Literature DB >> 3320963

Molecular cloning and nucleotide sequence of the gene for Escherichia coli leucyl-tRNA synthetase.

M Härtlein1, D Madern.   

Abstract

The gene for Escherichia coli leucyl-tRNA synthetase leuS has been cloned by complementation of a leuS temperature sensitive mutant KL231 with an E.coli gene bank DNA. The resulting clones overexpress leucyl-tRNA synthetase (LeuRS) by a factor greater than 50. The DNA sequence of the complete coding regions was determined. The derived N-terminal protein sequence of LeuRS was confirmed by independent protein sequencing of the first 8 aminoacids. Sequence comparison of the LeuRS sequence with all aminoacyl-tRNA synthetase sequences available reveal a significant homology with the valyl-, isoleucyl- and methionyl-enzyme indicating that the genes of these enzymes could have derived from a common ancestor. Sequence comparison with the gene product of the yeast nuclear NAM2-1 suppressor allele curing mitochondrial RNA maturation deficiency reveals about 30% homology.

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Year:  1987        PMID： 3320963      PMCID： PMC339939          DOI： 10.1093/nar/15.24.10199

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  31 in total

1.  Is there a discriminator site in transfer RNA?

Authors:  D M Crothers; T Seno; G Söll
Journal:  Proc Natl Acad Sci U S A       Date:  1972-10       Impact factor: 11.205

2.  Content of elongation factor Tu in Escherichia coli.

Authors:  A V Furano
Journal:  Proc Natl Acad Sci U S A       Date:  1975-12       Impact factor: 11.205

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Authors:  M Sprinzl; F Cramer
Journal:  Proc Natl Acad Sci U S A       Date:  1975-08       Impact factor: 11.205

4.  Determinant of cistron specificity in bacterial ribosomes.

Authors:  J Shine; L Dalgarno
Journal:  Nature       Date:  1975-03-06       Impact factor: 49.962

5.  Aminoacyl-tRNA synthetase families and their significance to the origin of the genetic code.

Authors:  R Wetzel
Journal:  Orig Life       Date:  1978-09

6.  Purification of leucyl transfer ribonucleic acid synthetase from Escherichia coli.

Authors:  H Hayashi; J R Knowles; J R Katze; J Lapointe; D Söll
Journal:  J Biol Chem       Date:  1970-03-25       Impact factor: 5.157

7.  Isolation and partial characterization of temperature-sensitive Escherichia coli mutants with altered leucyl- and seryl-transfer ribonucleic acid synthetases.

Authors:  B Low; F Gates; T Goldstein; D Söll
Journal:  J Bacteriol       Date:  1971-11       Impact factor: 3.490

8.  DNA sequencing with chain-terminating inhibitors.

Authors:  F Sanger; S Nicklen; A R Coulson
Journal:  Proc Natl Acad Sci U S A       Date:  1977-12       Impact factor: 11.205

9.  R factor proteins synthesized in Escherichia coli minicells: incorporation studies with different R factors and detection of deoxyribonucleic acid-binding proteins.

Authors:  S B Levy
Journal:  J Bacteriol       Date:  1974-12       Impact factor: 3.490

10.  Three suppressor mutations which cure a mitochondrial RNA maturase deficiency occur at the same codon in the open reading frame of the nuclear NAM2 gene.

Authors:  M Labouesse; C J Herbert; G Dujardin; P P Slonimski
Journal:  EMBO J       Date:  1987-03       Impact factor: 11.598
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  13 in total

1.  Intron locations and functional deletions in relation to the design and evolution of a subgroup of class I tRNA synthetases.

Authors:  P Schimmel; A Shepard; K Shiba
Journal:  Protein Sci       Date:  1992-10       Impact factor: 6.725

Review 2.  Linkage map of Escherichia coli K-12, edition 10: the traditional map.

Authors:  M K Berlyn
Journal:  Microbiol Mol Biol Rev       Date:  1998-09       Impact factor: 11.056

Review 3.  Linkage map of Escherichia coli K-12, edition 8.

Authors:  B J Bachmann
Journal:  Microbiol Rev       Date:  1990-06

4.  Nuclear gene for mitochondrial leucyl-tRNA synthetase of Neurospora crassa: isolation, sequence, chromosomal mapping, and evidence that the leu-5 locus specifies structural information.

Authors:  C M Chow; R L Metzenberg; U L Rajbhandary
Journal:  Mol Cell Biol       Date:  1989-11       Impact factor: 4.272

5.  Sequence and structural similarities between the leucine-specific binding protein and leucyl-tRNA synthetase of Escherichia coli.

Authors:  R M Williamson; D L Oxender
Journal:  Proc Natl Acad Sci U S A       Date:  1990-06       Impact factor: 11.205

6.  Cysteinyl-tRNA synthetase: determination of the last E. coli aminoacyl-tRNA synthetase primary structure.

Authors:  G Eriani; G Dirheimer; J Gangloff
Journal:  Nucleic Acids Res       Date:  1991-01-25       Impact factor: 16.971

7.  Structural similarities in glutaminyl- and methionyl-tRNA synthetases suggest a common overall orientation of tRNA binding.

Authors:  J J Perona; M A Rould; T A Steitz; J L Risler; C Zelwer; S Brunie
Journal:  Proc Natl Acad Sci U S A       Date:  1991-04-01       Impact factor: 11.205

8.  Isolation and characterization of the gene coding for Escherichia coli arginyl-tRNA synthetase.

Authors:  G Eriani; G Dirheimer; J Gangloff
Journal:  Nucleic Acids Res       Date:  1989-07-25       Impact factor: 16.971

9.  Isolation and characterization of an Escherichia coli seryl-tRNA synthetase mutant with a large increase in Km for serine.

Authors:  J C Willison; M Härtlein; R Leberman
Journal:  J Bacteriol       Date:  1995-06       Impact factor: 3.490

10.  Maize mitochondrial seryl-tRNA synthetase recognizes Escherichia coli tRNA(Ser) in vivo and in vitro.

Authors:  J Rokov; D Söll; I Weygand-Durasević
Journal:  Plant Mol Biol       Date:  1998-10       Impact factor: 4.076
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