| Literature DB >> 33589617 |
Jonathan Y Hsu1,2,3, Julian Grünewald2,3,4, Regan Szalay2,3, Justine Shih2,3, Andrew V Anzalone5,6,7, Kin Chung Lam2,3,4, Max W Shen5,6,7,8, Karl Petri2,3,4, David R Liu5,6,7, J Keith Joung9,10,11, Luca Pinello12,13,14.
Abstract
Prime editing (PE) is a versatile genome editing technology, but design of the required guide RNAs is more complex than for standard CRISPR-based nucleases or base editors. Here we describe PrimeDesign, a user-friendly, end-to-end web application and command-line tool for the design of PE experiments. PrimeDesign can be used for single and combination editing applications, as well as genome-wide and saturation mutagenesis screens. Using PrimeDesign, we construct PrimeVar, a comprehensive and searchable database that includes candidate prime editing guide RNA (pegRNA) and nicking sgRNA (ngRNA) combinations for installing or correcting >68,500 pathogenic human genetic variants from the ClinVar database. Finally, we use PrimeDesign to design pegRNAs/ngRNAs to install a variety of human pathogenic variants in human cells.Entities:
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Year: 2021 PMID： 33589617 PMCID： PMC7884779 DOI： 10.1038/s41467-021-21337-7
Source DB: PubMed Journal: Nat Commun ISSN： 2041-1723 Impact factor: 14.919