Literature DB >> 3951988

Improvement of the dideoxy chain termination method of DNA sequencing by use of deoxy-7-deazaguanosine triphosphate in place of dGTP.

S Mizusawa, S Nishimura, F Seela.   

Abstract

The dideoxy chain termination method using deoxy-7-deazaguanosine triphosphate (dc7GTP) in place of dGTP was found to be very useful. Sequencing of a part of the human N-myc gene having 85% GC content is impossible by the original method using dGTP, because of compression of bands. However, the nucleotide sequence of this part was unambiguously determined by analysis of both strands by the modified method. Use of dc7GTP is concluded to improve the dideoxy chain termination method for DNA sequencing.

Entities:  

Mesh：

Substances：

Year:  1986        PMID： 3951988      PMCID： PMC339506          DOI： 10.1093/nar/14.3.1319

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  5 in total

1.  Sequence diversity among related genes for recognition of specific targets in DNA molecules.

Authors:  J A Gough; N E Murray
Journal:  J Mol Biol       Date:  1983-05-05       Impact factor: 5.469

2.  Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors.

Authors:  C Yanisch-Perron; J Vieira; J Messing
Journal:  Gene       Date:  1985       Impact factor: 3.688

3.  Human N-myc gene contributes to neoplastic transformation of mammalian cells in culture.

Authors:  M Schwab; H E Varmus; J M Bishop
Journal:  Nature       Date:  1985 Jul 11-17       Impact factor: 49.962

4.  Poly(7-deazaguanylic acid), the homopolynucleotide of the parent nucleoside of queuosine.

Authors:  F Seela; Q H Tran-Thi; D Franzen
Journal:  Biochemistry       Date:  1982-08-31       Impact factor: 3.162

5.  DNA sequencing with chain-terminating inhibitors.

Authors:  F Sanger; S Nicklen; A R Coulson
Journal:  Proc Natl Acad Sci U S A       Date:  1977-12       Impact factor: 11.205
  5 in total
  235 in total

1.  Genetic and molecular analysis of cglB, a gene essential for single-cell gliding in Myxococcus xanthus.

Authors:  A M Rodriguez; A M Spormann
Journal:  J Bacteriol       Date:  1999-07       Impact factor: 3.490

2.  Unlocking hidden genomic sequence.

Authors:  Jonathan M Keith; Duncan A E Cochran; Gita H Lala; Peter Adams; Darryn Bryant; Keith R Mitchelson
Journal:  Nucleic Acids Res       Date:  2004-02-18       Impact factor: 16.971

3.  Characterization of the promoter region of the src family gene lyn and its trans activation by human T-cell leukemia virus type I-encoded p40tax.

Authors:  F Uchiumi; K Semba; Y Yamanashi; J Fujisawa; M Yoshida; K Inoue; K Toyoshima; T Yamamoto
Journal:  Mol Cell Biol       Date:  1992-09       Impact factor: 4.272

4.  Classification and characterization of the rice alpha-amylase multigene family.

Authors:  N Huang; T D Sutliff; J C Litts; R L Rodriguez
Journal:  Plant Mol Biol       Date:  1990-05       Impact factor: 4.076

5.  Cloning, characterization, and physical location of the rplY gene which encodes ribosomal protein L25 in Escherichia coli K12.

Authors:  Y Uemura; S Isono; K Isono
Journal:  Mol Gen Genet       Date:  1991-04

6.  Subcloning, nucleotide sequence, and expression of trkG, a gene that encodes an integral membrane protein involved in potassium uptake via the Trk system of Escherichia coli.

Authors:  A Schlösser; S Kluttig; A Hamann; E P Bakker
Journal:  J Bacteriol       Date:  1991-05       Impact factor: 3.490

7.  Suppression of the Escherichia coli rpoH opal mutation by ribosomes lacking S15 protein.

Authors:  R Yano; T Yura
Journal:  J Bacteriol       Date:  1989-03       Impact factor: 3.490

8.  virG, a plasmid-coded virulence gene of Shigella flexneri: identification of the virG protein and determination of the complete coding sequence.

Authors:  M C Lett; C Sasakawa; N Okada; T Sakai; S Makino; M Yamada; K Komatsu; M Yoshikawa
Journal:  J Bacteriol       Date:  1989-01       Impact factor: 3.490

9.  Nucleotide sequence and expression of a Streptomyces griseosporeus proteinaceous alpha-amylase inhibitor (HaimII) gene.

Authors:  H Nagaso; S Saito; H Saito; H Takahashi
Journal:  J Bacteriol       Date:  1988-10       Impact factor: 3.490

10.  Identification of a gene linked to Rhizobium meliloti ntrA whose product is homologous to a family to ATP-binding proteins.

Authors:  L M Albright; C W Ronson; B T Nixon; F M Ausubel
Journal:  J Bacteriol       Date:  1989-04       Impact factor: 3.490
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.