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Literature DB >> 336638

Enzyme-linked immunosorbent assay for detection of Escherichia coli heat-labile enterotoxin.

R H Yolken, H B Greenberg, M H Merson, R B Sack, A Z Kapikian.

Abstract

The development of an enzyme-linked immunosorbent assay (ELISA) for the detection of heat-labile Escherichia coli enterotoxin is described. The assay, which is based on the immunological similarity between Vibrio cholerae toxin and heat-labile E. coli enterotoxin, is similar in design to a radioimmunoassay but utilizes enzyme-labeled rather than radioactive isotope-labeled reagents. The ELISA system is as sensitive as both radioimmunoassay and the y-1 adrenal cell assay for the detection of heat-labile E. coli enterotoxin but requires neither radioactive reagents nor tissue culture techniques. The ELISA is easy to perform and is adaptable for use in small laboratories.

Entities: Species

Mesh：

Substances：
Enterotoxins

Year: 1977 PMID： 336638 PMCID： PMC274794 DOI： 10.1128/jcm.6.5.439-444.1977

Source DB: PubMed Journal: J Clin Microbiol ISSN： 0095-1137 Impact factor: 5.948

17 in total

1. A study of the pathogenicity of strains of Bacterium coli from acute and chronic enteritis.

Authors: S N DE; K BHATTACHARYA; J K SARKAR
Journal: J Pathol Bacteriol Date: 1956-01

2. Epidemiologic assessment of the relevance of the so-called enteropathogenic serogroups of Escherichia coli in diarrhea.

Authors: E J Gangarosa; M H Merson
Journal: N Engl J Med Date: 1977-05-26 Impact factor: 91.245

3. "Enteropathogenic serotypes" of Escherichia coli which really are not.

Authors: J J Farmer; B R Davis; W B Cherry; D J Brenner; V R Dowell; A Balows
Journal: J Pediatr Date: 1977-06 Impact factor: 4.406

4. Solid-phase enzyme-immunoassay for detection of hepatitis B surface antigen.

Authors: G Wolters; L Kuijpers; J Kacaki; A Schuurs
Journal: J Clin Pathol Date: 1976-10 Impact factor: 3.411

5. Enzyme-linked immunosorbent assays for cholera serology.

Authors: J Holmgren; A M Svennerholm
Journal: Infect Immun Date: 1973-05 Impact factor: 3.441

6. Inhibition of immune hemolysis: serological assay for the heat-labile enterotoxin of Excherichia coli.

Authors: D J Evans; D G Evans
Journal: J Clin Microbiol Date: 1977-01 Impact factor: 5.948

7. Direct serological assay for the heat-labile enterotoxin of Escherichia coli, using passive immune hemolysis.

Authors: D J Evans; D G Evans
Journal: Infect Immun Date: 1977-05 Impact factor: 3.441

8. Microtiter solid-phase radioimmunoassay for detection of Escherichia coli heat-labile enterotoxin.

Authors: H B Greenberg; D A Sack; W Rodriguez; R B Sack; R G Wyatt; A R Kalica; R L Horswood; R M Chanock; A Z Kapikian
Journal: Infect Immun Date: 1977-09 Impact factor: 3.441

9. Cyclic adenosine monophosphate and alteration of Chinese hamster ovary cell morphology: a rapid, sensitive in vitro assay for the enterotoxins of Vibrio cholerae and Escherichia coli.

Authors: R L Guerrant; L L Brunton; T C Schnaitman; L I Rebhun; A G Gilman
Journal: Infect Immun Date: 1974-08 Impact factor: 3.441

10. Enzyme-linked immunosorbent assay (ELISA) for detection of human reovirus-like agent of infantile gastroenteritis.

Authors: R H Yolken; H W Kim; T Clem; R G Wyatt; A R Kalica; R M Chanock; A Z Kapikian
Journal: Lancet Date: 1977-08-06 Impact factor: 79.321

62 in total

1. Ultrasensitive enzymatic radioimmunoassay: application to detection of cholera toxin and rotavirus.

Authors: C C Harris; R H Yolken; H Krokan; I C Hsu
Journal: Proc Natl Acad Sci U S A Date: 1979-10 Impact factor: 11.205

2. Comparison of a latex agglutination assay and an enzyme-linked immunosorbent assay for detecting cholera toxin.

Authors: R J Almeida; F W Hickman-Brenner; E G Sowers; N D Puhr; J J Farmer; I K Wachsmuth
Journal: J Clin Microbiol Date: 1990-01 Impact factor: 5.948

3. Identification of enterotoxigenic Escherichia coli by colony hybridization with nonradioactive digoxigenin-labeled DNA probes.

Authors: L K Riley; C J Caffrey
Journal: J Clin Microbiol Date: 1990-06 Impact factor: 5.948

4. GM1 ganglioside enzyme-linked immunosorbent assay for detection of heat-labile enterotoxin produced by human and porcine Escherichia coli strains.

Authors: B Gustafsson; R Möllby
Journal: J Clin Microbiol Date: 1982-02 Impact factor: 5.948

5. Enzyme-linked immunosorbent assays for detection of thermostable direct hemolysin of Vibrio parahaemolyticus.

Authors: T Honda; M Yoh; U Kongmuang; T Miwatani
Journal: J Clin Microbiol Date: 1985-09 Impact factor: 5.948

6. Laboratory observations on Plesiomonas shigelloides strains isolated from children with diarrhea in Peru.

Authors: O Olsvik; K Wachsmuth; B Kay; K A Birkness; A Yi; B Sack
Journal: J Clin Microbiol Date: 1990-05 Impact factor: 5.948

7. Enzyme-linked immunosorbent assay for detection of Staphylococcus aureus alpha-toxin.

Authors: O P Surujballi; H B Fackrell
Journal: J Clin Microbiol Date: 1984-03 Impact factor: 5.948

8. Development and evaluation of enzyme-linked immunosorbent assays for detection of shiga-like toxin I and shiga-like toxin II.

Authors: F P Downes; J H Green; K Greene; N Strockbine; J G Wells; I K Wachsmuth
Journal: J Clin Microbiol Date: 1989-06 Impact factor: 5.948

9. Adaptation of the staphylococcal coagglutination technique for detection of heat-labile enterotoxin of Escherichia coli.

Authors: B M Brill; B L Wasilauskas; S H Richardson
Journal: J Clin Microbiol Date: 1979-01 Impact factor: 5.948

10. Enzyme-linked immunosorbent assay for measurement of serological response to respiratory syncytial virus infection.

Authors: L S Richardson; R H Yolken; R B Belshe; E Camargo; H W Kim; R M Chanock
Journal: Infect Immun Date: 1978-06 Impact factor: 3.441