Literature DB >> 2528121

A systematic study of field inversion gel electrophoresis.

C Heller1, F M Pohl.   

Abstract

The mobilities of oligomers of phage lambda DNA and of yeast chromosomes in agarose gels during field inversion gel electrophoresis (FIGE) were measured at different pulse times and electric fields. Also the ratios between forward and backward pulse times and/or field gradients were varied. The problem of 'band inversion' during FIGE, leading to an ambiguity in the mobility of large DNA fragments, was solved by using two dimensional gel electrophoresis with different parameters in the first and second dimension. The results are compared with those obtained with other pulsed electrophoresis systems and with a theoretical model.

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Year:  1989        PMID： 2528121      PMCID： PMC318255          DOI： 10.1093/nar/17.15.5989

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  37 in total

1.  Pulsed field gel electrophoresis techniques for separating 1- to 50-kilobase DNA fragments.

Authors:  B W Birren; E Lai; L Hood; M I Simon
Journal:  Anal Biochem       Date:  1989-03       Impact factor: 3.365

2.  An equation for DNA electrophoretic mobility in agarose gels.

Authors:  C E Willis; D G Willis; G P Holmquist
Journal:  Appl Theor Electrophor       Date:  1988

3.  High-resolution separation and accurate size determination in pulsed-field gel electrophoresis of DNA. 2. Effect of pulse time and electric field strength and implications for models of the separation process.

Authors:  M K Mathew; C L Smith; C R Cantor
Journal:  Biochemistry       Date:  1988-12-27       Impact factor: 3.162

4.  Low-field transient electric birefringence of DNA in agarose gels.

Authors:  B Chu; R Xu; Z Wang
Journal:  Biopolymers       Date:  1988-12       Impact factor: 2.505

5.  Conformational dynamics of individual DNA molecules during gel electrophoresis.

Authors:  D C Schwartz; M Koval
Journal:  Nature       Date:  1989-04-06       Impact factor: 49.962

6.  Transient orientation of linear DNA molecules during pulsed-field gel electrophoresis.

Authors:  G Holzwarth; C B McKee; S Steiger; G Crater
Journal:  Nucleic Acids Res       Date:  1987-12-10       Impact factor: 16.971

7.  Electrophoretic separations of large DNA molecules by periodic inversion of the electric field.

Authors:  G F Carle; M Frank; M V Olson
Journal:  Science       Date:  1986-04-04       Impact factor: 47.728

8.  Observation of individual DNA molecules undergoing gel electrophoresis.

Authors:  S B Smith; P K Aldridge; J B Callis
Journal:  Science       Date:  1989-01-13       Impact factor: 47.728

9.  Separation of chromosomal DNA molecules from yeast by orthogonal-field-alternation gel electrophoresis.

Authors:  G F Carle; M V Olson
Journal:  Nucleic Acids Res       Date:  1984-07-25       Impact factor: 16.971

10.  Separation of yeast chromosome-sized DNAs by pulsed field gradient gel electrophoresis.

Authors:  D C Schwartz; C R Cantor
Journal:  Cell       Date:  1984-05       Impact factor: 41.582
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  13 in total

1.  Field inversion gel electrophoresis with different pulse time ramps.

Authors:  C Heller; F M Pohl
Journal:  Nucleic Acids Res       Date:  1990-11-11       Impact factor: 16.971

2.  Ultrafast, efficient separations of large-sized dsDNA in a blended polymer matrix by microfluidic chip electrophoresis: a design of experiments approach.

Authors:  Mingyun Sun; Jennifer S Lin; Annelise E Barron
Journal:  Electrophoresis       Date:  2011-10-18       Impact factor: 3.535

3.  Field inversion gel electrophoresis in denaturing polyacrylamide gels.

Authors:  C Heller; S Beck
Journal:  Nucleic Acids Res       Date:  1992-05-25       Impact factor: 16.971

4.  Phage diversity in a methanogenic digester.

Authors:  M-O Park; H Ikenaga; K Watanabe
Journal:  Microb Ecol       Date:  2006-12-22       Impact factor: 4.552

5.  Fluctuations in the velocity of individual DNA Molecules during agarose gel electrophoresis.

Authors:  T D Howard; G Holzwarth
Journal:  Biophys J       Date:  1992-12       Impact factor: 4.033

6.  Reptation theories of electrophoresis.

Authors:  J L Viovy
Journal:  Mol Biotechnol       Date:  1996-08       Impact factor: 2.695

7.  The basis of high resolution separation of small DNAs by asymmetric-voltage field inversion electrophoresis and its application to DNA sequencing gels.

Authors:  B W Birren; M I Simon; E Lai
Journal:  Nucleic Acids Res       Date:  1990-03-25       Impact factor: 16.971

8.  Methods for pulsed-field gel electrophoresis.

Authors:  J T Den Dunnen; G J van Ommen
Journal:  Appl Biochem Biotechnol       Date:  1993 Jan-Feb       Impact factor: 2.926

9.  Physical map of the genome of Acholeplasma oculi ISM1499 and construction of a Tn4001 derivative for macrorestriction chromosomal mapping.

Authors:  E Tigges; F C Minion
Journal:  J Bacteriol       Date:  1994-02       Impact factor: 3.490

10.  Investigating DNA migration in pulsed fields using a miniaturized FIGE system.

Authors:  Xiaojia Chen; Victor M Ugaz
Journal:  Electrophoresis       Date:  2008-12       Impact factor: 3.535
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