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Literature DB >> 2577507

Molecular analysis of the ADH1-Cm allele of maize.

Abstract

The Adh1-Cm allele and each gene in the Adh1-FCm duplication have been cloned and restriction-mapped. Of the Cm allele 6 kb was sequenced. A single amino acid substitution of aspartate for tyrosine at residue 52 accounts for the altered enzymatic properties of the Cm protein. Comparison of the nucleotide sequence to that of Adh1-1F and Adh1-1S shows structural and restriction site polymorphisms in the 3' flanking DNA. Cm lacks the insertion sequence present in 1F and 1S and contains a complex sequence composed of two direct repeats and an inverted repeat. The two genes of the duplication allele have similar restriction maps to Cm and each other.

Entities: Gene Mutation Species

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Year: 1989 PMID： 2577507 DOI： 10.1007/bf00016138

Source DB: PubMed Journal: Plant Mol Biol ISSN： 0167-4412 Impact factor: 4.076

20 in total

Molecular analysis of the ADH1-Cm allele of maize.

1. Alcohol Dehydrogenase Polymorphism in Maize-simple and Compound Loci.

2. Allelic variation at the level of intragenic recombination.

3. Continuous, on-line DNA sequencing using oligodeoxynucleotide primers with multiple fluorophores.

4. Primary structure and subunit stoichiometry of F1-ATPase from bovine mitochondria.

5. Exceptionally High Levels of Restriction Site Polymorphism in DNA near the Maize Adh1 Gene.

6. The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers.

7. Lambda replacement vectors carrying polylinker sequences.

8. DNA sequences required for anaerobic expression of the maize alcohol dehydrogenase 1 gene.

9. In vivo detection of regulatory factor binding sites in the 5' flanking region of maize Adh1.

10. Correlation of exons with structural domains in alcohol dehydrogenase.

1. Copy number lability and evolutionary dynamics of the Adh gene family in diploid and tetraploid cotton (Gossypium).

2. The highly recombinogenic bz locus lies in an unusually gene-rich region of the maize genome.

3. Tourist: a large family of small inverted repeat elements frequently associated with maize genes.

4. New nucleotide sequence data on the EMBL File Server.

5. Evolution of alcohol dehydrogenase genes in the palm and grass families.

6. Evolution of the rice Xa21 disease resistance gene family.

7. Comparison of droplet digital PCR with quantitative real-time PCR for determination of zygosity in transgenic maize.

8. Mobile inverted-repeat elements of the Tourist family are associated with the genes of many cereal grasses.

9. Molecular evolution of alcohol dehydrogenase 1 in members of the grass family.

10. Evolution of Ac and Dsl elements in select grasses (Poaceae).