogenase, CRPC castration-resistant prostate cancer, SUV standardized uptake value, MTV metabolic tumor volume, TLG total lesion glycolysis.

Obtained cut-off values from the ROC curve for PFS for these variables are reported in Supplementary Table 4. When PSA was binarized, ARTA and Chemotherapy performed equally in the two obtained subgroups (Supplementary Fig. 5A). However, patients belonging to opposite TLG categories showed different susceptibilities to systemic treatments. Indeed, patients with lower TLG levels showed increased PFS when treated with ARTA than Chemotherapy, while these treatments performed equally in the presence of high TLG values (Supplementary Fig. 5B). This finding was also confirmed by the significant correlation between TLG and the duration of response to therapy, which was observed only in patients treated with ARTA (Fig. 1A). In other words, while PSA at the time of PET/CT could not predict the best treatment choice, the occurrence of lower TLG was able to predict higher success rates for ARTA but had no impact on chemotherapy efficacy (Fig. 1B, C). Two emblematic cases in which opposed TLG predicted divergent response duration to the same systemic treatment are reported in Fig. 2.

Fig. 1

TLG in the prediction of duration of response to systemic therapy.

A The linear correlation analyses between TLG and treatment response duration (months) in patients submitted to FDG PET/CT in the six months preceding the administration of systemic treatment with either chemotherapy or ARTA. B, C The Kaplan–Meier curves for PFS in patients with higher (green) and lower (blue) TLG levels at baseline before the administration of chemotherapy or ARTA, respectively.

Fig. 2

Emblematic cases of divergent response duration to the same systemic treatment in the presence of opposite TLG.

In these two patients, FDG PET/CT showed a remarkable mismatch in the extent of the metabolically active metastatic burden (TLG = 59, and TLG = 5602.4 in Panels A and B, respectively), which corresponded to divergent response duration to the same systemic treatment (20 and 4 months after the first dose of Enzalutamide, respectively). On the left side of each panel Maximum intensity projection (MIP) PET images are represented, while on the right side, the axial section of CT, PET and PET/CT images of the hottest metastatic lesions (also indicated by the orange line on the MIP images) are represented from top to bottom, respectively.

Discussion

The present study supports the role of FDG-PET/CT as a prognostic biomarker in hormone-refractory PC and demonstrates its capability to predict therapy response duration before the systemic treatment administration.

Several clinical features have been used to stratify OS in mCRPC, distinguishing between high and low-risk patients [24]. Meirelles [25] and Jadvar [13, 26] firstly reported that FDG-PET/CT could predict OS in these patients, even after adjusting for other prognostic clinical confounders. The present study confirmed these findings in a larger cohort of patients as the FDG-avid metastatic burden independently predicted OS even after adjusting for PSA at the time of PET/CT.

In the clinical setting, the FDG predictive power has been employed in several ways in mCRPC. In the post-treatment phase, it has been proposed to assess therapy response using PET Response Criteria in Solid Tumors (PERCIST) in patients treated with systemic chemotherapy [27] or Radium-223 [15]. The present data extend this tool’s prognostic value to the pre-treatment setting, showing that FDG-PET/CT may also predict therapy response duration when used before the systemic treatment administration. Indeed, in the presence of a lower extent of FDG-avid disease, ARTA seems to outperform chemotherapy while performing similarly in case of higher levels of metabolically active disease. Of note, even if predicting OS, PSA value at the time of PET/CT could not improve treatment selection.

The likeliest explanation for these findings is that lower FDG uptake reflects the prevalence of well-differentiated AR‐dependent disease (potentially susceptible to ARTA). In contrast, the FDG-avid disease burden mirrors the metabolic glucose reprogramming characterizing the dedifferentiated (eventually low PSA-releasing) mCRPC and the consequent low-response rate to AR-targeted therapies. This interpretation agrees with the previous observation that glucose transporter 1 (GLUT-1) is overexpressed in AR-independent cells [28], representing a signature of PC aggressiveness [29]. Further, Fox et al. previously analyzed 133 mCRPC patients through a dual tracer PET/CT approach (FDG and 18F-Fluorodihydrotestosterone), identifying the existence of at least four different phenotypes of metastatic lesions based on a dichotomous classification of imaging findings [30]. Using histopathology as a reference, the authors observed that when FDG-avid, mCRPC lesions are less differentiated and low expressing AR [30]. Moreover, the higher was the preponderance of the FDG + /AR- disease, the poorer was the clinical outcome [30]. Similar findings have been more recently reproduced using a dual tracer approach with FDG and Prostate-Specific Membrane Antigen (PSMA) in patients undergoing 177Lu-PSMA radioligand therapy [12, 31–34].

