| Literature DB >> 10037822 |
M Matz1, D Shagin, E Bogdanova, O Britanova, S Lukyanov, L Diatchenko, A Chenchik.
Abstract
A new method for amplifying cDNA ends is described which requires only first-strand cDNA synthesis and a single PCR to generate a correct product with very low or no background. The method can be successfully applied to total RNA as well as poly A+ RNA. The same first-strand cDNA can be used to amplify flanking sequences of any cDNA species present in the sample.Mesh：
Substances：
Year: 1999 PMID： 10037822 PMCID： PMC148354 DOI： 10.1093/nar/27.6.1558
Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971