Literature DB >> 3295780

An electrophoretic karyotype for Schizosaccharomyces pombe by pulsed field gel electrophoresis.

C L Smith, T Matsumoto, O Niwa, S Klco, J B Fan, M Yanagida, C R Cantor.   

Abstract

The three chromosomal DNAs of S. pombe have been fractionated by pulsed field gel electrophoresis. The resulting molecular karyotype will greatly speed gene mapping in this organism, and it indicates that the separation range of the technique extends to DNA molecules as large as 9,000,000 base pairs.

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Year:  1987        PMID： 3295780      PMCID： PMC340875          DOI： 10.1093/nar/15.11.4481

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  17 in total

1.  Chromosome-sized DNA molecules of Plasmodium falciparum.

Authors:  L H Van der Ploeg; M Smits; T Ponnudurai; A Vermeulen; J H Meuwissen; G Langsley
Journal:  Science       Date:  1985-08-16       Impact factor: 47.728

2.  Electrophoretic separations of large DNA molecules by periodic inversion of the electric field.

Authors:  G F Carle; M Frank; M V Olson
Journal:  Science       Date:  1986-04-04       Impact factor: 47.728

3.  Karyotype analysis of Leishmania species and its use in classification and clinical diagnosis.

Authors:  S H Giannini; M Schittini; J S Keithly; P W Warburton; C R Cantor; L H Van der Ploeg
Journal:  Science       Date:  1986-05-09       Impact factor: 47.728

4.  The Number of Chromosomes in SCHIZOSACCHAROMYCES POMBE: Light Microscopy of Stained Preparations.

Authors:  C F Robinow
Journal:  Genetics       Date:  1977-11       Impact factor: 4.562

5.  New techniques for purifying large DNAs and studying their properties and packaging.

Authors:  D C Schwartz; W Saffran; J Welsh; R Haas; M Goldenberg; C R Cantor
Journal:  Cold Spring Harb Symp Quant Biol       Date:  1983

6.  Separation of chromosomal DNA molecules from yeast by orthogonal-field-alternation gel electrophoresis.

Authors:  G F Carle; M V Olson
Journal:  Nucleic Acids Res       Date:  1984-07-25       Impact factor: 16.971

7.  Separation of yeast chromosome-sized DNAs by pulsed field gradient gel electrophoresis.

Authors:  D C Schwartz; C R Cantor
Journal:  Cell       Date:  1984-05       Impact factor: 41.582

8.  The NDA3 gene of fission yeast encodes beta-tubulin: a cold-sensitive nda3 mutation reversibly blocks spindle formation and chromosome movement in mitosis.

Authors:  Y Hiraoka; T Toda; M Yanagida
Journal:  Cell       Date:  1984-12       Impact factor: 41.582

9.  Chromosome walking shows a highly homologous repetitive sequence present in all the centromere regions of fission yeast.

Authors:  Y Nakaseko; Y Adachi; S Funahashi; O Niwa; M Yanagida
Journal:  EMBO J       Date:  1986-05       Impact factor: 11.598

10.  Sequential alterations in the nuclear chromatin region during mitosis of the fission yeast Schizosaccharomyces pombe: video fluorescence microscopy of synchronously growing wild-type and cold-sensitive cdc mutants by using a DNA-binding fluorescent probe.

Authors:  T Toda; M Yamamoto; M Yanagida
Journal:  J Cell Sci       Date:  1981-12       Impact factor: 5.285
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  66 in total

1.  Detection of DNA domains in Drosophila, human, and plant chromosomes possessing mainly 50- to 150-kilobase stretches of DNA.

Authors:  N A Tchurikov; N A Ponomarenko
Journal:  Proc Natl Acad Sci U S A       Date:  1992-08-01       Impact factor: 11.205

2.  Electrophoretic karyotype for Dictyostelium discoideum.

Authors:  E C Cox; C D Vocke; S Walter; K Y Gregg; E S Bain
Journal:  Proc Natl Acad Sci U S A       Date:  1990-11       Impact factor: 11.205

3.  Giardia lamblia: haploid genome size determined by pulsed field gel electrophoresis is less than 12 Mb.

Authors:  J B Fan; S H Korman; C R Cantor; C L Smith
Journal:  Nucleic Acids Res       Date:  1991-04-25       Impact factor: 16.971

4.  Pulsed-field electrophoresis of megabase-sized DNA.

Authors:  K Gunderson; G Chu
Journal:  Mol Cell Biol       Date:  1991-06       Impact factor: 4.272

5.  Secondary pulsed field gel electrophoresis: a new method for faster separation of larger DNA molecules.

Authors:  T Y Zhang; C L Smith; C R Cantor
Journal:  Nucleic Acids Res       Date:  1991-03-25       Impact factor: 16.971

6.  A systematic study of field inversion gel electrophoresis.

Authors:  C Heller; F M Pohl
Journal:  Nucleic Acids Res       Date:  1989-08-11       Impact factor: 16.971

7.  A eukaryotic genome of 660 kb: electrophoretic karyotype of nucleomorph and cell nucleus of the cryptomonad alga, Pyrenomonas salina.

Authors:  S Eschbach; C J Hofmann; U G Maier; P Sitte; P Hansmann
Journal:  Nucleic Acids Res       Date:  1991-04-25       Impact factor: 16.971

8.  Ultrafast, efficient separations of large-sized dsDNA in a blended polymer matrix by microfluidic chip electrophoresis: a design of experiments approach.

Authors:  Mingyun Sun; Jennifer S Lin; Annelise E Barron
Journal:  Electrophoresis       Date:  2011-10-18       Impact factor: 3.535

9.  Analysis of DNA restriction fragments greater than 5.7 Mb in size from the centromeric region of human chromosomes.

Authors:  P H Arn; X Li; C Smith; M Hsu; D C Schwartz; E W Jabs
Journal:  Mamm Genome       Date:  1991       Impact factor: 2.957

10.  Comparison of restriction enzyme analysis and pulsed-field gradient gel electrophoresis as typing systems for Candida albicans.

Authors:  J A Vazquez; A Beckley; J D Sobel; M J Zervos
Journal:  J Clin Microbiol       Date:  1991-05       Impact factor: 5.948
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