| Literature DB >> 33526904 |
Christoph Niemöller1,2,3,4, Julius Wehrle1,2,5, Julian Riba6, Rainer Claus1,2,7, Nathalie Renz1,2,7, Janika Rhein1,2, Sabine Bleul1,2, Juliane M Stosch1,2,4, Justus Duyster1,2,3, Christoph Plass8, Pavlo Lutsik8, Daniel B Lipka9, Michael Lübbert1,2,3, Heiko Becker10,11,12.
Abstract
Single-cell multi-omics are powerful means to study cell-to-cell heterogeneity. Here, we present a single-tube, bisulfite-free method for the simultaneous, genome-wide analysis of DNA methylation and genetic variants in single cells: epigenomics and genomics of single cells analyzed by restriction (epi-gSCAR). By applying this method, we obtained DNA methylation measurements of up to 506,063 CpGs and up to 1,244,188 single-nucleotide variants from single acute myeloid leukemia-derived cells. We demonstrate that epi-gSCAR generates accurate and reproducible measurements of DNA methylation and allows to differentiate between cell lines based on the DNA methylation and genetic profiles.Entities:
Year: 2021 PMID： 33526904 PMCID： PMC7851132 DOI： 10.1038/s42003-021-01661-w
Source DB: PubMed Journal: Commun Biol ISSN： 2399-3642