Literature DB >> 333399

Studies on gene control regions. VI. The 5- methyl of thymine, a lac repressor recognition site.

D V Goeddel, D G Yansura, M H Caruthers.   

Abstract

Three site specific deoxyuridine analogs of lac operator were tested for binding with wild type (SQ) and tight binding (QX86) lac repressors. Insertion of uracil for thymine at site 13 (our nomenclature) significantly reduced the dissociation half-life of QX86 repressor for lac operator DNA (21 vs 1.2 min). Two other sites (6 and 7) are affected to a much lesser extent.

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Year:  1977        PMID： 333399      PMCID： PMC342633          DOI： 10.1093/nar/4.9.3039

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  19 in total

1.  Chain length determination of small double- and single-stranded DNA molecules by polyacrylamide gel electrophoresis.

Authors:  T Maniatis; A Jeffrey; H van deSande
Journal:  Biochemistry       Date:  1975-08-26       Impact factor: 3.162

2.  Studies of gene control regions. III. Binding of synthetic and modified synthetic lac operator DNAs to lactose repressor.

Authors:  D G Yansura; D V Goeddel; D L Cribbs; M H Caruthers
Journal:  Nucleic Acids Res       Date:  1977-03       Impact factor: 16.971

3.  A new method for sequencing DNA.

Authors:  A M Maxam; W Gilbert
Journal:  Proc Natl Acad Sci U S A       Date:  1977-02       Impact factor: 11.205

4.  Minimal length of the lactose operator sequence for the specific recognition by the lactose repressor.

Authors:  C P Bahl; R Wu; J Stawinsky; S A Narang
Journal:  Proc Natl Acad Sci U S A       Date:  1977-03       Impact factor: 11.205

Review 5.  The role of DNA structure in genetic regulation.

Authors:  R D Wells; R W Blakesley; S C Hardies; G T Horn; J E Larson; E Selsing; J F Burd; H W Chan; J B Dodgson; K F Jensen; I F Nes; R M Wartell
Journal:  CRC Crit Rev Biochem       Date:  1977

6.  Studies on polynucleotides. 118. A further study of ribonucleotide incorporation into deoxyribonucleic acid chains by deoxyribonucleic acid polymerase I of Escherichia coli.

Authors:  J H Van de Sande; P C Loewen; H G Khorana
Journal:  J Biol Chem       Date:  1972-10-10       Impact factor: 5.157

7.  CIV. Total synthesis of the structural gene for an alanine transfer ribonucleic acid from yeast. Chemical synthesis of an icosadeoxynucleotide corresponding to the nucleotide sequence 21 to 40.

Authors:  H Weber; H G Khorana
Journal:  J Mol Biol       Date:  1972-12-28       Impact factor: 5.469

8.  Physical characterization and simultaneous purification of bacteriophage T4 induced polynucleotide kinase, polynucleotide ligase, and deoxyribonucleic acid polymerase.

Authors:  A Panet; J H van de Sande; P C Loewen; H G Khorana; A J Raae; J R Lillehaug; K Kleppe
Journal:  Biochemistry       Date:  1973-12-04       Impact factor: 3.162

9.  Studies on gene control regions. 2. Enzymatic joining of chemically synthesized lactose operator deoxyribonucleic acid segments.

Authors:  D G Yansura; D V Goeddel; M H Caruthers
Journal:  Biochemistry       Date:  1977-05-03       Impact factor: 3.162

10.  Tight-binding repressors of the lactose operon.

Authors:  J L Betz; J R Sadler
Journal:  J Mol Biol       Date:  1976-08-05       Impact factor: 5.469
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  9 in total

1.  How lac repressor recognizes lac operator.

Authors:  D V Goeddel; D G Yansura; M H Caruthers
Journal:  Proc Natl Acad Sci U S A       Date:  1978-08       Impact factor: 11.205

2.  Enzymatic synthesis of oligodeoxyribonucleotides of defined sequence. Polynucleotide phosphorylase catalysed synthesis using pyrimidine analog-containing deoxyribonucleoside 5'-diphosphates.

Authors:  E M Trip; M Smith
Journal:  Nucleic Acids Res       Date:  1978-05       Impact factor: 16.971

3.  Studies on gene control regions X. The effect of specific adenine-thymine transversions on the lac repressor-lac operator interaction.

Authors:  H S Sista; R T Loder; M H Caruthers
Journal:  Nucleic Acids Res       Date:  1979-06-11       Impact factor: 16.971

4.  Lac repressor - lac operator interaction. Circular dichroism study.

Authors:  F Culard; J C Maurizot
Journal:  Nucleic Acids Res       Date:  1981-10-10       Impact factor: 16.971

5.  The synthesis of 2-pyrimidinone nucleosides and their incorporation into oligodeoxynucleotides.

Authors:  B Gildea; L W McLaughlin
Journal:  Nucleic Acids Res       Date:  1989-03-25       Impact factor: 16.971

6.  On the influence of thymidine analogues on the activity of phage fd promoters in vitro.

Authors:  B Hofer; H Köster
Journal:  Nucleic Acids Res       Date:  1980-12-20       Impact factor: 16.971

7.  Studies on gene control regions XII. The functional significance of a lac operator constitutive mutation.

Authors:  E F Fisher; M H Caruthers
Journal:  Nucleic Acids Res       Date:  1979-09-25       Impact factor: 16.971

8.  A new approach to the synthesis of a protected 2-aminopurine derivative and its incorporation into oligodeoxynucleotides containing the Eco RI and Bam HI recognition sites.

Authors:  L W McLaughlin; T Leong; F Benseler; N Piel
Journal:  Nucleic Acids Res       Date:  1988-06-24       Impact factor: 16.971

9.  DNA chain length markers and the influence of base composition on electrophoretic mobility of oligodeoxyribonucleotides in polyacrylamide-gels.

Authors:  R Frank; H Köster
Journal:  Nucleic Acids Res       Date:  1979       Impact factor: 16.971
  9 in total

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