Literature DB >> 3399506

Continuous, on-line DNA sequencing using oligodeoxynucleotide primers with multiple fluorophores.

J A Brumbaugh1, L R Middendorf, D L Grone, J L Ruth.   

Abstract

A method for sequencing DNA by using a difluoresceinated primer and laser excitation is described. Dideoxy protocols have been determined that provide sequences for 600 bases starting with base 1 with less than 1% error in a single load. Electrophoresis is at 20 W and the bands are detected 24 cm from the bottom of the loading well with a scanning fluorescence detector. Bands are imaged on a TV screen in two dimensions. The sequences can be read from the TV screen manually or semiautomatically by using a simple software program. The system allows more bases to be read with a lower error rate than any other reported automated sequencing method.

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Year:  1988        PMID： 3399506      PMCID： PMC281809          DOI： 10.1073/pnas.85.15.5610

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  17 in total

1.  A system for rapid DNA sequencing with fluorescent chain-terminating dideoxynucleotides.

Authors:  J M Prober; G L Trainor; R J Dam; F W Hobbs; C W Robertson; R J Zagursky; A J Cocuzza; M A Jensen; K Baumeister
Journal:  Science       Date:  1987-10-16       Impact factor: 47.728

2.  A turning point in cancer research: sequencing the human genome.

Authors:  R Dulbecco
Journal:  Science       Date:  1986-03-07       Impact factor: 47.728

3.  New M13 vectors for cloning.

Authors:  J Messing
Journal:  Methods Enzymol       Date:  1983       Impact factor: 1.600

4.  System for DNA sequencing with resolution of up to 600 base pairs.

Authors:  W Ansorge; R Barker
Journal:  J Biochem Biophys Methods       Date:  1984-03

5.  Improvements of DNA sequencing gels.

Authors:  H Garoff; W Ansorge
Journal:  Anal Biochem       Date:  1981-08       Impact factor: 3.365

6.  The synthesis of oligonucleotides containing an aliphatic amino group at the 5' terminus: synthesis of fluorescent DNA primers for use in DNA sequence analysis.

Authors:  L M Smith; S Fung; M W Hunkapiller; T J Hunkapiller; L E Hood
Journal:  Nucleic Acids Res       Date:  1985-04-11       Impact factor: 16.971

7.  DNA sequencing with chain-terminating inhibitors.

Authors:  F Sanger; S Nicklen; A R Coulson
Journal:  Proc Natl Acad Sci U S A       Date:  1977-12       Impact factor: 11.205

8.  Improvement of the dideoxy chain termination method of DNA sequencing by use of deoxy-7-deazaguanosine triphosphate in place of dGTP.

Authors:  S Mizusawa; S Nishimura; F Seela
Journal:  Nucleic Acids Res       Date:  1986-02-11       Impact factor: 16.971

9.  The plasmid cloning vector pBR325 contains a 482 base-pair-long inverted duplication.

Authors:  P Prentki; F Karch; S Iida; J Meyer
Journal:  Gene       Date:  1981-09       Impact factor: 3.688

10.  DNA sequencing with direct blotting electrophoresis.

Authors:  S Beck; F M Pohl
Journal:  EMBO J       Date:  1984-12-01       Impact factor: 11.598
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  29 in total

1.  Molecular cloning and sequencing of a cDNA for an auxin-repressed mRNA: correlation between fruit growth and repression of the auxin-regulated gene.

Authors:  A S Reddy; B W Poovaiah
Journal:  Plant Mol Biol       Date:  1990-02       Impact factor: 4.076

2.  DNA sequencing of four bases using three lanes.

Authors:  M Nelson; J L Van Etten; R Grabherr
Journal:  Nucleic Acids Res       Date:  1992-03-25       Impact factor: 16.971

3.  Characterization of a Euglena gene encoding a polyprotein precursor to the light-harvesting chlorophyll a/b-binding protein of photosystem II.

Authors:  U S Muchhal; S D Schwartzbach
Journal:  Plant Mol Biol       Date:  1992-01       Impact factor: 4.076

4.  Multiwavelength fluorescence detection for DNA sequencing using capillary electrophoresis.

Authors:  A E Karger; J M Harris; R F Gesteland
Journal:  Nucleic Acids Res       Date:  1991-09-25       Impact factor: 16.971

5.  Characterization of a maize cDNA that complements an enolase-deficient mutant of Escherichia coli.

Authors:  S K Lal; S Johnson; T Conway; P M Kelley
Journal:  Plant Mol Biol       Date:  1991-05       Impact factor: 4.076

6.  Imaging of DNA sequences with chemiluminescence.

Authors:  R Tizard; R L Cate; K L Ramachandran; M Wysk; J C Voyta; O J Murphy; I Bronstein
Journal:  Proc Natl Acad Sci U S A       Date:  1990-06       Impact factor: 11.205

7.  Capillary gel electrophoresis for rapid, high resolution DNA sequencing.

Authors:  H Swerdlow; R Gesteland
Journal:  Nucleic Acids Res       Date:  1990-03-25       Impact factor: 16.971

8.  Molecular analysis of the ADH1-Cm allele of maize.

Authors:  J C Osterman; E S Dennis
Journal:  Plant Mol Biol       Date:  1989-08       Impact factor: 4.076

9.  Maize pyruvate decarboxylase mRNA is induced anaerobically.

Authors:  P M Kelley
Journal:  Plant Mol Biol       Date:  1989-08       Impact factor: 4.076

10.  Oligonucleotides, part 5+: synthesis and fluorescence studies of DNA oligomers d(AT)5 containing adenines covalently linked at C-8 with dansyl fluorophore.

Authors:  D Singh; V Kumar; K N Ganesh
Journal:  Nucleic Acids Res       Date:  1990-06-11       Impact factor: 16.971
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