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Literature DB >> 2243776

Field inversion gel electrophoresis with different pulse time ramps.

Abstract

The influence of different pulse time ramps on the separation of yeast chromosomes with field inversion gel electrophoresis (FIGE) was investigated by the means of two dimensional gel electrophoresis. The problem of band inversion, which makes it difficult to distinguish DNA molecules of different size, has been solved by using double randomized pulse times. A major disadvantage of the field inversion technique is thereby overcome, making this system comparable to other pulsed field techniques.

Entities: Species

Mesh：

Substances：
DNA, Fungal

Year: 1990 PMID： 2243776 PMCID： PMC332495 DOI： 10.1093/nar/18.21.6299

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

33 in total

1. Molecular detrapping and band narrowing with high frequency modulation of pulsed field electrophoresis.

Authors: C Turmel; E Brassard; G W Slater; J Noolandi
Journal: Nucleic Acids Res Date: 1990-02-11 Impact factor: 16.971

2. DNA trapping electrophoresis.

Authors: L Ulanovsky; G Drouin; W Gilbert
Journal: Nature Date: 1990-01-11 Impact factor: 49.962

3. Control of pulsed field gel electrophoresis at short switching intervals by a microcomputer.

Authors: H Hennekes; S Kühn
Journal: Anal Biochem Date: 1989-11-15 Impact factor: 3.365

4. A novel instrument for separating large DNA molecules with pulsed homogeneous electric fields.

Authors: S M Clark; E Lai; B W Birren; L Hood
Journal: Science Date: 1988-09-02 Impact factor: 47.728

5. Pulsed field gel electrophoresis techniques for separating 1- to 50-kilobase DNA fragments.

Authors: B W Birren; E Lai; L Hood; M I Simon
Journal: Anal Biochem Date: 1989-03 Impact factor: 3.365

6. High-resolution separation and accurate size determination in pulsed-field gel electrophoresis of DNA. 2. Effect of pulse time and electric field strength and implications for models of the separation process.

Authors: M K Mathew; C L Smith; C R Cantor
Journal: Biochemistry Date: 1988-12-27 Impact factor: 3.162

7. Electrophoretic separations of large DNA molecules by periodic inversion of the electric field.

Authors: G F Carle; M Frank; M V Olson
Journal: Science Date: 1986-04-04 Impact factor: 47.728

8. Separation of chromosomal DNA molecules from yeast by orthogonal-field-alternation gel electrophoresis.

Authors: G F Carle; M V Olson
Journal: Nucleic Acids Res Date: 1984-07-25 Impact factor: 16.971

9. Separation of yeast chromosome-sized DNAs by pulsed field gradient gel electrophoresis.

Authors: D C Schwartz; C R Cantor
Journal: Cell Date: 1984-05 Impact factor: 41.582

10. Direct detection of more than 50% of the Duchenne muscular dystrophy mutations by field inversion gels.

Authors: J T den Dunnen; E Bakker; E G Breteler; P L Pearson; G J van Ommen
Journal: Nature Date: 1987 Oct 15-21 Impact factor: 49.962

5 in total

1. Cloning and physical mapping of the HLA class I region spanning the HLA-E-to-HLA-F interval by using yeast artificial chromosomes.

Authors: D E Geraghty; J Pei; B Lipsky; J A Hansen; P Taillon-Miller; S K Bronson; D D Chaplin
Journal: Proc Natl Acad Sci U S A Date: 1992-04-01 Impact factor: 11.205