Literature DB >> 32840778

Branch Migration Activity of Rad54 Protein.

Olga M Mazina1, Alexander V Mazin2.   

Abstract

Rad54 is a eukaryotic protein that plays an important role in homologous recombination. Rad54, a member of the Swi2/Snf2 family, binds to Holliday junctions with high specificity and promotes their branch migration in an ATP hydrolysis-dependent manner. Here we describe the methods our laboratory used to characterize the branch migration activity of Rad54. These assays are applicable for other branch migration proteins regardless of whether they have canonical helicase activity or not.

Entities:  

Keywords:  Branch migration; Holliday junctions; Homologous recombination; Rad54 protein

Mesh：

Substances：

Year:  2021        PMID： 32840778      PMCID： PMC8168686          DOI： 10.1007/978-1-0716-0644-5_11

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  12 in total

1.  Holliday junction branch migration and resolution assays.

Authors:  Angelos Constantinou; Stephen C West
Journal:  Methods Mol Biol       Date:  2004

2.  Polarity and bypass of DNA heterology during branch migration of Holliday junctions by human RAD54, BLM, and RECQ1 proteins.

Authors:  Olga M Mazina; Matthew J Rossi; Julianna S Deakyne; Fei Huang; Alexander V Mazin
Journal:  J Biol Chem       Date:  2012-02-22       Impact factor: 5.157

Review 3.  Repair of strand breaks by homologous recombination.

Authors:  Maria Jasin; Rodney Rothstein
Journal:  Cold Spring Harb Perspect Biol       Date:  2013-11-01       Impact factor: 10.005

4.  Structural biology: snapshots of DNA repair.

Authors:  Stephen C Kowalczykowski
Journal:  Nature       Date:  2008-05-22       Impact factor: 49.962

5.  Visualization of Rad54, a chromatin remodeling protein, translocating on single DNA molecules.

Authors:  Ichiro Amitani; Ronald J Baskin; Stephen C Kowalczykowski
Journal:  Mol Cell       Date:  2006-07-07       Impact factor: 17.970

6.  Catalysis of homologous DNA pairing by yeast Rad51 and Rad54 proteins.

Authors:  G Petukhova; S Stratton; P Sung
Journal:  Nature       Date:  1998-05-07       Impact factor: 49.962

7.  Rad54 protein promotes branch migration of Holliday junctions.

Authors:  Dmitry V Bugreev; Olga M Mazina; Alexander V Mazin
Journal:  Nature       Date:  2006-07-02       Impact factor: 49.962

8.  Reconstituting the 4-Strand DNA Strand Exchange.

Authors:  Olga M Mazina; Alexander V Mazin
Journal:  Methods Enzymol       Date:  2018-02-01       Impact factor: 1.600

9.  Interactions of human rad54 protein with branched DNA molecules.

Authors:  Olga M Mazina; Matthew J Rossi; Nicolas H Thomaä; Alexander V Mazin
Journal:  J Biol Chem       Date:  2007-05-31       Impact factor: 5.157

10.  Analyzing the branch migration activities of eukaryotic proteins.

Authors:  Matthew J Rossi; Olga M Mazina; Dmitry V Bugreev; Alexander V Mazin
Journal:  Methods       Date:  2010-02-16       Impact factor: 3.608
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