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Literature DB >> 2030945

Secondary pulsed field gel electrophoresis: a new method for faster separation of larger DNA molecules.

Abstract

A novel technique, which we call secondary pulsed field gel electrophoresis (SPFG) has been developed. In SPFG, short pulses are applied in the direction of net migration of the DNA in addition to the reorienting pulses used in conventional pulsed field electrophoresis (PFG). Experimental results show that SPFG extends and improves the electrophoretic resolution of DNA for molecules from 0.5 megabase pairs to over 10 megabase pairs in size. This improved resolution is obtained with dramatically shorter run times. Thus SPFG appears to circumvent a number of the key limitations in previous PFG protocols.

Entities: Chemical

Mesh：

Substances：

Year: 1991 PMID： 2030945 PMCID： PMC333856 DOI： 10.1093/nar/19.6.1291

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

11 in total

1. Molecular detrapping and band narrowing with high frequency modulation of pulsed field electrophoresis.

Authors: C Turmel; E Brassard; G W Slater; J Noolandi
Journal: Nucleic Acids Res Date: 1990-02-11 Impact factor: 16.971

2. Theoretical studies of DNA during gel electrophoresis.

Authors: J M Deutsch
Journal: Science Date: 1988-05-13 Impact factor: 47.728

3. High-resolution separation and accurate size determination in pulsed-field gel electrophoresis of DNA. 1. DNA size standards and the effect of agarose and temperature.

Authors: M K Mathew; C L Smith; C R Cantor
Journal: Biochemistry Date: 1988-12-27 Impact factor: 3.162

4. Construction of a Not I restriction map of the fission yeast Schizosaccharomyces pombe genome.

Authors: J B Fan; Y Chikashige; C L Smith; O Niwa; M Yanagida; C R Cantor
Journal: Nucleic Acids Res Date: 1989-04-11 Impact factor: 16.971

5. An electrophoretic karyotype of Neurospora crassa.

Authors: M J Orbach; D Vollrath; R W Davis; C Yanofsky
Journal: Mol Cell Biol Date: 1988-04 Impact factor: 4.272

6. Optimized conditions for pulsed field gel electrophoretic separations of DNA.

Authors: B W Birren; E Lai; S M Clark; L Hood; M I Simon
Journal: Nucleic Acids Res Date: 1988-08-11 Impact factor: 16.971

7. High-resolution separation and accurate size determination in pulsed-field gel electrophoresis of DNA. 2. Effect of pulse time and electric field strength and implications for models of the separation process.

Authors: M K Mathew; C L Smith; C R Cantor
Journal: Biochemistry Date: 1988-12-27 Impact factor: 3.162

8. High-resolution separation and accurate size determination in pulsed-field gel electrophoresis of DNA. 3. Effect of electrical field shape.

Authors: C R Cantor; A Gaal; C L Smith
Journal: Biochemistry Date: 1988-12-27 Impact factor: 3.162

Secondary pulsed field gel electrophoresis: a new method for faster separation of larger DNA molecules.

1. Molecular detrapping and band narrowing with high frequency modulation of pulsed field electrophoresis.

2. Theoretical studies of DNA during gel electrophoresis.

3. High-resolution separation and accurate size determination in pulsed-field gel electrophoresis of DNA. 1. DNA size standards and the effect of agarose and temperature.

4. Construction of a Not I restriction map of the fission yeast Schizosaccharomyces pombe genome.

5. An electrophoretic karyotype of Neurospora crassa.

6. Optimized conditions for pulsed field gel electrophoretic separations of DNA.

7. High-resolution separation and accurate size determination in pulsed-field gel electrophoresis of DNA. 2. Effect of pulse time and electric field strength and implications for models of the separation process.

8. High-resolution separation and accurate size determination in pulsed-field gel electrophoresis of DNA. 3. Effect of electrical field shape.

9. New techniques for purifying large DNAs and studying their properties and packaging.

10. An electrophoretic karyotype for Schizosaccharomyces pombe by pulsed field gel electrophoresis.

1. Methods for pulsed-field gel electrophoresis.

Review 2. The use of karyotyping in the systematics of yeasts.