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Literature DB >> 6371045

Enzyme-linked immunosorbent assay for detection of Staphylococcus aureus alpha-toxin.

Abstract

A sandwich enzyme-linked immunosorbent assay was developed for measuring Staphylococcus aureus alpha-toxin. This assay was 500 to 1,000 times more sensitive than the commonly used hemolytic titration assay and was less variable. The binding of alpha-toxin to the adsorbed antibody was most effective after an overnight incubation at 27 degrees C. The toxin was detectable even at a log2 17 dilution of an S. aureus culture supernatant.

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Year: 1984 PMID： 6371045 PMCID： PMC271072 DOI： 10.1128/jcm.19.3.394-398.1984

Source DB: PubMed Journal: J Clin Microbiol ISSN： 0095-1137 Impact factor: 5.948

29 in total

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Authors: M J O'Sullivan; J W Bridges; V Marks
Journal: Ann Clin Biochem Date: 1979-09 Impact factor: 2.057

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Authors: A B Dalen
Journal: Acta Pathol Microbiol Scand B Date: 1976-12

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Authors: M N Al-Bassam; M J O'Sullivan; E Gnemmi; J W Bridges; V Marks
Journal: Clin Chem Date: 1978-09 Impact factor: 8.327

4. Characteristics of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses.

Authors: M F Clark; A N Adams
Journal: J Gen Virol Date: 1977-03 Impact factor: 3.891

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Authors: J P Arbuthnott; J H Freer; A C McNiven
Journal: Contrib Microbiol Immunol Date: 1973

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Authors: G B Wisdom
Journal: Clin Chem Date: 1976-08 Impact factor: 8.327

7. Immunologic evidence that staphylococcal alpha toxin is oriented on membranes.

Authors: C Y Lo; H B Fackrell
Journal: Can J Microbiol Date: 1979-06 Impact factor: 2.419

8. Detection of circulating antigen during acute infections with Toxoplasma gondii by enzyme-linked immunosorbent assay.

Authors: F van Knapen; S O Panggabean
Journal: J Clin Microbiol Date: 1977-12 Impact factor: 5.948

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Authors: L Z Cooper; M A Madoff; L Weinstein
Journal: J Bacteriol Date: 1966-05 Impact factor: 3.490

10. Enzyme-linked immunosorbent assay for detection of Escherichia coli heat-labile enterotoxin.

Authors: R H Yolken; H B Greenberg; M H Merson; R B Sack; A Z Kapikian
Journal: J Clin Microbiol Date: 1977-11 Impact factor: 5.948

4 in total

1. Competitive, enzyme-linked immunosorbent assay for toxic shock syndrome toxin 1.

Authors: J Parsonnet; J T Mills; Z A Gillis; G B Pier
Journal: J Clin Microbiol Date: 1985-07 Impact factor: 5.948

2. Rapid screening for Staphylococcus aureus infection by measuring enterotoxin B.

Authors: K K Hosotsubo; H Hosotsubo; M K Nishijima; M Nishimura; N Taenaka; I Yoshiya
Journal: J Clin Microbiol Date: 1989-12 Impact factor: 5.948

3. Enzyme-linked immunosorbent assay for detection of leukocidin toxin from Staphylococcus aureus in bovine milk samples.

Authors: D A Loeffler; M T Creasy; N L Norcross; M J Paape
Journal: J Clin Microbiol Date: 1988-07 Impact factor: 5.948

4. In vitro selection of single-stranded DNA molecular recognition elements against S. aureus alpha toxin and sensitive detection in human serum.

Authors: Ka L Hong; Luisa Battistella; Alysia D Salva; Ryan M Williams; Letha J Sooter
Journal: Int J Mol Sci Date: 2015-01-27 Impact factor: 5.923

4 in total