Literature DB >> 3307206

New detection method for uropepsinogen (PGA) using isoelectric focusing and immunoblotting techniques.

T Yasuda, Y Ikehara, W Sato, K Kishi.   

Abstract

Uropepsinogen (PGA) was isolated and purified from human urine using a column chromatography series. The purified PGA was injected into a rabbit and a PGA-specific antibody was obtained. PGA isozymogen in human urine could be detected reproducibly by immunoblotting using this antibody after isoelectric focusing electrophoresis (IEF) on polyacrylamide gels. This technique may prove to be useful in the genetic study of PGA polymorphism.

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Year:  1987        PMID： 3307206     DOI： 10.1007/BF00200337

Source DB:  PubMed          Journal:  Z Rechtsmed        ISSN： 0044-3433


  9 in total

1.  Protein measurement with the Folin phenol reagent.

Authors:  O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal:  J Biol Chem       Date:  1951-11       Impact factor: 5.157

2.  Newly characterized genetic polymorphism of uropepsinogen group A (PGA) using both isoelectric focusing and immunoblotting.

Authors:  K Kishi; T Yasuda
Journal:  Hum Genet       Date:  1987-03       Impact factor: 4.132

3.  Variable numbers of pepsinogen genes are located in the centromeric region of human chromosome 11 and determine the high-frequency electrophoretic polymorphism.

Authors:  R T Taggart; T K Mohandas; T B Shows; G I Bell
Journal:  Proc Natl Acad Sci U S A       Date:  1985-09       Impact factor: 11.205

4.  Pepsinogens: genetic polymorphism in man.

Authors:  I M Samloff; P L Townes
Journal:  Science       Date:  1970-04-03       Impact factor: 47.728

5.  Uropepsinogen in human urine: its protein nature, activation and enzymatic properties of activated enzyme.

Authors:  N Minamiura; K Ito; M Kobayashi; O Kobayashi; T Yamamoto
Journal:  J Biochem       Date:  1984-10       Impact factor: 3.387

6.  Primary structure of human pepsinogen gene.

Authors:  K Sogawa; Y Fujii-Kuriyama; Y Mizukami; Y Ichihara; K Takahashi
Journal:  J Biol Chem       Date:  1983-04-25       Impact factor: 5.157

7.  Genetics of urinary pepsinogen: a new hypothesis.

Authors:  R R Frants; J C Pronk; G Pals; J Defize; B D Westerveld; S G Meuwissen; J Kreuning; A W Eriksson
Journal:  Hum Genet       Date:  1984       Impact factor: 4.132

8.  Genetics of pepsinogen I.

Authors:  L Korsnes; T Gedde-Dahl
Journal:  Ann Hum Genet       Date:  1980-01       Impact factor: 1.670

9.  Urinary pepsinogen isozymes: a highly polymorphic locus in man.

Authors:  R T Taggart; R C Karn; A D Merritt; P L Yu; P M Conneally
Journal:  Hum Genet       Date:  1979-11       Impact factor: 4.132
  9 in total
  2 in total

1.  Genetic polymorphism of human serum ribonuclease I (RNase I).

Authors:  T Yasuda; W Sato; K Mizuta; K Kishi
Journal:  Am J Hum Genet       Date:  1988-04       Impact factor: 11.025

2.  Genetic polymorphism of human urine deoxyribonuclease I.

Authors:  K Kishi; T Yasuda; S Awazu; K Mizuta
Journal:  Hum Genet       Date:  1989-02       Impact factor: 4.132
  2 in total

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