| Literature DB >> 32483363 |
Xiaohui Zhang1, Biyun Zhu1, Liang Chen1, Ling Xie1, Weishi Yu1,2, Ying Wang3, Linxi Li1, Shuming Yin1, Lei Yang1, Handan Hu1, Honghui Han4, Yongmei Li1, Liren Wang1, Geng Chen1, Xueyun Ma1, Hongquan Geng5, Wanfeng Huang1, Xiufeng Pang1, Zuozhen Yang2, Yuxuan Wu1, Stefan Siwko6, Ryo Kurita7, Yukio Nakamura8, Li Yang3, Mingyao Liu1, Dali Li9.
Abstract
Although base editors are useful tools for precise genome editing, current base editors can only convert either adenines or cytosines. We developed a dual adenine and cytosine base editor (A&C-BEmax) by fusing both deaminases with a Cas9 nickase to achieve C-to-T and A-to-G conversions at the same target site. Compared to single base editors, A&C-BEmax's activity on adenines is slightly reduced, whereas activity on cytosines is higher and RNA off-target activity is substantially decreased.Entities:
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Year: 2020 PMID： 32483363 DOI： 10.1038/s41587-020-0527-y
Source DB: PubMed Journal: Nat Biotechnol ISSN： 1087-0156 Impact factor: 54.908