Literature DB >> 3380683

Parameters of field inversion gel electrophoresis for the analysis of pox virus genomes.

C J Bostock1.   

Abstract

The effects of variation in the lengths of forward and reverse pulses, voltage gradient, gel concentration and gel temperature on the mobility of DNA molecules in agarose gels during field inversion gel electrophoresis (FIGE) have been determined. A curve, which best fits the empirical data, is presented and allows the choice of pulse conditions and voltage gradient most suitable for the resolution of molecules of chosen size. The use of FIGE in the analysis and direct mapping of large virus genomes is illustrated using vaccinia virus DNA.

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Year:  1988        PMID： 3380683      PMCID： PMC336627          DOI： 10.1093/nar/16.10.4239

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  12 in total

1.  Conservation and variation in Orthopoxvirus genome structure.

Authors:  M Mackett; L C Archard
Journal:  J Gen Virol       Date:  1979-12       Impact factor: 3.891

2.  A model for the separation of large DNA molecules by crossed field gel electrophoresis.

Authors:  E M Southern; R Anand; W R Brown; D S Fletcher
Journal:  Nucleic Acids Res       Date:  1987-08-11       Impact factor: 16.971

3.  Electrophoretic separations of large DNA molecules by periodic inversion of the electric field.

Authors:  G F Carle; M Frank; M V Olson
Journal:  Science       Date:  1986-04-04       Impact factor: 47.728

4.  Mapping the limits of the human pseudoautosomal region and a candidate sequence for the male-determining gene.

Authors:  C A Pritchard; P J Goodfellow; P N Goodfellow
Journal:  Nature       Date:  1987 Jul 16-22       Impact factor: 49.962

5.  Two major DNA variants present in serially propagated stocks of the WR strain of vaccinia virus.

Authors:  D Panicali; S W Davis; S R Mercer; E Paoletti
Journal:  J Virol       Date:  1981-03       Impact factor: 5.103

6.  Separation of chromosomal DNA molecules from yeast by orthogonal-field-alternation gel electrophoresis.

Authors:  G F Carle; M V Olson
Journal:  Nucleic Acids Res       Date:  1984-07-25       Impact factor: 16.971

7.  Megabase-scale mapping of the HLA gene complex by pulsed field gel electrophoresis.

Authors:  S K Lawrance; C L Smith; R Srivastava; C R Cantor; S M Weissman
Journal:  Science       Date:  1987-03-13       Impact factor: 47.728

8.  Separation of yeast chromosome-sized DNAs by pulsed field gradient gel electrophoresis.

Authors:  D C Schwartz; C R Cantor
Journal:  Cell       Date:  1984-05       Impact factor: 41.582

9.  An electrophoretic karyotype for Schizosaccharomyces pombe by pulsed field gel electrophoresis.

Authors:  C L Smith; T Matsumoto; O Niwa; S Klco; J B Fan; M Yanagida; C R Cantor
Journal:  Nucleic Acids Res       Date:  1987-06-11       Impact factor: 16.971

10.  A bovine papilloma virus vector with a dominant resistance marker replicates extrachromosomally in mouse and E. coli cells.

Authors:  P D Matthias; H U Bernard; A Scott; G Brady; T Hashimoto-Gotoh; G Schütz
Journal:  EMBO J       Date:  1983       Impact factor: 11.598
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  11 in total

1.  Field inversion gel electrophoresis with different pulse time ramps.

Authors:  C Heller; F M Pohl
Journal:  Nucleic Acids Res       Date:  1990-11-11       Impact factor: 16.971

2.  A systematic study of field inversion gel electrophoresis.

Authors:  C Heller; F M Pohl
Journal:  Nucleic Acids Res       Date:  1989-08-11       Impact factor: 16.971

3.  Field inversion gel electrophoresis in denaturing polyacrylamide gels.

Authors:  C Heller; S Beck
Journal:  Nucleic Acids Res       Date:  1992-05-25       Impact factor: 16.971

4.  The impact of two-dimensional pulsed-field gel electrophoresis techniques for the consistent and complete mapping of bacterial genomes: refined physical map of Pseudomonas aeruginosa PAO.

Authors:  U Römling; B Tümmler
Journal:  Nucleic Acids Res       Date:  1991-06-25       Impact factor: 16.971

5.  The basis of high resolution separation of small DNAs by asymmetric-voltage field inversion electrophoresis and its application to DNA sequencing gels.

Authors:  B W Birren; M I Simon; E Lai
Journal:  Nucleic Acids Res       Date:  1990-03-25       Impact factor: 16.971

6.  Identification of temperature-sensitive mutants of vaccinia virus that are defective in conversion of concatemeric replicative intermediates to the mature linear DNA genome.

Authors:  A M DeLange
Journal:  J Virol       Date:  1989-06       Impact factor: 5.103

7.  Physical mapping and field inversion gel electrophoresis of Amsacta moorei entomopoxvirus DNA.

Authors:  R L Hall; W F Hink
Journal:  Arch Virol       Date:  1990       Impact factor: 2.574

8.  Introduction of transposon Tn916 DNA into Haemophilus influenzae and Haemophilus parainfluenzae.

Authors:  L Kauc; S H Goodgal
Journal:  J Bacteriol       Date:  1989-12       Impact factor: 3.490

9.  Construction of chimeric vaccinia viruses by molecular cloning and packaging.

Authors:  F Scheiflinger; F Dorner; F G Falkner
Journal:  Proc Natl Acad Sci U S A       Date:  1992-11-01       Impact factor: 11.205

10.  Increase in local protein concentration by field-inversion gel electrophoresis.

Authors:  Henghang Tsai; Teck Yew Low; Steve Freeby; Aran Paulus; Kalpana Ramnarayanan; Chung-Pui Paul Cheng; Hon-Chiu Eastwood Leung
Journal:  Proteome Sci       Date:  2007-09-26       Impact factor: 2.480
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