Literature DB >> 6302258

Detection of Clostridium perfringens enterotoxin by tissue culture and double-gel diffusion methods.

L G Giugliano, M F Stringer, B S Drasar.   

Abstract

Culture filtrates of four enterotoxin-producing strains of Clostridium perfringens type A produced morphological changes in cells of 10 of 12 established mammalian cell lines tested, and particularly Vero (African green-monkey kidney) and MDCK (dog kidney) cells. Double-gel diffusion and Vero-cell tests detected enterotoxin in culture filtrates of, respectively, 29 (28%) and 56 (54%) of 103 strains of C. perfringens tested. The feasibility of cell-culture assays for the routine detection of C. perfringens enterotoxin is indicated.

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Year:  1983        PMID： 6302258     DOI： 10.1099/00222615-16-2-233

Source DB:  PubMed          Journal:  J Med Microbiol        ISSN： 0022-2615            Impact factor:   2.472


  3 in total

1.  Noncytotoxic Clostridium perfringens enterotoxin (CPE) variants localize CPE intestinal binding and demonstrate a relationship between CPE-induced cytotoxicity and enterotoxicity.

Authors:  James G Smedley; Juliann Saputo; Jacquelyn C Parker; Mariano E Fernandez-Miyakawa; Susan L Robertson; Bruce A McClane; Francisco A Uzal
Journal:  Infect Immun       Date:  2008-05-27       Impact factor: 3.441

2.  Evaluation of ELISA, RPLA, and Vero cell assays for detecting Clostridium perfringens enterotoxin in faecal specimens.

Authors:  P R Berry; J C Rodhouse; S Hughes; B A Bartholomew; R J Gilbert
Journal:  J Clin Pathol       Date:  1988-04       Impact factor: 3.411

3.  Vero cell assay for rapid detection of Clostridium perfringens enterotoxin.

Authors:  D E Mahony; E Gilliatt; S Dawson; E Stockdale; S H Lee
Journal:  Appl Environ Microbiol       Date:  1989-09       Impact factor: 4.792
  3 in total

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