| Literature DB >> 25915121 |
Iain C Macaulay1, Wilfried Haerty2, Parveen Kumar3, Yang I Li2, Tim Xiaoming Hu2, Mabel J Teng4, Mubeen Goolam5, Nathalie Saurat6, Paul Coupland7, Lesley M Shirley7, Miriam Smith7, Niels Van der Aa3, Ruby Banerjee8, Peter D Ellis7, Michael A Quail7, Harold P Swerdlow7, Magdalena Zernicka-Goetz5, Frederick J Livesey6, Chris P Ponting9, Thierry Voet10.
Abstract
The simultaneous sequencing of a single cell's genome and transcriptome offers a powerful means to dissect genetic variation and its effect on gene expression. Here we describe G&T-seq, a method for separating and sequencing genomic DNA and full-length mRNA from single cells. By applying G&T-seq to over 220 single cells from mice and humans, we discovered cellular properties that could not be inferred from DNA or RNA sequencing alone.Entities:
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Year: 2015 PMID： 25915121 DOI： 10.1038/nmeth.3370
Source DB: PubMed Journal: Nat Methods ISSN： 1548-7091 Impact factor: 28.547