Literature DB >> 3366934

Evaluation of ELISA, RPLA, and Vero cell assays for detecting Clostridium perfringens enterotoxin in faecal specimens.

P R Berry1, J C Rodhouse, S Hughes, B A Bartholomew, R J Gilbert.   

Abstract

Three hundred and ninety two faecal specimens from 70 separate outbreaks of suspected Clostridium perfringens food poisoning were examined by enzyme linked immunosorbent assay (ELISA), reversed passive latex agglutination (RPLA), and Vero cell assays for the presence of enterotoxin. Although the most time consuming method, ELISA was the most specific and reproducible. RPLA was slightly more sensitive than ELISA, but it showed some non-specific reactions. The Vero cell assay was the least sensitive and least reproducible method, being affected by some non-specific cytotoxic and cytotonic reactions. Normal rabbit serum should be included in the Vero cell assay as a control for the neutralisation of cytotoxic effects.

Entities:  

Mesh：

Substances：

Year:  1988        PMID： 3366934      PMCID： PMC1141476          DOI： 10.1136/jcp.41.4.458

Source DB:  PubMed          Journal:  J Clin Pathol        ISSN： 0021-9746            Impact factor:   3.411


  14 in total

1.  Experimental Diarrhoea in human volunteers following oral administration of Clostridium perfringens enterotoxin.

Authors:  R Skjelkvåle; T Uemura
Journal:  J Appl Bacteriol       Date:  1977-10

2.  Characterization of membrane permeability alterations induced in Vero cells by Clostridium perfringens enterotoxin.

Authors:  B A McClane; J L McDonel
Journal:  Biochim Biophys Acta       Date:  1980-08-14

3.  Simplified procedure for tissue culture in routine detection of cytotoxins.

Authors:  A R Welch; S P Borriello; F E Barclay
Journal:  J Clin Pathol       Date:  1985-07       Impact factor: 3.411

4.  Effects of Ca2+ and other cations on the action of Clostridium perfringens enterotoxin.

Authors:  Y Horiguchi; T Uemura; S Kozaki; G Sakaguchi
Journal:  Biochim Biophys Acta       Date:  1986-10-31

5.  Protective effects of osmotic stabilizers on morphological and permeability alterations induced in Vero cells by Clostridium perfringens enterotoxin.

Authors:  B A McClane; J L McDonel
Journal:  Biochim Biophys Acta       Date:  1981-03-06

6.  Bovine serum eliminates rapid nonspecific toxic reactions during bioassay of stored fish for Clostridium botulinum toxin.

Authors:  M Solberg; L S Post; D Furgang; C Graham
Journal:  Appl Environ Microbiol       Date:  1985-03       Impact factor: 4.792

7.  Persistent high numbers of Clostridium perfringens in the intestines of Japanese aged adults.

Authors:  T Yamagishi; T Serikawa; R Morita; S Nakamura; S Nishida
Journal:  Jpn J Microbiol       Date:  1976-10

8.  Development and application of an enzyme linked immunosorbent assay for Clostridium perfringens type A enterotoxin.

Authors:  B A Bartholomew; M F Stringer; G N Watson; R J Gilbert
Journal:  J Clin Pathol       Date:  1985-02       Impact factor: 3.411

9.  Ileal loop fluid accumulation and production of diarrhea in rabbits by cell-free products of Clostridium perfringens.

Authors:  C L Duncan; D H Strong
Journal:  J Bacteriol       Date:  1969-10       Impact factor: 3.490

10.  Detection of Clostridium perfringens enterotoxin by tissue culture and double-gel diffusion methods.

Authors:  L G Giugliano; M F Stringer; B S Drasar
Journal:  J Med Microbiol       Date:  1983-05       Impact factor: 2.472
View more
  18 in total

1.  Evidence for antibiotic induced Clostridium perfringens diarrhoea.

Authors:  N Modi; M H Wilcox
Journal:  J Clin Pathol       Date:  2001-10       Impact factor: 3.411

2.  Recurrent diarrhea associated with enterotoxigenic Clostridium perfringens in 2 dogs.

Authors:  J S Weese; S J Greenwood; H R Staempfli
Journal:  Can Vet J       Date:  2001-04       Impact factor: 1.008

Review 3.  Toxigenic clostridia.

Authors:  C L Hatheway
Journal:  Clin Microbiol Rev       Date:  1990-01       Impact factor: 26.132

Review 4.  Clostridial enteric diseases of domestic animals.

Authors:  J G Songer
Journal:  Clin Microbiol Rev       Date:  1996-04       Impact factor: 26.132

Review 5.  Diagnostic Accuracy of Nucleic Acid Amplification-Based Assays for Clostridium perfringens-Associated Diseases: a Systematic Review and Meta-analysis.

Authors:  Ke Chen; Sarfraz Ahmed; Yun-Juan Sheng; Changfeng Sun; Cun-Liang Deng; Suvash Chandra Ojha
Journal:  J Clin Microbiol       Date:  2020-08-24       Impact factor: 5.948

6.  Molecular subtyping of Clostridium perfringens by pulsed-field gel electrophoresis to facilitate food-borne-disease outbreak investigations.

Authors:  S E Maslanka; J G Kerr; G Williams; J M Barbaree; L A Carson; J M Miller; B Swaminathan
Journal:  J Clin Microbiol       Date:  1999-07       Impact factor: 5.948

7.  Potential Therapeutic Effects of Mepacrine against Clostridium perfringens Enterotoxin in a Mouse Model of Enterotoxemia.

Authors:  Mauricio A Navarro; Archana Shrestha; John C Freedman; Juliann Beingesser; Bruce A McClane; Francisco A Uzal
Journal:  Infect Immun       Date:  2019-03-25       Impact factor: 3.441

8.  Vero cell assay for rapid detection of Clostridium perfringens enterotoxin.

Authors:  D E Mahony; E Gilliatt; S Dawson; E Stockdale; S H Lee
Journal:  Appl Environ Microbiol       Date:  1989-09       Impact factor: 4.792

9.  Clostridium perfringens toxin genotypes in the feces of healthy North Americans.

Authors:  Robert J Carman; Sameera Sayeed; Jihong Li; Christopher W Genheimer; Megan F Hiltonsmith; Tracy D Wilkins; Bruce A McClane
Journal:  Anaerobe       Date:  2008-02-07       Impact factor: 3.331

10.  Blinded application of microscopy, bacteriological culture, immunoassays and PCR to detect gastrointestinal pathogens from faecal samples of patients with community-acquired diarrhoea.

Authors:  C F L Amar; C East; E Maclure; J McLauchlin; C Jenkins; P Duncanson; D R A Wareing
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2004-06-16       Impact factor: 3.267
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.