The present study provides new elements to support these previous findings. From the methodological point of view, it proposes quantifying the disease burden rather than merely defining the presence or absence of FDG-avidity. The prognostic value of high (versus low) burden disease is already established in PC. Previous studies with bone scan and PSMA PET/CT showed that the volumetric measurement of disease provides invaluable prognostic information that could result in a better treatment individualization [35, 36]. Furthermore, both the CHAARTED and STAMPEDE clinical trials [37, 38] introduced its use as a parameter to consider in the PC’s therapeutic management. However, an agreed consensus definition of the high-burden disease currently lacks. Jadvar et al. estimated it by the sum of SUVmax of all lesions [13, 18]. SUV, the most diffuse semiquantitative parameter, may be considered a surrogate of metabolic activity, as a high value of SUV usually reflects the tumor’s aggressiveness and poor prognosis. However, it intrinsically presents some limitations, including the strict dependency on blood glucose level, the eventual radiotracer’s extravasation, the body weight, the potential residual activity in the syringe, the uptake time, the radiotracer decay, the partial volume effect, the scanner features, the protocols of acquisition and elaboration [39, 40]. For these reasons, in addition to the usefulness of SUVmax, several studies indicated that MTV and TLG could provide superior prognostic information in various cancer types [41, 42]. We quantified the disease burden using these two parameters on these bases and according to a few previous studies [15, 16, 34, 43]. This approach allowed us to confirm that MTV is a robust predictor of long-term survival in mCRPC. This is in line with previous studies showing that the higher the tumor burden volume the shorter is long-term survival (regardless of treatment choices) [35, 36]. On the other hand, TLG overcame MTV in predicting systemic treatment response duration. TLG can be considered as a compromise between morphological and metabolic features, including the tumor size and the metabolic activity at the same time. The independent predictive value of TLG suggests that not only the extent (displayed by MTV, which is one of the TLG determinants) but also the intensity of the metastatic burden’s FDG uptake concur to define the systemic therapy’s response. As FDG uptake intensity reflects the degree of metabolic glucose reprogramming, TLG might provide pathophysiological insights into the PC biology in vivo, potentially better guiding the treatment choice (once integrated with tumor volume) compared to the volumetric estimation alone.

On the clinical ground, this finding has practical implications for PC’s drug development in the era of Precision Oncology [44, 45]. Indeed, cancer heterogeneity represents a significant challenge for this approach. Previous studies highlighted that circulating tumor cells represent a promising biomarker for predicting inadequate response to ARTA, potentially improving treatment individualization [46]. However, this approach is costly and not less available. Conversely, FDG-PET/CT may represent a widely available biomarker. Whether confirmed by further studies, FDG imaging may identify subgroups of patients most likely to benefit from a specific therapy, thus optimizing treatment sequencing. On the other hand, it may open new opportunities for targeting PC glucose metabolism in addition to or instead of AR-target therapies [11].

The present study has some limitations. First, due to its multicentric nature, we must assume that the PET scanners were not cross calibrated to ensure that the same SUV was measured when the same lesion was assessed on two different PET scanners. More importantly, the retrospective design did not allow us to prevent any potential patient selection bias. Indeed, the decision to order the FDG-PET/CT scan was made by the treating oncologist and was based on medical and non‐medical considerations, including the unavailability of other more specific PET radiotracers for prostate cancer (such as choline or PSMA). On these bases, we cannot exclude that, in some cases, mCRPC patients referred to FDG-PET/CT might have been considered by the treating physician at higher risk. This might have reduced the generalizability of our findings to most prostate cancer patients. The same consideration applies to the retrospective selection of mCRPC patients submitted to FDG-PET/CT in the six months preceding systemic treatment administration, which identified a subgroup of patients with remarkably high PSA levels compared to the initial cohort. Despite these concerns, to the best of our knowledge, the present study represents the first to determine the interplay between FDG avidity and systemic treatment response duration in mCRPC. However, the current results should be considered proof-of-concept findings to be used as the baseline to design future larger and prospective studies. Indeed, a 2-arm randomized design using cross-calibrated PET scanners is needed to more robustly demonstrate whether individual patient groups defined through FDG imaging may predict distinct treatment response patterns. As a further limitation, due to the low sample size, the survival analysis was not stratified according to each ARTA or Chemotherapy agent type, considering their common mechanisms of AR signaling inhibition or disruption of microtubule function, respectively. However, results may have differed regarding their distinct mechanisms of action. More importantly, given that Cabazitaxel is administered only in previously Docetaxel-treated patients, the same category included chemotherapy-naïve and already Docetaxel-treated patients. Despite the presence of a similar percentage of chemotherapy-naïve mCRPC in the ARTA subgroup might have reduced the impact of this methodological bias, this point still needs to be addressed in the next future. Similarly, we recognize that there has been a change in the treatment landscape for mCRPC in the last years, most notably with the introduction of novel antiandrogens. Larger patient numbers, longer follow-up, and more detailed analyses of treatment-related subgroups (including patients who have received docetaxel in the castrate-sensitive phase of the disease) will be required to corroborate our preliminary observation.

Conclusions

FDG-PET/CT provides relevant prognostic insights in hormone-refractory prostate cancer. PET-based parameters such as TLG, incorporating the estimation of both tumor metabolic activity and the extent of disease burden, can also predict systemic treatment response duration. Whether prospectively confirmed, this molecular imaging-based approach may improve treatment individualization, opening a new window on the clinical and biological mCRPC heterogeneity’s characterization.

Supplementary Materials

Supplementary Figure 1

Supplementary Figure 2

Supplementary Figure 3

Supplementary Figure 4

Supplementary Figure 5