Literature DB >> 69877

Enzyme-linked immunosorbent assay (ELISA) for detection of human reovirus-like agent of infantile gastroenteritis.

R H Yolken, H W Kim, T Clem, R G Wyatt, A R Kalica, R M Chanock, A Z Kapikian.   

Abstract

An enzyme-linked immunosorbent assay (ELISA) was developed for the detection of the human reovirus-like agent of infantile gastroenteritis in human stools. The results of the assay can be read either with a simple colorimeter or the naked eye. Investigations with 143 samples from children with gastroenteritis and 75 samples from children with other illnesses showed that the ELISA was as sensitive as electron microscopy or radioimmunoassay for detection of this agent. In addition, the ELISA was simple to perform and, when read visually, did not require sophisticated technical equipment. These advantages make it suitable for field work.

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Year:  1977        PMID: 69877     DOI: 10.1016/s0140-6736(77)90951-5

Source DB:  PubMed          Journal:  Lancet        ISSN: 0140-6736            Impact factor:   79.321


  113 in total

1.  Enzyme-linked fluorescence assay: Ultrasensitive solid-phase assay for detection of human rotavirus.

Authors:  R H Yolken; P J Stopa
Journal:  J Clin Microbiol       Date:  1979-09       Impact factor: 5.948

2.  Comparison of electron microscopy, enzyme-linked immunosorbent assay, solid-phase radioimmunoassay, and indirect immunofluorescence for detection of human rotavirus antigen in faeces.

Authors:  C J Birch; N I Lehmann; A J Hawker; J A Marshall; I D Gust
Journal:  J Clin Pathol       Date:  1979-07       Impact factor: 3.411

3.  Use of electron microscopy and an enzyme-linked immunosorbent assay for the detection of rotaviruses in neonatal calf diarrhea.

Authors:  P Payment; G Marsolais; M Trudel; M Fauvel; L Lamontagne; R Assaf; P Marois
Journal:  Can J Comp Med       Date:  1979-07

4.  Oral rehydration and maintenance of children with rotavirus and bacterial diarrhoeas.

Authors:  D R Nalin; M M Levine; L Mata; C de Céspedes; W Vargas; C Lizano; A R Loria; A Simhon; E Mohs
Journal:  Bull World Health Organ       Date:  1979       Impact factor: 9.408

5.  Ultrasensitive enzymatic radioimmunoassay: application to detection of cholera toxin and rotavirus.

Authors:  C C Harris; R H Yolken; H Krokan; I C Hsu
Journal:  Proc Natl Acad Sci U S A       Date:  1979-10       Impact factor: 11.205

Review 6.  Viruses in the stools.

Authors:  C R Madeley
Journal:  J Clin Pathol       Date:  1979-01       Impact factor: 3.411

7.  Measurement of rotavirus antibody by an enzyme-linked immunosorbent assay blocking assay.

Authors:  R H Yolken; R G Wyatt; B A Barbour; H W Kim; A Z Kapikian; R M Chanock
Journal:  J Clin Microbiol       Date:  1978-09       Impact factor: 5.948

8.  Double blind trial of loperamide for treating acute watery diarrhoea in expatriates in Bangladesh.

Authors:  F P van Loon; M L Bennish; P Speelman; C Butler
Journal:  Gut       Date:  1989-04       Impact factor: 23.059

9.  Detection of rotavirus in sewage.

Authors:  J Steinmann
Journal:  Appl Environ Microbiol       Date:  1981-04       Impact factor: 4.792

10.  Hemagglutinin-specific enzyme-linked immunosorbent assay for antibodies to influenza A and B viruses.

Authors:  B R Murphy; M A Phelan; D L Nelson; R Yarchoan; E L Tierney; D W Alling; R M Chanock
Journal:  J Clin Microbiol       Date:  1981-03       Impact factor: 5.948

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